LOCUS       sequence-31583-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NKTR
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             637..654
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(707..725)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      721..768
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(892..911)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggttccgc gtggtagtcc acagtgccat ttcgacattg aaatcaaccg
      121 tgagccagtt gggcggataa tgtttcagct gttcagcgac atttgcccga aaacttgcaa
      181 aaacttcctg tgtctgtgta gtggcgaaaa aggtctggga aaaaccaccg gtaaaaaact
      241 gtgctacaaa ggatcaacat tccatcgcgt ggtgaaaaac tttatgatcc agggcggcga
      301 tttttccgaa ggtaacggca agggtgggga atccatttat ggcggctatt ttaaagatga
      361 aaattttatc ctgaaacatg atcgcgcctt cctgctaagc atggcgaatc gtggtaaaca
      421 cacgaatggg agccagttct ttattacgac caaaccggcc ccacacctgg atggagtgca
      481 tgtggtgttt ggtttggtaa tctccggttt tgaagtcatc gaacagattg aaaacctgaa
      541 aacggatgcc gcaagtcgcc cgtatgccga tgtgcgcgtt attgattgtg gcgttttggc
      601 gaccaaatga tgacgaagct tgcggccgca ctcgagcacc accaccacca ccactgagat
      661 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
      721 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
      781 actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
      841 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
      901 ctttcttccc ttcctttctc gccacgttcg
//
