LOCUS       sequence-315830-        1425 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GCaMPer calcium sensor (endoplasmic reticulum).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Werley CA, Boccardo S, Rigamonti A, Hansson EM, Cohen AE
  TITLE     Multiplexed Optical Sensors in Arrayed Islands of Cells for 
            multimodal recordings of cellular physiology.
  JOURNAL   Nat Commun. 2020 Aug 4;11(1):3881. doi: 10.1038/s41467-020-17607-5.
  PUBMED    32753572
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-08-4"; volume: "11(1):3881. doi"; pages: " 
            10.1038/s41467-020-17607-5"
COMMENT     Sequence Label: pMOS003: GCaMPer calcium sensor (endoplasmic
            r...
FEATURES             Location/Qualifiers
     source          1..1425
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(52..60,61..78,79..81,82..114,115..117,118..141,
                     142..168,169..225,226..231,232..501,502..519,520..954,
                     955..957,958..960,961..1401)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNTRDQLTEEQIAEFKEAFSLFDKGGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDAGGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKGGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGGGQVNYEEFVQMMTAK"
     CDS             join(52..60,61..78,79..81,82..114,115..117,118..141,
                     142..168,169..225,226..231,232..501,502..519,520..954,
                     955..957,958..960,961..1401)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNTRDQLTEEQIAEFKEAFSLFDKGGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDAGGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKGGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGGGQVNYEEFVQMMTAK"
     regulatory      148..157
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     438..459
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(565..586)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(826..845)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgctgctat ccgtgccgtt gctgctcggc ctcctcggcc tggccgtcgc catgggttct
       61 catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat gggtcgggat
      121 ctgtacgacg atgacgataa ggatctcgcc accatggtcg actcatcacg tcgtaagtgg
      181 aataagacag gtcacgcagt cagagctata ggtcggctga gctcactcga gaacgtctat
      241 atcaaggccg acaagcagaa gaacggcatc aaggcgaact tcaagatccg ccacaacatc
      301 gaggacggcg gcgtgcagct cgcctaccac taccagcaga acacccccat cggcgacggc
      361 cccgtgctgc tgcccgacaa ccactacctg agcgtgcagt ccaaactttc gaaagacccc
      421 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
      481 ggcatggacg agctgtacaa gggcggtacc ggagggagca tggtgagcaa gggcgaggag
      541 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      601 ttcagcgtgt ccggcgaggg tgagggcgat gccacctacg gcaagctgac cctgaagttc
      661 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
      721 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      781 gccatgcccg aaggctacat ccaggagcgc accatcttct tcaaggacga cggcaactac
      841 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      901 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caacacgcgt
      961 gaccaactga ctgaagagca gatcgcagaa tttaaagagg ctttctccct atttgacaag
     1021 ggcggggatg ggacaataac aaccaaggag ctggggacgg tgatgcggtc tctggggcag
     1081 aaccccacag aagcagagct gcaggacatg atcaatgaag tagatgccgg cggtgacggc
     1141 acaatcgact tccctgagtt cctgacaatg atggcaagaa aaatgaaaga cacagacagt
     1201 gaagaagaaa ttagagaagc gttccgtgtg tttgataagg gtggcaatgg ctacatcagt
     1261 gcagcagagc ttcgccacgt gatgacaaac cttggagaga agttaacaga tgaagaggtt
     1321 gatgaaatga tcagggaagc agacatcgat gggggtggtc aggtaaacta cgaagagttt
     1381 gtacaaatga tgacagcgaa gacagcagaa aaagatgagt tgtag
//
