LOCUS       sequence-31584-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CDC34
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             651..668
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(721..739)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      735..782
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(906..925)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggttccgcg tggtagtccc agctcgcaga aggcgctgct gctggagctc
      121 aaggggctgc aggaagagcc ggtcgaggga ttccgcgtga cactggtgga cgagggcgat
      181 ctatacaact gggaggtggc catcttcggg ccccccaaca cctactacga gggcggctac
      241 ttcaaggcgc gcctcaagtt ccccatcgac tacccatact ctccaccagc ctttcggttc
      301 ctgaccaaga tgtggcaccc taacatctac gagacggggg acgtgtgtat ctccatcctc
      361 cacccgccgg tggacgaccc ccagagtggg gagctgccct cagagaggtg gaaccccacg
      421 cagaacgtca ggaccattct cctgagtgtg atctccctcc tgaacgagcc caacaccttc
      481 tcgcccgcaa acgtggacgc ctccgtgatg tacaggaagt ggaaagagag caaggggaag
      541 gatcgggagt acacagacat catccggaag caggtcctgg ggaccaaggt ggacgcggag
      601 cgtgacggcg tgaaggtgcc ctgatgacga agcttgcggc cgcactcgag caccaccacc
      661 accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
      721 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
      781 tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt
      841 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
      901 gcccgctcct ttcgctttct tcccttcctt tctcgccacg tcgcg
//
