LOCUS       sequence-31585-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UBCi
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..96
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             627..644
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(697..715)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      711..758
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tagaaataat tttgtttaac tttaagaagg agatatacca tgggcagcag ccatcatcat
       61 catcatcaca gcagcggcct ggttccgcgt ggtagtggtg cagtgtctgg gtcagtgcaa
      121 gcttcagata gacttatgaa agagctcagg gacatataca gatcacagag ttataaaaca
      181 gggatttatt cagtggaact cataaatgac agtttatatg actggcatgt taaactgcag
      241 aaggttgacc ctgatagtcc tttgcacagt gatcttcaga tcttaaaaga aaaagaaggc
      301 atagaatata ttttgcttaa cttctctttt aaggataact ttccatttga tcctccattt
      361 gttcgagtgg tgttacctgt tctctcagga gggtatgtat tgggtggagg agcattatgt
      421 atggaacttc tcacaaaaca gggctggagc agtgcctact caatagaatc ggtcatcatg
      481 caaataaatg ccaccttagt caaaggcaaa gccagagtgc agtttggagc aaataagaat
      541 caatataatc tagcaagagc ccaacaatcc tataattcca ttgtacagat acatgagtga
      601 tgacgaagct tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta
      661 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
      721 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
      781 gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
      841 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttc
//
