LOCUS       sequence-315856-        2769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Peredox NADH/NAD+ sensor (cytosolic).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Werley CA, Boccardo S, Rigamonti A, Hansson EM, Cohen AE
  TITLE     Multiplexed Optical Sensors in Arrayed Islands of Cells for 
            multimodal recordings of cellular physiology.
  JOURNAL   Nat Commun. 2020 Aug 4;11(1):3881. doi: 10.1038/s41467-020-17607-5.
  PUBMED    32753572
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-08-4"; volume: "11(1):3881. doi"; pages: " 
            10.1038/s41467-020-17607-5"
COMMENT     Sequence Label: pMOS023: Peredox NADH/NAD+ sensor (cytosolic)
FEATURES             Location/Qualifiers
     source          1..2769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(13..624,625..645,646..1380,1381..1383,1384..2013,
                     2014..2058)
                     /codon_start=1
                     /product="genetically encoded fluorescent biosensor of the 
                     cytosolic NADH-NAD+ redox state (Hung et al., 2011)"
                     /label=Peredox
                     /note="consists of two pH-insensitive mutant copies of the 
                     Thermus aquaticus redox-sensing repressor Rex (T-Rex) 
                     separated by circularly permuted monomeric T-Sapphire 
                     (cpmTS), a pH-resistant GFP variant"
                     /translation="KVPEAAISRLITYLRILEELEAQGVHRTASEQLGELAQVTAFQVD
                     KDLSYFGSYGTDGVGYTVPVLKRELRHILGLNRKWGLCIVGMGRLGSALADWPGFGESF
                     ELRGFFDVDPGMVGRPVRGGVIEHVDLLPQRVPGRIEIALLTVPREAAQKAADLLVAAG
                     IKGILNFAPVVLEVPKEVAVENVDILAGLTRLSFAILNPTWSAAGGHGFTAHNVYIMAD
                     KQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSIQSKLSKDPNEK
                     RDHMVLLEFVTAAGITHGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFS
                     VSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVMVFARYPDHMKQHDFFKSA
                     MPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNTK
                     VPEAAISRLITYLRILEELEAQGVHRTASEQLGELAQVTAFQVDEDLSYFGSYGTDGVG
                     YTVPVLKRELRHILGLNRKWGLCIVGMGRLGSALADWPGFGESFELRGFFDVDPGMVGR
                     PVRGGVIEHVDLLPQRVPGRIEIALLTVPREAAQKAADLLVAAGIKGILNFAPVVLEVP
                     KEVAVENVDFLAGLTRLSFAILNPKWREEMMGSGTGGNASDGGGSGG"
     CDS             2059..2769
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2204..2222)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2472..2491
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggttgcta gcaaagttcc tgaagcagcc atttccagac tgattactta cctgagaatt
       61 ctggaagagc tggaagccca aggtgtacac cgcaccgcct ccgagcaact gggcgagctg
      121 gcccaggtca ccgccttcca ggttgataag gacctgtcct acttcggctc ctacggcact
      181 gacggcgttg gctacactgt accggtcctc aagagagaac tgcgccatat cctcggtctg
      241 aaccgcaaat ggggcctgtg catcgtgggg atgggccgcc tgggatccgc tcttgctgat
      301 tggcctggtt ttggcgagag cttcgagctg cgcggtttct tcgatgttga cccaggtatg
      361 gttggtcggc cggttcgcgg tggtgttatc gaacacgttg atctgctgcc ccagcgcgta
      421 cctggtcgca tcgaaatcgc tctgctgacg gttccacgcg aggcagcaca gaaggctgcc
      481 gacctgctgg ttgccgctgg catcaagggc atcctgaact tcgctccagt tgtactggag
      541 gttcccaagg aagtggctgt tgagaacgtt gacatcctgg ccggtctgac gcgtctgagc
      601 tttgccattc tgaaccccac gtggagcgca gcaggtgggc atggtttcac tgctcacaac
      661 gtgtatatca tggctgacaa acagaagaac ggtatcaagg ccaacttcaa gatccgccac
      721 aacatcgaag acggcggcgt tcagctggct gatcactacc agcagaacac cccaatcggc
      781 gacggccctg ttctgctgcc tgacaaccac tacttaagca tccagtctaa actgtccaaa
      841 gacccaaacg agaagcgcga ccacatggtt ctgctggaat ttgttaccgc cgctggtatc
      901 acccacggca tggacgaact gtacaagggc ggcaccgggg gatctatggt ttccaaaggc
      961 gaggaactgt tcactggtgt tgttcctatc ctggttgaac tggacggcga cgttaacggc
     1021 cacaaattca gcgttagcgg ggaaggcgaa ggagacgcta cctacggcaa gctgactctg
     1081 aagttcatct gcaccactgg caagctgcct gttccttggc ctaccctggt tactaccttc
     1141 tcctatggtg ttatggtttt cgccagatat cctgaccaca tgaagcagca cgacttcttc
     1201 aagagcgcca tgcctgaggg ctacgttcag gaacgcacca tctttttcaa ggacgacggc
     1261 aactataaga cacgcgctga ggttaagttc gagggcgaca ctctggttaa ccgcatcgag
     1321 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg gccataagct tgaatataac
     1381 accaaggtac ctgaagccgc catcagccgc ctcatcactt acctccgcat cctggaggag
     1441 ctcgaagccc aaggcgttca ccgcactgca agcgagcagc tgggcgaact ggcacaggtt
     1501 acagcattcc aggttgacga agatctgtcc tatttcggtt cctacggcac cgacggtgtt
     1561 ggctacacag tccctgtcct gaagcgcgag ctgcgccaca tcctgggcct gaaccgcaag
     1621 tggggcttat gcatcgttgg catgggccgc ctcggtagtg cactggcaga ctggccgggc
     1681 tttggtgaat ctttcgaact gcgcggcttc ttcgacgttg atccaggcat ggttgggcgc
     1741 cctgttcgcg gtggcgttat cgagcatgtt gacctcctgc cacagcgcgt cccgggtcgc
     1801 atcgagatcg ccctgctgac cgttcctcgc gaagctgcac agaaggcagc cgacttacta
     1861 gtcgccgctg gtatcaaggg aattctgaac ttcgcccctg ttgttctgga ggttcctaag
     1921 gaagttgctg ttgaaaacgt tgatttcctg gccggcctga cccgcctgag cttcgcaatc
     1981 ctgaatccta agtggcgcga agaaatgatg ggttccggaa ctgggggcaa cgcctctgac
     2041 ggtggtgggt ctggtggtat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
     2101 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     2161 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     2221 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     2281 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     2341 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     2401 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     2461 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     2521 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     2581 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     2641 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     2701 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgaactg
     2761 tacaagtga
//
