LOCUS       sequence-315858-        1740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PercevalHR ATP/ADP sensor (cytosolic).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Werley CA, Boccardo S, Rigamonti A, Hansson EM, Cohen AE
  TITLE     Multiplexed Optical Sensors in Arrayed Islands of Cells for 
            multimodal recordings of cellular physiology.
  JOURNAL   Nat Commun. 2020 Aug 4;11(1):3881. doi: 10.1038/s41467-020-17607-5.
  PUBMED    32753572
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-08-4"; volume: "11(1):3881. doi"; pages: " 
            10.1038/s41467-020-17607-5"
COMMENT     Sequence Label: pMOS007: PercevalHR ATP/ADP sensor (cytosolic)
FEATURES             Location/Qualifiers
     source          1..1740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             193..444
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSFQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     381..402
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(505..526)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(766..785)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgaaaaagg ttgaatccat catcaggccc gaaaagctgg agatcgttaa gaaggctctc
       61 tcggacgctg gatatgtggg tatgaccgtc tctgaagtca aaggctctgg cgtccagggc
      121 ggcatcttcg agaggtaccg aggaagggtg tactctgcag gctacaacag cgacaacgtc
      181 tacatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
      241 atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      301 ggccccgtgc tgctgcccga caaccactac ctgagcttcc agtccaagct gagcaaagac
      361 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      421 ctcggcatgg acgagctgta caagggcggt tccggaggca tggtgagcaa gggcgaggag
      481 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      541 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg
      601 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac
      661 ggtctccaat gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      721 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      781 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      841 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agcttgagta caacggcacc
      901 atagtagatc tgatccctaa ggtaaaaatt gaactagtgg tgaaggagga ggacgtcgat
      961 aacgtgatag acattatttg cgaaaatgcc cgcacaggta accccggcga cggtaagata
     1021 tttgtgatcc cagtggagcg agtggtcagg gtgcgaacca aagaggaggg agcatctggt
     1081 ggtggatccg gtggtggcgg tgcatctggt atgaaaaagg ttgaagctat tattcgccca
     1141 gagaaactgg agattgttaa gaaggccctg tccgacgccg gctatgttgg catgaccgtt
     1201 tccgaggtta aaggccgcgg tgccggcggt ggcgacctga ttcctaaggt gaagatcgag
     1261 ctggttgtta aggaggaaga cgttgataat gttatcgaca ttatctgcga gaacgcccgc
     1321 actggcaatc ctggtgacgg caaaatcttc gttataccgg tcgagcgaat tgtgcgagta
     1381 cgaaccaaag aagagggtgc ctccggcggt ggcggtggct ccggtggcgc cagcggcatg
     1441 aagaaggttg aggccatcat ccgcccagag aagctggaaa tcgttaagaa ggccttaaac
     1501 gacgacggct acgtcggcat gacagttagc gaggttaaag gtcgcggtgc cggtgggggt
     1561 gatctgattc ctaaggttaa aatcgagctc gttgttaagg aggaggacgt tgacaacatt
     1621 atcgatatca tctgcgaaaa cgctcgcacc ggcaacccag gcgacggcaa gattttcgtc
     1681 attcctgttg agagagtggt gcgtccccgt acgaaggagg agggcaagga agcactgtga
//
