LOCUS       sequence-315860-        1557 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Werley CA, Boccardo S, Rigamonti A, Hansson EM, Cohen AE
  TITLE     Multiplexed Optical Sensors in Arrayed Islands of Cells for 
            multimodal recordings of cellular physiology.
  JOURNAL   Nat Commun. 2020 Aug 4;11(1):3881. doi: 10.1038/s41467-020-17607-5.
  PUBMED    32753572
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-08-4"; volume: "11(1):3881. doi"; pages: " 
            10.1038/s41467-020-17607-5"
COMMENT     Sequence Label: pMOS009E: Entry vector for: QuasAr2-mOrange2 v...
FEATURES             Location/Qualifiers
     source          1..1557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..759
                     /codon_start=1
                     /product="achaerhodopsin-3 (Arch) derivative with improved 
                     voltage sensitivity (Hochbaum et al., 2014)"
                     /label=QuasAr2
                     /translation="MVSIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVT
                     DKDAREYYAVTILVSGIASAAYLSMFFGIGLTEVSVGGEMLDIYYARYAQWLFTTPLLL
                     LHLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYVLA
                     TSLRSAAKERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLD
                     VTAKVGFGFILLRSRAILGDTEAPEPSAGAD"
     CDS             766..1473
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEYNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 atggtaagta tcgctctgca ggctggttac gacctactgg gtgacggcag acctgaaacc
       61 ctgtggctgg gcatcggcac tctgctgatg ctgattggaa ccttctactt tctggtccgc
      121 ggatggggag tcaccgataa ggatgcccgg gaatattacg ctgtgactat cctggtgtcc
      181 ggaatcgcat ccgccgcata tctgtctatg ttctttggta tcgggcttac tgaggtgtcc
      241 gtcgggggcg aaatgttgga tatctattat gccaggtacg cccagtggct gtttaccacc
      301 ccacttctgc tgctgcacct ggcccttctc gctaaggtgg atcgggtgac catcggcacc
      361 ctggtgggtg tggacgccct gatgatcgtc actggcctca tcggagcctt gagccacacg
      421 gccatagcca gatacagttg gtggttgttc tctacaattt gcatgatagt ggtgctctat
      481 gttctggcta catccctgcg atctgctgca aaggagcggg gccccgaggt ggcatctacc
      541 tttaacaccc tgacagctct ggtcttggtg ctgtggaccg cttaccctat cctgtggatc
      601 ataggcactg agggcgctgg cgtggtgggc ctgggcatcg aaactctgct gtttatggtg
      661 ttggacgtga ctgccaaggt cggctttggc tttatcctgt tgagatcccg ggctattctg
      721 ggcgacaccg aggcaccaga acccagtgcc ggtgccgata cgttgatggt gagcaagggc
      781 gaggagtata acatggccat catcaaggag ttcatgcgct tcaaggtgcg catggagggc
      841 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      901 tttcagaccg ctaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      961 ctgtcccctc atttcaccta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     1021 gactacttca agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaactacgag
     1081 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     1141 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtgatgca gaagaagacc
     1201 atgggctggg aggcctcctc cgagcggatg taccccgagg acggtgccct gaagggcaag
     1261 atcaagatga ggctgaagct gaaggacggc ggccactaca cctccgaggt caagaccacc
     1321 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca tcgtcgacat caagttggac
     1381 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     1441 cactccaccg gcggcatgga cgagctgtac aagagtagaa tcacaagcga aggcgagtac
     1501 atccccctgg atcaaataga cataaatgta ggtggatttt gttatgagaa tgaataa
//
