LOCUS       sequence-315866-        1392 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry vector for: Mitycam calcium sensor (mitochondrial).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Werley CA, Boccardo S, Rigamonti A, Hansson EM, Cohen AE
  TITLE     Multiplexed Optical Sensors in Arrayed Islands of Cells for 
            multimodal recordings of cellular physiology.
  JOURNAL   Nat Commun. 2020 Aug 4;11(1):3881. doi: 10.1038/s41467-020-17607-5.
  PUBMED    32753572
REFERENCE   2  (bases 1 to 1392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-08-4"; volume: "11(1):3881. doi"; pages: " 
            10.1038/s41467-020-17607-5"
COMMENT     Sequence Label: pMOS028E: Entry vector for: Mitycam calcium se...
FEATURES             Location/Qualifiers
     source          1..1392
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..75
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             112..189
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             232..483
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSFQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     420..441
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(553..574)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(814..833)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgtccgtcc tgacgccgct gctgctgcgg ggcttgacag gctcggcccg gcggctccca
       61 gtgccgcgcg ccaagatcca ttcgttgccg ccggagggga agcttggcat gaagaggcgc
      121 tggaagaaaa acttcattgc cgtcagcgct gccaaccggt tcaagaagat ctccagctcc
      181 ggggcactgg ggtctgcagg ctacaacagc accaacgtct atatcatggc cgacaagcag
      241 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag
      301 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
      361 aaccactacc tgagcttcca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
      421 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
      481 aaggtcgacg gtggcagcgg tggcaccggt gtgagcaagg gcgaggagct gttcaccggg
      541 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      601 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctcat ctgcaccacc
      661 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgaagtgc
      721 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      781 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      841 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      901 aaggaggacg gcaacatcct ggggcacaag ctggagtaca acggtaccga ccaactgaca
      961 gaagagcaga ttgcagagtt caaagaagcc ttctcattat tcgacaagga tggggacggc
     1021 accatcacca caaaggaact tggcaccgtt atgaggtcgc ttggacaaaa cccaacggaa
     1081 gcagaattgc aggatatgat caatgaagtc gatgctgatg gcaatggaac gatttacttt
     1141 cctcaatttc ttactatgat ggctagaaaa atgaaggaca cagacagcga agaggaaatc
     1201 cgagaagcat tccgtgtttt tgacaaggat gggaacggct acatcagcgc tgctgaatta
     1261 cgtcacgtca tgacaaacct cggggagaag ttaacagatg aagaagttga tgaaatgata
     1321 agggaagcag atatcgatgg tgatggccaa gtaaactatg aagagtttgt acaaatgatg
     1381 acagcaaagt aa
//
