LOCUS       sequence-31587-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PPIL2
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             652..669
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(722..740)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      736..783
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggttccgc gtggtagtgg ctacgtgcgg ctgcacacca acaagggcga
      121 cctcaacctg gagctgcact gcgacctgac accaaaaacc tgcgaaaact tcatcaggct
      181 ttgcaagaag cattattacg atggcaccat cttccacaga tccatccgga actttgtgat
      241 ccaagggggc gaccccacag gcacaggcac gggtggggag tcatactggg ggaagccctt
      301 caaagacgag ttccggccca acctctcgca cacgggccgc ggcatcctca gcatggccaa
      361 ctccgggccc aacagcaaca ggtctcaatt cttcatcacg tttcgctcct gtgcctacct
      421 ggacaagaag cataccatct ttggacgggt tgttgggggc tttgacgtac tgacagccat
      481 ggagaatgtg gagagtgacc ccaaaactga ccgccctaag gaggagatcc gcattgatgc
      541 cactacagtg ttcgtggacc cctatgagga ggccgatgcc cagattgcgc aggagcggaa
      601 gacacagctc aaggtagccc cgtgatgacg aagcttgcgg ccgcactcga gcaccaccac
      661 caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
      721 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
      781 ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat
      841 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      901 cgcccgctcc tttcgcttct
//
