LOCUS       sequence-315872-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry vector for: superecliptic pHluorin (endoplasmic reticulum).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Werley CA, Boccardo S, Rigamonti A, Hansson EM, Cohen AE
  TITLE     Multiplexed Optical Sensors in Arrayed Islands of Cells for 
            multimodal recordings of cellular physiology.
  JOURNAL   Nat Commun. 2020 Aug 4;11(1):3881. doi: 10.1038/s41467-020-17607-5.
  PUBMED    32753572
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-08-4"; volume: "11(1):3881. doi"; pages: " 
            10.1038/s41467-020-17607-5"
COMMENT     Sequence Label: pMOS031E: Entry vector for: superecliptic pHlu...
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..765
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(82..110)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     705..725
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 atgctgctat ccgtgccgtt gctgctcggc ctcctcggcc tggccgctgc aatgagtaaa
       61 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
      121 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
      181 cttaaattta tttgcactac tggaaaacta cctgttcctt ggccaacact tgtcactact
      241 ttaacttatg gtgttcaatg cttttcaaga tacccagatc atatgaaacg gcatgacttt
      301 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
      361 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
      421 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
      481 aactataacg atcaccaggt gtacatcatg gcagacaaac aaaagaatgg aatcaaagct
      541 aacttcaaaa ttagacacaa cattgaagat ggaggcgttc aactagcaga ccattatcaa
      601 caaaatactc caattggcga tgggcccgtc cttttaccag acaaccatta cctgtttaca
      661 acttctactc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
      721 gtaacagctg ctgggattac acatggcatg gatgaactat acaaaaagga cgagctgtaa
//
