LOCUS       sequence-315878-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry vector for: ratiometric pHluorin (endoplasmic reticulum).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Werley CA, Boccardo S, Rigamonti A, Hansson EM, Cohen AE
  TITLE     Multiplexed Optical Sensors in Arrayed Islands of Cells for 
            multimodal recordings of cellular physiology.
  JOURNAL   Nat Commun. 2020 Aug 4;11(1):3881. doi: 10.1038/s41467-020-17607-5.
  PUBMED    32753572
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-08-4"; volume: "11(1):3881. doi"; pages: " 
            10.1038/s41467-020-17607-5"
COMMENT     Sequence Label: pMOS030E: Entry vector for: ratiometric pHluor...
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..765
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Miesenbock et al., 1998)"
                     /label=ratiometric pHluorin
                     /note="responds to a drop in pH with a reversible decrease 
                     in excitation at 395 nm and a corresponding increase in 
                     excitation at 475 nm"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKDDGNILGHKLEYNYNEHLVYIMADKQKNGTKA
                     IFQVHHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLHTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(82..110)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     705..725
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 atgctgctat ccgtgccgtt gctgctcggc ctcctcggcc tggccgctgc aatgagtaaa
       61 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
      121 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
      181 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact
      241 ttctcttatg gtgttcaatg cttttcaaga tacccagatc atatgaaacg gcatgacttt
      301 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
      361 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
      421 gagttaaaag gtattgattt taaagatgat ggaaacattc ttggacacaa attggaatac
      481 aactataacg agcacttggt gtacatcatg gcagacaaac aaaagaatgg taccaaagct
      541 atctttcaag ttcaccacaa cattgaagat ggaggcgttc aactagcaga ccattatcaa
      601 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgcacaca
      661 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
      721 gtaacagctg ctgggattac acatggcatg gatgaactat acaaaaagga cgagctgtaa
//
