LOCUS       sequence-315884-        1275 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor 
            (endoplasmic reticulum).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Werley CA, Boccardo S, Rigamonti A, Hansson EM, Cohen AE
  TITLE     Multiplexed Optical Sensors in Arrayed Islands of Cells for 
            multimodal recordings of cellular physiology.
  JOURNAL   Nat Commun. 2020 Aug 4;11(1):3881. doi: 10.1038/s41467-020-17607-5.
  PUBMED    32753572
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-08-4"; volume: "11(1):3881. doi"; pages: " 
            10.1038/s41467-020-17607-5"
COMMENT     Sequence Label: pMOS012E: Entry vector for: Grx1-roGFP1-iE_ER
            ...
FEATURES             Location/Qualifiers
     source          1..1275
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             91..117
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(547..549,550..1263)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCESNVYITADKQKNGIK
                     ANFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(589..610)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(850..869)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1200..1221
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgcatcctg ccgtcttcct atccttaccc gacctcagat gctcccttct gctcctggta
       61 acttgggttt ttactcctgt aacaactgaa tatccgtatg atgtgcctga ctacgcagaa
      121 ggtaccatgg ctcaagagtt tgtgaactgc aaaatccagc ctgggaaggt ggttgtgttc
      181 atcaagccca cctgcccgta ctgcaggagg gcccaagaga tcctcagtca attgcccatc
      241 aaacaagggc ttctggaatt tgtcgatatc acagccacca accacactaa cgagattcaa
      301 gattatttgc aacagctcac gggagcaaga acggtgcctc gagtctttat tggtaaagat
      361 tgtataggcg gatgcagtga tctagtctct ttgcaacaga gtggggaact gctgacgcgg
      421 ctaaagcaga ttggagctct gcagactagt ggtggttcag gtggtggtgg ttcaggtggt
      481 ggtggttcag gtggaggagg atcaggagga ggaggatcag gaggaggagg atcaggagga
      541 gaattcgtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
      601 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
      661 tacggcaagc tgaccctgaa gttcatctcc accaccggca agctgcccgt gccctggccc
      721 accctcgtga ccaccctgag ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
      781 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
      841 agcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
      901 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
      961 cacaagctgg agtacaacta caactgcgag agcaacgtct atatcaccgc cgacaagcag
     1021 aagaacggca tcaaggctaa cttcaagacc cgccacaaca tcgaggacgg cagcgtgcag
     1081 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     1141 aaccactacc tgagcacctg ctccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     1201 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     1261 aaggacgaac tgtaa
//
