LOCUS       sequence-315888-        1410 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor 
            (mitochondrial).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Werley CA, Boccardo S, Rigamonti A, Hansson EM, Cohen AE
  TITLE     Multiplexed Optical Sensors in Arrayed Islands of Cells for 
            multimodal recordings of cellular physiology.
  JOURNAL   Nat Commun. 2020 Aug 4;11(1):3881. doi: 10.1038/s41467-020-17607-5.
  PUBMED    32753572
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-08-4"; volume: "11(1):3881. doi"; pages: " 
            10.1038/s41467-020-17607-5"
COMMENT     Sequence Label: pMOS015E: Entry vector for: mito-roGFP2-Orp1 H...
FEATURES             Location/Qualifiers
     source          1..1410
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..75
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             join(100..102,103..813)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(142..163)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(403..422)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     750..771
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgtccgtcc tgacgccgct gctgctgcgg ggcttgacag gctcggcccg gcggctccca
       61 gtgccgcgcg ccaagatcca ttcgttgccg ccgggatccg tgagcaaggg cgaggagctg
      121 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      181 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      241 tccaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
      301 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      361 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
      421 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
      481 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaactgc
      541 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
      601 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
      661 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcacctg ctccgccctg
      721 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
      781 gggatcactc tcggcatgga cgagctgtac aagactagtg gtggttcagg tggtggtggt
      841 tcaggtggtg gtggttcagg tggaggagga tcaggaggag gaggatcagg aggaggagga
      901 tcaggaggag aattcgatat ctcagaattc tataagctag cacctgttga caagaaaggc
      961 caaccattcc ccttcgacca attaaaggga aaagtggtgc ttatcgttaa tgttgcctcc
     1021 aaatgtggat tcactcctca atacaaagaa ctagaggcct tgtacaaacg ttataaggac
     1081 gaaggattta ccatcatcgg gttcccatgc aaccagtttg gccaccaaga acctggctct
     1141 gatgaagaaa ttgcccagtt ctgccaactg aactatggcg tgactttccc cattatgaaa
     1201 aaaattgacg ttaatggtgg caatgaggac cctgtttaca agtttttgaa gagccaaaaa
     1261 tccggtatgt tgggcttgag aggtatcaaa tggaattttg aaaaattctt agtcgataaa
     1321 aagggtaaag tgtacgaaag atactcttca ctaaccaaac cttcttcgtt gtccgaaacc
     1381 atcgaagaac ttttgaaaga ggtggaatag
//
