LOCUS       sequence-31590-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AMPKB2
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..97
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             409..426
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(479..497)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      493..540
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(664..683)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca
       61 tcatcatcac agcagcggcc tggttccgcg tggtagtgta aagcccacac agcaggcccg
      121 gcccactgtt atccgctggt ctgaaggagg caaggaggtc ttcatctctg ggtccttcaa
      181 caattggagc accaagattc cactgattaa gagccataat gactttgttg ccatcctgga
      241 cctccctgag ggagagcacc aatacaagtt ctttgtggat ggacagtggg ttcatgatcc
      301 atcagagcct gtggttacca gtcagcttgg cacaattaac aatttgatcc atgtcaagaa
      361 atctgatttt gaggtgttct gatgacgaag cttgcggccg cactcgagca ccaccaccac
      421 caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
      481 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
      541 ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa
      601 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      661 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      721 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
      781 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
      841 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa ctggaacaca
      901 ctca
//
