LOCUS       sequence-31591-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PPIE
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..89
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             632..649
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(702..720)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      716..763
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(887..906)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aattttgttt aactttaaga aggagatata ccatgggcag cagccatcat catcatcatc
       61 acagcagcgg cctggttccg cgtggtagtc ccattgctaa aaaggcccgc tcaaatcctc
      121 aggtgtacat ggacatcaag attgggaaca agccggctgg ccgcatccag atgctcctgc
      181 gttctgatgt cgtgcccatg acagcagaga atttccgctg cctgtgcact catgaaaagg
      241 gctttggctt taagggaagc agcttccacc gcatcatccc ccagttcatg tgccagggcg
      301 gtgatttcac aaaccacaat ggcactgggg gcaagtccat ctatgggaag aagttcgatg
      361 atgaaaactt tatcctcaag catacgggac caggtctact atccatggcc aactctggcc
      421 caaacaccaa tggctctcag ttcttcctga catgtgacaa gacagactgg ctggatggca
      481 agcatgtggt gtttggagag gtcaccgaag gcctagatgt cttgcggcaa attgaggccc
      541 agggcagcaa ggacgggaag ccaaagcaga aggtgatcat cgccgactgt ggggagtacg
      601 tgtgatgacg aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc
      661 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
      721 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
      781 atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
      841 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
      901 ttcccttcct ttctcgccac gttcgccggc
//
