LOCUS       sequence-31592-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PPIC
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             10..32
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..95
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             689..706
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(759..777)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      773..820
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tagaataatt ttgtttaact ttaagaagga gatataccat gggcagcagc catcatcatc
       61 atcatcacag cagcggcctg gttccgcgtg gtagtggggc cgagggcttc cgcaagcgag
      121 gcccctcggt gacggccaag gtcttctttg atgtgaggat tggagacaaa gatgttggca
      181 gaattgtgat tggcctcttt ggaaaagttg tgcccaagac agtggaaaat tttgttgctc
      241 tagcaacagg agagaaagga tatggatata aaggaagcaa gtttcatcgt gtcatcaagg
      301 atttcatgat tcaaggaggt gacatcacca ctggagatgg cactgggggt gtgagcatct
      361 atggtgagac atttccagat gagaacttca agctgaagca ctatggcatt gggtgggtca
      421 gcatggccaa cgctgggcct gacaccaatg gctctcagtt ctttatcacc ttgaccaagc
      481 ccacctggtt ggacggcaaa catgtggtgt ttggaaaagt cattgatggg atgacagtgg
      541 tgcactccat agagctccaa gcaactgatg ggcatgaccg tccactcacc aactgctcga
      601 tcatcaacag tggcaagata gacgtgaaaa cgccttttgt ggttgagatc gctgattggt
      661 gatgacgaag cttgcggccg cactcgagca ccaccaccac caccactgag atccggctgc
      721 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
      781 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
      841 cggatggcga atgggacgcg ccctgta
//
