LOCUS       sequence-31594-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CAPN1
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 taattttgtt taactttaag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggttcc gcgtggtagt ggccgccatg agaatgccat caaatatctg
      121 ggccaggatt atgagcagct gcgtgtgcgt tgcctgcaga gcggcaccct gtttcgtgat
      181 gaggcctttc cgccggtgcc gcagagcctg ggttataaag acctgggtcc gaatagcagc
      241 aaaacctatg gcatcaaatg gaaacgtccg accgaactgc tgagcaaccc gcagtttatt
      301 gtggatggcg cgacccgcac cgacatctgc cagggcgcac tgggcgactg ctggctgctg
      361 gcggccattg ccagcctgac cctgaacgac accctgctgc atcgtgtggt tccgcatggc
      421 cagagctttc agaatggcta tgccggcatc tttcattttc agctgtggca gtttggcgag
      481 tgggtggacg tggttgtgga tgacctgctg ccgatcaaag acggcaaact ggtgtttgtg
      541 catagcgccg aaggcaacga gttttggagc gccctgctgg agaaagccta tgccaaagtg
      601 aatggcagct atgaggccct gagcggcggt agcaccagcg agggctttga ggactttacc
      661 ggcggtgtta ccgagtggta tgagctgcgc aaagcgccga gcgacctgta tcagatcatc
      721 ctgaaagcgc tggagcgtgg cagcctgctg ggctgcagca ttgacatcag cagcgttctg
      781 gacatggagg ccatcacctt taaaaaactg gtgaaaggcc atgcctatag cgtgaccggc
      841 gccaaacagg tgaactatcg tggccaggtg gttagcctga tccgtatgcg taacccgtgg
      901 ggcgaggtgg agtgac
//
