LOCUS       sequence-31595-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SF3A1
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..21
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..85
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             373..390
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(443..461)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      457..504
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(628..647)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     838..859
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttgtttaact ttaagaagga gatataccat gggcagcagc catcatcatc atcatcacag
       61 cagcggcctg gttccgcgtg gtagtccagt gtccatcaaa gtccaggtgc ccaacatgca
      121 ggataagacg gaatggaaac tgaatgggca ggtgctggtc ttcaccctcc cactcacgga
      181 ccaggtctct gtcattaagg tgaagattca tgaagccaca ggcatgcctg cagggaaaca
      241 gaagctacag tatgagggta tcttcatcaa agattccaac tcactggctt actacaacat
      301 ggccaatggc gcagtcatcc acctggccct caaggagaga ggctgatgac gaagcttgcg
      361 gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag
      421 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
      481 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg
      541 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      601 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      661 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      721 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      781 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      841 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattc
//
