LOCUS       sequence-31597-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SDCCAG10
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             10..32
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..95
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             623..640
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(693..711)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      707..754
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(878..897)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tagaataatt ttgtttaact ttaagaagga gatataccat gggcagcagc catcatcatc
       61 atcatcacag cagcggcctg gttccgcgtg gtagtgagcc tcccacgaat gggaaggttt
      121 tattgaaaac tacagctgga gatattgaca tagagttgtg gtccaaagaa gctcctaaag
      181 cttgcagaaa ttttatccaa ctttgtttgg aagcttatta tgacaatacc atttttcata
      241 gagttgtgcc tggtttcata gtccaaggcg gagatcctac tggcacaggg agtggtggag
      301 agtctatcta tggagcgcca ttcaaagatg aatttcattc acggttgcgt tttaatcgga
      361 gaggactggt tgccatggca aatgctggtt ctcatgataa tggcagccag tttttcttca
      421 cactgggtcg agcagatgaa cttaacaata agcataccat ctttggaaag gttacagggg
      481 atacagtata taacatgttg cgactgtcag aagtagacat tgatgatgac gaaagaccac
      541 ataatccaca caaaataaaa agctgtgagg ttttgtttaa tccttttgat gactgatgac
      601 gaagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa
      661 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
      721 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt
      781 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
      841 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
      901 tttctcgcca cgttcgccgg ct
//
