LOCUS       sequence-31598-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: E2-EPF5
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             585..602
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(655..673)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      669..716
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(840..859)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggttccgcg tggtagtatg aactccaacg tggagaacct acccccgcac
      121 atcatccgcc tggtgtacaa ggaggtgacg acactgaccg cagacccacc cgatggcatc
      181 aaggtctttc ccaacgagga ggacctcacc gacctccagg tcaccatcga gggccctgag
      241 gggaccccat atgctggagg tctgttccgc atgaaactcc tgctggggaa ggacttccct
      301 gcctcccccc ccaagggcta cttcctgacc aagatcttcc acccgaacgt gggcgccaat
      361 ggcgagatct gcgtcaacgt gctcaagagg gactggacgg ctgagctggg catccgacac
      421 gtactgctga ccatcaagtg cctgctgatc caccctaacc ccgagtctgc actcaacgag
      481 gaggcgggcc gcctgctctt ggagaactac gaggagtatg cagctcgggc ccgtctgctc
      541 acagagatcc acgggtgatg acgaagcttg cggccgcact cgagcaccac caccaccacc
      601 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      661 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      721 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
      781 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
      841 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
      901 aaatcggggg ctccctttag ggt
//
