LOCUS       sequence-31599-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FLJ11011
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             468..485
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(538..556)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      552..599
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(723..742)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggttccgcg tggtagtatg gcgtcaatgc agaaacgact acagaaagaa
      121 ctgttggctt tgcaaaatga cccacctcct ggaatgacct taaatgagaa gagtgttcaa
      181 aattcaatta cacagtggat tgtagacatg gaaggtgcac caggtacctt atatgaaggg
      241 gaaaaatttc aacttctatt taaatttagt agtcgatatc cttttgactc tcctcaggtc
      301 atgtttactg gtgaaaatat tcctgttcat cctcatgttt atagcaatgg tcatatctgt
      361 ttatccattc taacagaaga ctggtcccca gcgctctcag tccaatcagt ttgtcttagc
      421 attattagca tgctttcctg atgacgaagc ttgcggccgc actcgagcac caccaccacc
      481 accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
      541 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
      601 tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag
      661 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
      721 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
      781 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
      841 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggttttcgcc
      901 ctttgacgtt ggagtccacg tc
//
