LOCUS       sequence-316005-       14060 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TtgO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3'UTR-Ubiquitin-Renil
            la-attB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14060)
  AUTHORS   Gamez S, Vesga LC, Mendez-Sanchez SC, Akbari OS
  TITLE     Spatial control of gene expression in flies using bacterially 
            derived binary transactivation systems.
  JOURNAL   Insect Mol Biol. 2021 Oct;30(5):461-471. doi: 10.1111/imb.12717. 
            Epub 2021 May 24.
  PUBMED    33963794
REFERENCE   2  (bases 1 to 14060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/imb.12717";
            journalName: "Insect Mol Biol"; date: "2021-10"; volume: "30"; 
            issue: "5"; pages: "461-471"
COMMENT     Sequence Label: 908E
FEATURES             Location/Qualifiers
     source          1..14060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     123..142
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     376..393
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    510..531
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(546..551,552..569,570..576)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    584..600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     589..611
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     608..624
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     608..624
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        645..663
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    882..951
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             2864..3799
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     primer_bind     complement(3813..3832)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    3842..3923
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     3867..3886
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(4727..5440,5441..5443,5444..5446))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTSKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4772..4793)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5121..5140
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5380..5401
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(5447..5500)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(5501..7150)
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     7066..7084
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     protein_bind    complement(7710..7743)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(join(8518..9132,9203..9334,9538..9853,
                     9915..10569,10644..10917,11319..11390))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     11842..11860
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     primer_bind     complement(11893..11912)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11894..11912)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11919..11936)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11919..11935)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11928..11950)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12077..12532
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12164..12183)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12374..12395
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12558..12662
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12663..12731,12732..13523)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12881..12900)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(13694..14060,1..222)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
       61 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      121 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      181 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      241 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      301 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
      361 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
      421 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
      481 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
      541 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
      601 tttcacacag gaaacagcta tgaccatgat tacgccaagc tcgaaattaa ccctcactaa
      661 agggaacaaa agctggctag aactagtgtc gacatgcccg ccgtgaccgt cgagaacccg
      721 ctgacgctgc cccgcgtatc cgcacccgcc gacgccgtcg cacgtcccgt gctcaccgtg
      781 accaccgcgc ccagcggttt cgagggcgag ggcttcccgg tgcgccgcgc gttcgccggg
      841 atcaactacc gccacctcga cccgttcatc atgatggacc agatgggtga ggtggagtac
      901 gcgcccgggg agcccaaggg cacgccctgg cacccgcacc gcggcttcga gaccgtgacc
      961 tacatcgtcg acactagtgg atctctagac gcgcagatcg ccgatgggcg tggcgccgga
     1021 gggcaccttc accttgccgt acggcttgct gttcttcgcg ttcaaaatct ccagctccat
     1081 tttgctttcg gtgcgcttgc aatcagtact gtccaaaatc gaaaatcgcc gaaccgtagt
     1141 gtgaccgtgc ggggctctgc gaaaataaac ttttttaggt atatggccac acacggggaa
     1201 agcacagtgg attatatgtt ttaatattat aatatgcagg ttttcattac ttatccagat
     1261 gtaagcccac ttaaagcgat ttaacaatta tttgccgaaa gagtataaac aaatttcact
     1321 taaaaatgga ttaagaaaag cttgtgtaag attatgcgca gcgttgccag atagctccat
     1381 ttaaaacact tcaaaaacaa taagttttga aaatatatac ataaatagca gtcgttgccg
     1441 caacgctcaa cacatcacac ttttaaaaca ccctttacct acacagaatt actttttaaa
     1501 tttccagtca agctgcgagt ttcaaaatta tagccggtag agaagacagt gctatttcaa
     1561 aagcaaacta acaagggtct taaattccaa aacaccaatc ctaacaagcc ttggactttt
     1621 gtaagtttag atcaaaggtg gcattgcatt caatgtcatg gtaagaagta ggtcgtctag
     1681 gtagaaatcc tcattcagcc ggtcaagtca gtacgagaaa ggtctcaatt tgaaattgtc
     1741 ttaaaaatat tttattgttt tgtactgtgg tgagtttaaa cgaaaaacac aaaaaaaaag
     1801 tgatacacag aaatcataaa aaattttaat acaaggtatt cgtacgtatc aaaaacattt
     1861 cggcacaatt ttttttctct gtactaaagt gttacgaaca ctacggtatt ttttagtgat
     1921 tttcaacgga caccgaaggt atataaacag cgttcgcgaa cggtcgcctt caaaaccaat
     1981 tgacatttgc agcagcaagt acaagtagaa agtaaagcgc aatcagcgaa aaatttatac
     2041 ttaattgttg gtgattaaag tacaattaaa agaacattct cgaaagtcac aagaaacgta
     2101 agtttttaac tcgctgttac caattagtaa taagagcaac aagacgttga gtaatttcaa
     2161 gaaaaactgc atttcaaggt ctttgttcgg ccattttttt tttattcaac gctctacgta
     2221 attacaaaat aagaaattgg cagccacgca tcttgttttc ccaatgaatt ggcatcaaaa
     2281 cgcaaacaaa tctataaata aaacttgcgt gttgattttc gccaagattt attggcaaat
     2341 tgtgaaattc gcagtgacgc atttgaaaat tcgagaaatc acgaacgcac atattgcatt
     2401 tgtgtgcatg ttattagtta gttgtttagt taattgaagt attttaccaa cgaaatccac
     2461 ttatttttag ctgaaataga gtaggttgct taaacaaagc cacgtctgaa aatttcttat
     2521 tgcttgtagt tgtgacgtca ccatatacac acaaaataat gtgtatgcat gcatttcagc
     2581 tgtgtatata tacatgcaca cactcgcaac acgaaaacga tgacgaagca acggaacaaa
     2641 ggtttctcaa ctaccctttg ttccctgttt cttcgctttc ctttgttcca atattcgtag
     2701 agggttaata ggggtttctc aacaaagttg gcgtcgataa ataagtttcc catttttatt
     2761 ccccagccag gaagttagtt tcaatagttt tgtaatttca acgaaactca tttgatttcg
     2821 tactaatttt ccacatctct attttgaccc gcagagcggc cgcatgactt cgaaagttta
     2881 tgatccagaa caaaggaaac ggatgataac tggtccgcag tggtgggcca gatgtaaaca
     2941 aatgaatgtt cttgattcat ttattaatta ttatgattca gaaaaacatg cagaaaatgc
     3001 tgttattttt ttacatggta acgcggcctc ttcttattta tggcgacatg ttgtgccaca
     3061 tattgagcca gtagcgcggt gtattatacc agaccttatt ggtatgggca aatcaggcaa
     3121 atctggtaat ggttcttata ggttacttga tcattacaaa tatcttactg catggtttga
     3181 acttcttaat ttaccaaaga agatcatttt tgtcggccat gattggggtg cttgtttggc
     3241 atttcattat agctatgagc atcaagataa gatcaaagca atagttcacg ctgaaagtgt
     3301 agtagatgtg attgaatcat gggatgaatg gcctgatatt gaagaagata ttgcgttgat
     3361 caaatctgaa gaaggagaaa aaatggtttt ggagaataac ttcttcgtgg aaaccatgtt
     3421 gccatcaaaa atcatgagaa agttagaacc agaagaattt gcagcatatc ttgaaccatt
     3481 caaagagaaa ggtgaagttc gtcgtccaac attatcatgg cctcgtgaaa tcccgttagt
     3541 aaaaggtggt aaacctgacg ttgtacaaat tgttaggaat tataatgctt atctacgtgc
     3601 aagtgatgat ttaccaaaaa tgtttattga atcggaccca ggattctttt ccaatgctat
     3661 tgttgaaggt gccaagaagt ttcctaatac tgaatttgtc aaagtaaaag gtcttcattt
     3721 ttcgcaagaa gatgcacctg atgaaatggg aaaatatatc aaatcgttcg ttgagcgagt
     3781 tctcaaaaat gaacaataag gcgcgccaca ttgatgagtt tggacaaacc acaactagaa
     3841 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     3901 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     3961 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     4021 attatgatca gtcgaccctt aagggccgtt aactcgaatc gctatccaag ccagctccat
     4081 tgtcggcatc gtgctcattc tattgacgct ggcagatttg gttttggcgc tatgggaccc
     4141 gttcggttac aacaacatgt ttccgcgcga gtttcccgac gacatgtcgc gcacgttcct
     4201 gactgcgtac tttgagagtt tcgacaacac cacgtccaga gaaatcatag agtttatgcc
     4261 cgagttcttt tcggaaatgg tcgaaacgga cgatgacgcc acgtttgaat ctctatttca
     4321 tttattagat tatgtggcat ctttagaagt taattccgac ggccaaatgt taaacttgga
     4381 ggagggtgat gaaattgagg attttgacga atctactttg gtggggcaag cgttagccac
     4441 tagctcgcta tacactcgca tggagtttat gcagtacacg tttaggcaaa acacactatt
     4501 gtctatgaac aaagaaaaca acaattttaa tcaaataatc atgggtttat ttgcaacaaa
     4561 cacaattgtg gcgtttacag catttgttat acacacagaa ctcatatttt ttatattttt
     4621 cgtaatcttc ctaatgatca cattttatta cataatcaaa gaatcgtacg aatattataa
     4681 aacaattgat ttgttatttt aaaaacgatt cattctaggc gatcgcttac ttgtacagct
     4741 cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc
     4801 gcttctcgtt ggggtctttg ctcagggcgg actgggtgct caggtagtgg ttgtcgggca
     4861 gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc
     4921 tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc ttctgcttgt
     4981 cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt
     5041 tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct
     5101 cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct
     5161 cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg
     5221 ggtagcggct gaagcactgc acgccgtagg tcagggtggt cacgagggtg ggccagggca
     5281 cgggcagctt gctggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc
     5341 cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca
     5401 ggatgggcac caccccggtg aacagctcct cgcccttgct caccataggg ccgggattct
     5461 cctccacgtc accgcatgtt agaagacttc ctctgccctc cacggcgatc tttccgccct
     5521 tcttggcctt tatgaggatc tctctgattt ttcttgcgtc gagttttccg gtaagacctt
     5581 tcggtacttc gtccacaaac acaactcctc cgcgcaactt tttcgcggtt gttacttgac
     5641 tggcgacgta atccacgatc tctttttccg tcatcgtctt tccgtgctcc aaaacaacaa
     5701 cggcggcggg aagttcaccg gcgtcatcgt cgggaagacc tgcgacacct gcgtcgaaga
     5761 tgttggggtg ttggagcaag atggattcca attcagcggg agccacctga tagcctttgt
     5821 acttaatcag agacttcagg cggtcaacga tgaagaagtg ttcgtcttcg tcccagtaag
     5881 ctatgtctcc agaatgtagc catccatcct tgtcaatcaa ggcgttggtc gcttccggat
     5941 tgtttacata accggacata atcataggac ctctcacaca cagttcgcct ctttgattaa
     6001 cgcccagcgt tttcccggta tccagatcca caaccttcgc ttcaaaaaat ggaacaactt
     6061 taccgaccgc gcccggttta tcatccccct cgggtgtaat cagaatagct gatgtagtct
     6121 cagtgagccc atatccttgc ctgatacctg gcagatggaa cctcttggca accgcttccc
     6181 cgacttcctt agagagggga gcgccaccag aagcaatttc gtgtaaatta gataaatcgt
     6241 atttgtcaat cagagtgctt ttggcgaaga aggagaatag ggttggcacc agcagcgcac
     6301 tttgaatctt gtaatcctga aggctcctca gaaacagctc ttcttcaaat ctatacatta
     6361 agacgactcg aaatccacat atcaaatatc cgagtgtagt aaacattcca aaaccgtgat
     6421 ggaatggaac aacacttaaa atcgcagtat ccggaatgat ttgattgcca aaaataggat
     6481 ctctggcatg cgagaatctc acgcaggcag ttctatgagg cagagcgaca cctttaggca
     6541 gaccagtaga tccagaggag ttcatgatca gtgcaattgt cttgtcccta tcgaaggact
     6601 ctggcacaaa atcgtattca ttaaaaccgg gaggtagatg agatgtgacg aacgtgtaca
     6661 tcgactgaaa tccctggtaa tccgttttag aatccatgat aataattttt tggatgattg
     6721 ggagcttttt ttgcacgttc aaaatttttt gcaacccctt tttggaaacg aacaccacgg
     6781 taggctgcga aatgcccata ctgttgagca attcacgttc attataaatg tcgttcgcgg
     6841 gcgcaactgc aactccgata aataacgcgc ccaacaccgg cataaagaat tgaagagagt
     6901 tttcactgca tacgacgatt ctgtgatttg tattcagccc atatcgtttc atagcttctg
     6961 ccaaccgaac ggacatttcg aagtactcag cgtaagtgat gtccacctcg atatgtgcat
     7021 ctgtaaaagc aattgttcca ggaaccaggg cgtatctctt catagcctta tgcagttgct
     7081 ctccagcggt tccatcttcc agcggataga atggcgccgg gcctttcttt atgtttttgg
     7141 cgtcttccat cctaggcaat tctgatcccc gggcgggtac caatgaacag gacctaacgc
     7201 acagtcacgt tattgtttac ataaatgatt ttttttacta ttcaaactta ctctgtttgt
     7261 gtactcccac tggtatagcc ttcttttatc ttttctggtt caggctctat caccggtttt
     7321 caaaaaaaaa ttcgtccgca cacaaccttt cctctcaaca agcaaacgtg cactgaattt
     7381 aagtgtatac ttcggtaagc tacggaggcc tggcagattg tttagcttgt tcagctgcgc
     7441 ttgtttattt gcttagcttt cgcttagcga cgtgttcact ttgcttgttt gaattgaatt
     7501 gtcgctccgt agacgaagcg cctctattta tactccggcg ctccttaatt aaatagatct
     7561 aagtcgcagt atttacaaac aaccatgaat gtaagtatat tcgaagaata tacttacatt
     7621 catggttgtt tgtaaatact gctgggatcc atgcgtactc gagccgccat ttaaatattg
     7681 cccaattccc aattcccgga tccgctagca taacttcgta tagcatacat tatacgaagt
     7741 tatgctagcc tagttccagt gaaatccaag cattttctaa attaaatgta ttcttattat
     7801 tatagttgtt atttttgata tatataaaca acactattat gcccaccatt tttttgagat
     7861 gcatctacac aaggaacaaa cactggatgt cactttcagt tcaaattgta acgctaatca
     7921 ctccgaacag gtcacaaaaa attaccttaa aaagtcataa tattaaatta gaataaatat
     7981 agctgtgagg gaaatatata caaatatatt ggagcaaata aattgtacat acaaatattt
     8041 attactaatt tctattgaga cgaaatgaac cactcggaac catttgagcg aaccgaatcg
     8101 cgcggaacta acgacagtcg ctccaaggtc gtcgaacaaa aggtgaatgt gttgcggaga
     8161 gcgggtggga gacagcgaaa gagcaactac gaaacgtggt gtggtggagg tgaattatga
     8221 agagggcgcg cgatttgaaa agtatgtata taaaaaatat atcccggtgt tttatgtagc
     8281 gataaacgag tttttgatgt aaggtatgca ggtgtgtaag tcttttggtt agaagacaaa
     8341 tccaaagtct acttgtgggg atgttcgaag gggaaatact tgtattctat aggtcatatc
     8401 ttgtttttat tggcacaaat ataattacat tagctttttg agggggcaat aaacagtaaa
     8461 cacgatggta ataatggtaa aaaaaaaaaa caagcagtta tttcggatat atgtcggcta
     8521 ctccttgcgt cgggcccgaa gtcttagagc cagatatgcg agcacccgga agctcacgat
     8581 gagaatggcc agacccacgt agtccagcgg cagatcggcg gcggagaagt taagcgtctc
     8641 caggatgacc ttgcccgaac tggggcacgt ggtgttcgac gatgtgcagc taatttcgcc
     8701 cggctccacg tccgcccatt ggttaatcag cagaccctcg ttggcgtaac ggaaccatga
     8761 gaggtacgac aaccatttga ggtatactgg caccgagccc gagttcaaga agaagccgcc
     8821 aaagagcagg aatggtatga taaccggcgg acccacagac agcgccatcg aggtcgagga
     8881 gctggcgcag gatattagat atccgaagga cgttgacaca ttggccacca gagtgaccag
     8941 cgccaggcag ttgaagaagt gcagcactcc ggcccgcagt ccgatcatcg gataggcaat
     9001 cgccgtgaag accagtggca ctgtgagaaa aagcggcaat tcggcaatcg ttttgcccag
     9061 aaagtatgtg tcacagcgat aaagtcgact tcgggcctcc ctcataaaaa ctggcagctc
     9121 tgaggtgaac acctaaatcg aatcgattca ttagaaagtt agtaaattat tgaaatgcaa
     9181 atgtattcta aacatgactt acatttatcg tggcaaagac gttttgaaag gtcatgttgg
     9241 tcaggaagag gaagatggct ccgttgatat tcatcacacc cacttgcgtg agttgttggc
     9301 ccaaaaagat gaggccaatc aagatggcaa ccatctgcaa attaaaatgt tactcgcatc
     9361 tcattaatat tcgcgagtta aatgaaattt atttatcttc tgcaaaacta taaactatac
     9421 atctcattga aaaaaactaa gaagggtgtg gaatcaggca attctatcta aaatctagcg
     9481 aatttgtttc caagaattgt aagcgttata tcatttgttt ccactggaac cactcaccgt
     9541 tgtctgaata agtcgcactt ttacgaggag tggttccttg agcaccgaca gccaggatcg
     9601 ccacaggacc gcccggaact gcatgaacca ggtggccttg taggtgtacc cattctccgg
     9661 ctgctccagt ggcttctcca gatttttggt ggccaacaac tgctccatat cccgggctac
     9721 tttgctaatg gcaaaattgt cgcatatctt ggcgatccga tcacgggact cgatctcccg
     9781 tccgggcaca acggccaaca cctgtacgta aaagtccgcc ggattgtagt tggtaggaca
     9841 ctgggcaccc acgctggata ggagttgaga tgttatgtaa tactagatac ccttaataaa
     9901 cacatcgaac tcactaggaa aagaagtcga cggcttcgct gggagtgccc aagaaagcta
     9961 ccctgccctc ggccatcaga aggatcttgt caaagagctc aaacagctcg gaagacggct
    10021 gatgaatggt caggatgacg gtcttgccct tctgcgacag cttcttcagc acctggacga
    10081 cgctgtgggc ggtaaaggag tccagtccgg aggtgggctc atcgcagatc agaagcggcg
    10141 gatcggttag agcctcggag gcgaatgcca gacgcttcct ttctccgccg gacagacctt
    10201 tcaccctgcc gggcacaccg atgatcgtgt gctgacattt gctgagcgaa agctcctgga
    10261 tcacctgatc cacgcgggcc actcgctgcc gataggtcag atgtcgtggc atccgcacca
    10321 tggcttggaa aatcaggtgt tccctggccg ttagggagcc gataaagagg tcatcctgct
    10381 ggacataggc gcacctggcc tgcatctcct tggcgtccac aggttggcca ttgagcagtc
    10441 gcatcccgga tggcgatact tggatgccct gcggcgatcg aaaggcaagg gcattcagca
    10501 gggtcgtctt tccggcaccg gaactgccca tcacggccaa aagttcgccc ggataggcca
    10561 cgccgcaaac tgagtttcaa attggtaatt ggacccttta ttaagatttc acacagatca
    10621 gccgactgcg aatagaaact caccgttctt gagcaaatgt ttcctgggcg ccggtatgtg
    10681 tcgctcgttg cagaatagtc cgcgtgtccg gttgaccagc tgccgccatc cggagcccgg
    10741 ctgattgacc gccccaaaga tgtccatatt gtgccaggca taggtgaggt tctcggctag
    10801 ttggccgctc cctgaaccgg agtcctccgg cggactgggt ggccggagcg tgccgtagtt
    10861 tttggcctgc ccgaagccct ggttaatgca gctctgcgaa gccgctccgc tgtcaccctg
    10921 caatgatagg ggatctcaaa tatcaactac aagcgttatg ctcatctaac cccgaacaaa
    10981 aagtaccccg aagtatccta cgaagtaggt ttatactttt atttattttt tgtgcatcta
    11041 ggatcagctt aaaatatctg gttgttatat tttttgtaaa aaagaatata gtcgaaaatg
    11101 aatgccttta gatgtcttga tcatgatatg atctcaaaaa ttgtcttata tagcgagaac
    11161 agctaccaga ataatctgtt tcgtgtcact atttgtttgt gcaattgcgg tttgggattt
    11221 ttgtgggtcg cagttctcac gccgcagaca atttgatgtt gcaatcgcag ttcctataga
    11281 tcaagtgaac ttaagatgta tgcacatgta ctactcacat tgttcagatg ctcggcagat
    11341 gggtgtttgc tgcctccgcg aattaatagc tcctgatcct cttggcccat tgccgggatt
    11401 tttcacactt tcccctgctt acccacccaa aaccaatcac caccccaatc actcaaaaaa
    11461 caaacaaaaa taagaagcga gaggagtttt ggcacagcac tttgtgttta attgatggcg
    11521 taaaccgctt ggagcttcgt cacgaaaccg ctgacaaaat gcaactgaag gcggacattg
    11581 acgctacgta acgctacaaa cggtggcgaa agagatagcg gacgcagcgg cgaaagagac
    11641 ggcgatattt ctgtggacag agaaggaggc aaacagcgct gactttgagt ggaatgtcat
    11701 tttgagtgag aggtaatcga aagaacctgg tacatcaaat acccttggat cgaagtaaat
    11761 ttaaaactga tcagataagt tcaatgatat ccagtgcagt aaaaaaaaaa aatgtttttt
    11821 ttatctactt tccgcaaaaa tgggttttat taacttacat acatactagg ggcccggtac
    11881 gtacccaatt cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt
    11941 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
    12001 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
    12061 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
    12121 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
    12181 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
    12241 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
    12301 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
    12361 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
    12421 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
    12481 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
    12541 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
    12601 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
    12661 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
    12721 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
    12781 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
    12841 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
    12901 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
    12961 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
    13021 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    13081 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    13141 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    13201 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    13261 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    13321 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    13381 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    13441 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    13501 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    13561 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    13621 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    13681 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    13741 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    13801 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    13861 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    13921 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    13981 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    14041 tggagcgaac gacctacacc
//
