LOCUS       sequence-31601-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UBE2G1
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..88
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             577..594
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(647..665)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      661..708
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(832..851)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca
       61 cagcagcggc ctggttccgc gtggtagtct gctactgcga agacagctgg cagaactcaa
      121 caaaaatcca gtggaaggct tttctgcagg tttaatagat gacaatgatc tctaccgatg
      181 ggaagtcctt attattggcc ctccagatac actttatgaa ggtggtgttt ttaaggctca
      241 tcttactttc ccaaaagatt atcccctccg acctcctaaa atgaaattca ttacagaaat
      301 ctggcaccca aatgttgata aaaatggtga tgtgtgcatt tctattcttc atgagcctgg
      361 ggaagataag tatggttatg aaaagccaga ggaacgctgg ctccctatcc acactgtgga
      421 aaccatcatg attagtgtca tttctatgct ggcagaccct aatggagact cacctgctaa
      481 tgttgatgct gcgaaagaat ggagggaaga tagaaatgga gaatttaaaa gaaaagttgc
      541 ccgctgttga tgacgaagct tgcggccgca ctcgagcacc accaccacca ccactgagat
      601 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
      661 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
      721 actatatccg gattggcgaa tggnacgcgc cctgtagcgg cgcattaagc gcggcgggtg
      781 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
      841 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
      901 ggctcccttt a
//
