LOCUS       sequence-316011-       14055 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PipO(3x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3'UTR-Ubiquitin-Renil
            la-attB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14055)
  AUTHORS   Gamez S, Vesga LC, Mendez-Sanchez SC, Akbari OS
  TITLE     Spatial control of gene expression in flies using bacterially 
            derived binary transactivation systems.
  JOURNAL   Insect Mol Biol. 2021 Oct;30(5):461-471. doi: 10.1111/imb.12717. 
            Epub 2021 May 24.
  PUBMED    33963794
REFERENCE   2  (bases 1 to 14055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/imb.12717";
            journalName: "Insect Mol Biol"; date: "2021-10"; volume: "30"; 
            issue: "5"; pages: "461-471"
COMMENT     Sequence Label: 908G
FEATURES             Location/Qualifiers
     source          1..14055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     621..640
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        621..639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(673..691)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1143..1214,1616..1889,1964..2618,2680..2995,3199..3330,
                     3401..4015)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4790..4823
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5378..7027
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(5444..5462)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             7028..7081
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(7082..7084,7085..7087,7088..7801)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTSKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7127..7148)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7388..7407)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7735..7756
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8605..8686
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8642..8661)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8696..8715
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(8729..9664)
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     protein_bind    11577..11646
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(11865..11883)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11904..11920)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11904..11920)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11917..11939)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11928..11944
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11952..11958,11959..11976,11977..11982))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11997..12018
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12135..12152)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12306..12894)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12386..12405)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13065..13856,13857..13925))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13688..13707
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13926..14030)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggt acgtaccggg
      661 cccctagtat gtatgtaagt taataaaacc catttttgcg gaaagtagat aaaaaaaaca
      721 tttttttttt ttactgcact ggatatcatt gaacttatct gatcagtttt aaatttactt
      781 cgatccaagg gtatttgatg taccaggttc tttcgattac ctctcactca aaatgacatt
      841 ccactcaaag tcagcgctgt ttgcctcctt ctctgtccac agaaatatcg ccgtctcttt
      901 cgccgctgcg tccgctatct ctttcgccac cgtttgtagc gttacgtagc gtcaatgtcc
      961 gccttcagtt gcattttgtc agcggtttcg tgacgaagct ccaagcggtt tacgccatca
     1021 attaaacaca aagtgctgtg ccaaaactcc tctcgcttct tatttttgtt tgttttttga
     1081 gtgattgggg tggtgattgg ttttgggtgg gtaagcaggg gaaagtgtga aaaatcccgg
     1141 caatgggcca agaggatcag gagctattaa ttcgcggagg cagcaaacac ccatctgccg
     1201 agcatctgaa caatgtgagt agtacatgtg catacatctt aagttcactt gatctatagg
     1261 aactgcgatt gcaacatcaa attgtctgcg gcgtgagaac tgcgacccac aaaaatccca
     1321 aaccgcaatt gcacaaacaa atagtgacac gaaacagatt attctggtag ctgttctcgc
     1381 tatataagac aatttttgag atcatatcat gatcaagaca tctaaaggca ttcattttcg
     1441 actatattct tttttacaaa aaatataaca accagatatt ttaagctgat cctagatgca
     1501 caaaaaataa ataaaagtat aaacctactt cgtaggatac ttcggggtac tttttgttcg
     1561 gggttagatg agcataacgc ttgtagttga tatttgagat cccctatcat tgcagggtga
     1621 cagcggagcg gcttcgcaga gctgcattaa ccagggcttc gggcaggcca aaaactacgg
     1681 cacgctccgg ccacccagtc cgccggagga ctccggttca gggagcggcc aactagccga
     1741 gaacctcacc tatgcctggc acaatatgga catctttggg gcggtcaatc agccgggctc
     1801 cggatggcgg cagctggtca accggacacg cggactattc tgcaacgagc gacacatacc
     1861 ggcgcccagg aaacatttgc tcaagaacgg tgagtttcta ttcgcagtcg gctgatctgt
     1921 gtgaaatctt aataaagggt ccaattacca atttgaaact cagtttgcgg cgtggcctat
     1981 ccgggcgaac ttttggccgt gatgggcagt tccggtgccg gaaagacgac cctgctgaat
     2041 gcccttgcct ttcgatcgcc gcagggcatc caagtatcgc catccgggat gcgactgctc
     2101 aatggccaac ctgtggacgc caaggagatg caggccaggt gcgcctatgt ccagcaggat
     2161 gacctcttta tcggctccct aacggccagg gaacacctga ttttccaagc catggtgcgg
     2221 atgccacgac atctgaccta tcggcagcga gtggcccgcg tggatcaggt gatccaggag
     2281 ctttcgctca gcaaatgtca gcacacgatc atcggtgtgc ccggcagggt gaaaggtctg
     2341 tccggcggag aaaggaagcg tctggcattc gcctccgagg ctctaaccga tccgccgctt
     2401 ctgatctgcg atgagcccac ctccggactg gactccttta ccgcccacag cgtcgtccag
     2461 gtgctgaaga agctgtcgca gaagggcaag accgtcatcc tgaccattca tcagccgtct
     2521 tccgagctgt ttgagctctt tgacaagatc cttctgatgg ccgagggcag ggtagctttc
     2581 ttgggcactc ccagcgaagc cgtcgacttc ttttcctagt gagttcgatg tgtttattaa
     2641 gggtatctag tattacataa catctcaact cctatccagc gtgggtgccc agtgtcctac
     2701 caactacaat ccggcggact tttacgtaca ggtgttggcc gttgtgcccg gacgggagat
     2761 cgagtcccgt gatcggatcg ccaagatatg cgacaatttt gccattagca aagtagcccg
     2821 ggatatggag cagttgttgg ccaccaaaaa tctggagaag ccactggagc agccggagaa
     2881 tgggtacacc tacaaggcca cctggttcat gcagttccgg gcggtcctgt ggcgatcctg
     2941 gctgtcggtg ctcaaggaac cactcctcgt aaaagtgcga cttattcaga caacggtgag
     3001 tggttccagt ggaaacaaat gatataacgc ttacaattct tggaaacaaa ttcgctagat
     3061 tttagataga attgcctgat tccacaccct tcttagtttt tttcaatgag atgtatagtt
     3121 tatagttttg cagaagataa ataaatttca tttaactcgc gaatattaat gagatgcgag
     3181 taacatttta atttgcagat ggttgccatc ttgattggcc tcatcttttt gggccaacaa
     3241 ctcacgcaag tgggtgtgat gaatatcaac ggagccatct tcctcttcct gaccaacatg
     3301 acctttcaaa acgtctttgc cacgataaat gtaagtcatg tttagaatac atttgcattt
     3361 caataattta ctaactttct aatgaatcga ttcgatttag gtgttcacct cagagctgcc
     3421 agtttttatg agggaggccc gaagtcgact ttatcgctgt gacacatact ttctgggcaa
     3481 aacgattgcc gaattgccgc tttttctcac agtgccactg gtcttcacgg cgattgccta
     3541 tccgatgatc ggactgcggg ccggagtgct gcacttcttc aactgcctgg cgctggtcac
     3601 tctggtggcc aatgtgtcaa cgtccttcgg atatctaata tcctgcgcca gctcctcgac
     3661 ctcgatggcg ctgtctgtgg gtccgccggt tatcatacca ttcctgctct ttggcggctt
     3721 cttcttgaac tcgggctcgg tgccagtata cctcaaatgg ttgtcgtacc tctcatggtt
     3781 ccgttacgcc aacgagggtc tgctgattaa ccaatgggcg gacgtggagc cgggcgaaat
     3841 tagctgcaca tcgtcgaaca ccacgtgccc cagttcgggc aaggtcatcc tggagacgct
     3901 taacttctcc gccgccgatc tgccgctgga ctacgtgggt ctggccattc tcatcgtgag
     3961 cttccgggtg ctcgcatatc tggctctaag acttcgggcc cgacgcaagg agtagccgac
     4021 atatatccga aataactgct tgtttttttt ttttaccatt attaccatcg tgtttactgt
     4081 ttattgcccc ctcaaaaagc taatgtaatt atatttgtgc caataaaaac aagatatgac
     4141 ctatagaata caagtatttc cccttcgaac atccccacaa gtagactttg gatttgtctt
     4201 ctaaccaaaa gacttacaca cctgcatacc ttacatcaaa aactcgttta tcgctacata
     4261 aaacaccggg atatattttt tatatacata cttttcaaat cgcgcgccct cttcataatt
     4321 cacctccacc acaccacgtt tcgtagttgc tctttcgctg tctcccaccc gctctccgca
     4381 acacattcac cttttgttcg acgaccttgg agcgactgtc gttagttccg cgcgattcgg
     4441 ttcgctcaaa tggttccgag tggttcattt cgtctcaata gaaattagta ataaatattt
     4501 gtatgtacaa tttatttgct ccaatatatt tgtatatatt tccctcacag ctatatttat
     4561 tctaatttaa tattatgact ttttaaggta attttttgtg acctgttcgg agtgattagc
     4621 gttacaattt gaactgaaag tgacatccag tgtttgttcc ttgtgtagat gcatctcaaa
     4681 aaaatggtgg gcataatagt gttgtttata tatatcaaaa ataacaacta taataataag
     4741 aatacattta atttagaaaa tgcttggatt tcactggaac taggctagca taacttcgta
     4801 taatgtatgc tatacgaagt tatgctagcg gatccgggaa ttgggaattg ggcaatattt
     4861 aaatggcggc tcgaggcgct attttcattc aggctccttg tacggtgtac gggaagatac
     4921 tcgtacaccg tacaagagat tcccatacgc tgtacagcgc tatttggatc catgcggccc
     4981 tgcagagcgc cggagtataa atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa
     5041 caagcaaagt gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca
     5101 agctaaacaa tctgccaggc ctccgtagct taccgaagta tacacttaaa ttcagtgcac
     5161 gtttgcttgt tgagaggaaa ggttgtgtgc ggacgaattt ttttttgaaa accggtgata
     5221 gagcctgaac cagaaaagat aaaagaaggc tataccagtg ggagtacaca aacagagtaa
     5281 gtttgaatag taaaaaaaat catttatgta aacaataacg tgactgtgcg ttaggtcctg
     5341 ttcattggta cccgcccggg gatcagaatt gcctaggatg gaagacgcca aaaacataaa
     5401 gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa
     5461 ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga
     5521 ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa
     5581 acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt
     5641 ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat
     5701 ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt
     5761 ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa
     5821 aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt
     5881 cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag
     5941 ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt
     6001 cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg
     6061 caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg
     6121 aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt
     6181 tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt
     6241 gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa
     6301 tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc
     6361 caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc
     6421 tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt
     6481 ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg
     6541 cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac
     6601 caacgccttg attgacaagg atggatggct acattctgga gacatagctt actgggacga
     6661 agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca
     6721 ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg
     6781 tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga
     6841 gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac
     6901 cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg
     6961 aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat
     7021 cgccgtggag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc
     7081 tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     7141 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     7201 cggcaagctg accctgaagt tcatctgcac caccagcaag ctgcccgtgc cctggcccac
     7261 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     7321 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     7381 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     7441 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     7501 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     7561 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     7621 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     7681 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     7741 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     7801 agcgatcgcc tagaatgaat cgtttttaaa ataacaaatc aattgtttta taatattcgt
     7861 acgattcttt gattatgtaa taaaatgtga tcattaggaa gattacgaaa aatataaaaa
     7921 atatgagttc tgtgtgtata acaaatgctg taaacgccac aattgtgttt gttgcaaata
     7981 aacccatgat tatttgatta aaattgttgt tttctttgtt catagacaat agtgtgtttt
     8041 gcctaaacgt gtactgcata aactccatgc gagtgtatag cgagctagtg gctaacgctt
     8101 gccccaccaa agtagattcg tcaaaatcct caatttcatc accctcctcc aagtttaaca
     8161 tttggccgtc ggaattaact tctaaagatg ccacataatc taataaatga aatagagatt
     8221 caaacgtggc gtcatcgtcc gtttcgacca tttccgaaaa gaactcgggc ataaactcta
     8281 tgatttctct ggacgtggtg ttgtcgaaac tctcaaagta cgcagtcagg aacgtgcgcg
     8341 acatgtcgtc gggaaactcg cgcggaaaca tgttgttgta accgaacggg tcccatagcg
     8401 ccaaaaccaa atctgccagc gtcaatagaa tgagcacgat gccgacaatg gagctggctt
     8461 ggatagcgat tcgagttaac ggcccttaag ggtcgactga tcataatcag ccataccaca
     8521 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     8581 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     8641 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8701 ttgtccaaac tcatcaatgt ggcgcgcctt attgttcatt tttgagaact cgctcaacga
     8761 acgatttgat atattttccc atttcatcag gtgcatcttc ttgcgaaaaa tgaagacctt
     8821 ttactttgac aaattcagta ttaggaaact tcttggcacc ttcaacaata gcattggaaa
     8881 agaatcctgg gtccgattca ataaacattt ttggtaaatc atcacttgca cgtagataag
     8941 cattataatt cctaacaatt tgtacaacgt caggtttacc accttttact aacgggattt
     9001 cacgaggcca tgataatgtt ggacgacgaa cttcaccttt ctctttgaat ggttcaagat
     9061 atgctgcaaa ttcttctggt tctaactttc tcatgatttt tgatggcaac atggtttcca
     9121 cgaagaagtt attctccaaa accatttttt ctccttcttc agatttgatc aacgcaatat
     9181 cttcttcaat atcaggccat tcatcccatg attcaatcac atctactaca ctttcagcgt
     9241 gaactattgc tttgatctta tcttgatgct catagctata atgaaatgcc aaacaagcac
     9301 cccaatcatg gccgacaaaa atgatcttct ttggtaaatt aagaagttca aaccatgcag
     9361 taagatattt gtaatgatca agtaacctat aagaaccatt accagatttg cctgatttgc
     9421 ccataccaat aaggtctggt ataatacacc gcgctactgg ctcaatatgt ggcacaacat
     9481 gtcgccataa ataagaagag gccgcgttac catgtaaaaa aataacagca ttttctgcat
     9541 gtttttctga atcataataa ttaataaatg aatcaagaac attcatttgt ttacatctgg
     9601 cccaccactg cggaccagtt atcatccgtt tcctttgttc tggatcataa actttcgaag
     9661 tcatgcggcc gctctgcggg tcaaaataga gatgtggaaa attagtacga aatcaaatga
     9721 gtttcgttga aattacaaaa ctattgaaac taacttcctg gctggggaat aaaaatggga
     9781 aacttattta tcgacgccaa ctttgttgag aaacccctat taaccctcta cgaatattgg
     9841 aacaaaggaa agcgaagaaa cagggaacaa agggtagttg agaaaccttt gttccgttgc
     9901 ttcgtcatcg ttttcgtgtt gcgagtgtgt gcatgtatat atacacagct gaaatgcatg
     9961 catacacatt attttgtgtg tatatggtga cgtcacaact acaagcaata agaaattttc
    10021 agacgtggct ttgtttaagc aacctactct atttcagcta aaaataagtg gatttcgttg
    10081 gtaaaatact tcaattaact aaacaactaa ctaataacat gcacacaaat gcaatatgtg
    10141 cgttcgtgat ttctcgaatt ttcaaatgcg tcactgcgaa tttcacaatt tgccaataaa
    10201 tcttggcgaa aatcaacacg caagttttat ttatagattt gtttgcgttt tgatgccaat
    10261 tcattgggaa aacaagatgc gtggctgcca atttcttatt ttgtaattac gtagagcgtt
    10321 gaataaaaaa aaaatggccg aacaaagacc ttgaaatgca gtttttcttg aaattactca
    10381 acgtcttgtt gctcttatta ctaattggta acagcgagtt aaaaacttac gtttcttgtg
    10441 actttcgaga atgttctttt aattgtactt taatcaccaa caattaagta taaatttttc
    10501 gctgattgcg ctttactttc tacttgtact tgctgctgca aatgtcaatt ggttttgaag
    10561 gcgaccgttc gcgaacgctg tttatatacc ttcggtgtcc gttgaaaatc actaaaaaat
    10621 accgtagtgt tcgtaacact ttagtacaga gaaaaaaaat tgtgccgaaa tgtttttgat
    10681 acgtacgaat accttgtatt aaaatttttt atgatttctg tgtatcactt tttttttgtg
    10741 tttttcgttt aaactcacca cagtacaaaa caataaaata tttttaagac aatttcaaat
    10801 tgagaccttt ctcgtactga cttgaccggc tgaatgagga tttctaccta gacgacctac
    10861 ttcttaccat gacattgaat gcaatgccac ctttgatcta aacttacaaa agtccaaggc
    10921 ttgttaggat tggtgttttg gaatttaaga cccttgttag tttgcttttg aaatagcact
    10981 gtcttctcta ccggctataa ttttgaaact cgcagcttga ctggaaattt aaaaagtaat
    11041 tctgtgtagg taaagggtgt tttaaaagtg tgatgtgttg agcgttgcgg caacgactgc
    11101 tatttatgta tatattttca aaacttattg tttttgaagt gttttaaatg gagctatctg
    11161 gcaacgctgc gcataatctt acacaagctt ttcttaatcc atttttaagt gaaatttgtt
    11221 tatactcttt cggcaaataa ttgttaaatc gctttaagtg ggcttacatc tggataagta
    11281 atgaaaacct gcatattata atattaaaac atataatcca ctgtgctttc cccgtgtgtg
    11341 gccatatacc taaaaaagtt tattttcgca gagccccgca cggtcacact acggttcggc
    11401 gattttcgat tttggacagt actgattgca agcgcaccga aagcaaaatg gagctggaga
    11461 ttttgaacgc gaagaacagc aagccgtacg gcaaggtgaa ggtgccctcc ggcgccacgc
    11521 ccatcggcga tctgcgcgtc tagagatcca ctagtgtcga cgatgtaggt cacggtctcg
    11581 aagccgcggt gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta ctccacctca
    11641 cccatctggt ccatcatgat gaacgggtcg aggtggcggt agttgatccc ggcgaacgcg
    11701 cggcgcaccg ggaagccctc gccctcgaaa ccgctgggcg cggtggtcac ggtgagcacg
    11761 ggacgtgcga cggcgtcggc gggtgcggat acgcggggca gcgtcagcgg gttctcgacg
    11821 gtcacggcgg gcatgtcgac actagttcta gccagctttt gttcccttta gtgagggtta
    11881 atttcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
    11941 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
    12001 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    12061 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
    12121 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    12181 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    12241 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    12301 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    12361 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    12421 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    12481 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    12541 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    12601 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    12661 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    12721 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
    12781 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    12841 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    12901 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    12961 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    13021 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    13081 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    13141 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    13201 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    13261 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    13321 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    13381 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    13441 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    13501 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    13561 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    13621 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    13681 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    13741 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    13801 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    13861 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    13921 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    13981 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    14041 cgaaaagtgc cacct
//
