LOCUS       sequence-316017-       14010 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TetO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3'UTR-Ubiquitin-Renil
            la-attB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14010)
  AUTHORS   Gamez S, Vesga LC, Mendez-Sanchez SC, Akbari OS
  TITLE     Spatial control of gene expression in flies using bacterially 
            derived binary transactivation systems.
  JOURNAL   Insect Mol Biol. 2021 Oct;30(5):461-471. doi: 10.1111/imb.12717. 
            Epub 2021 May 24.
  PUBMED    33963794
REFERENCE   2  (bases 1 to 14010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/imb.12717";
            journalName: "Insect Mol Biol"; date: "2021-10"; volume: "30"; 
            issue: "5"; pages: "461-471"
COMMENT     Sequence Label: 908H
FEATURES             Location/Qualifiers
     source          1..14010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     621..640
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        621..639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(673..691)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1143..1214,1616..1889,1964..2618,2680..2995,3199..3330,
                     3401..4015)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4790..4823
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4876..4894
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4912..4930
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             5333..6982
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(5399..5417)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             6983..7036
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(7037..7039,7040..7042,7043..7756)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTSKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7082..7103)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7343..7362)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7690..7711
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8560..8641
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8597..8616)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8651..8670
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(8684..9619)
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     protein_bind    11532..11601
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(11820..11838)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11859..11875)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11859..11875)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11872..11894)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11883..11899
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11907..11913,11914..11931,11932..11937))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11952..11973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12090..12107)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12261..12849)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12341..12360)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13020..13811,13812..13880))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13643..13662
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13881..13985)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggt acgtaccggg
      661 cccctagtat gtatgtaagt taataaaacc catttttgcg gaaagtagat aaaaaaaaca
      721 tttttttttt ttactgcact ggatatcatt gaacttatct gatcagtttt aaatttactt
      781 cgatccaagg gtatttgatg taccaggttc tttcgattac ctctcactca aaatgacatt
      841 ccactcaaag tcagcgctgt ttgcctcctt ctctgtccac agaaatatcg ccgtctcttt
      901 cgccgctgcg tccgctatct ctttcgccac cgtttgtagc gttacgtagc gtcaatgtcc
      961 gccttcagtt gcattttgtc agcggtttcg tgacgaagct ccaagcggtt tacgccatca
     1021 attaaacaca aagtgctgtg ccaaaactcc tctcgcttct tatttttgtt tgttttttga
     1081 gtgattgggg tggtgattgg ttttgggtgg gtaagcaggg gaaagtgtga aaaatcccgg
     1141 caatgggcca agaggatcag gagctattaa ttcgcggagg cagcaaacac ccatctgccg
     1201 agcatctgaa caatgtgagt agtacatgtg catacatctt aagttcactt gatctatagg
     1261 aactgcgatt gcaacatcaa attgtctgcg gcgtgagaac tgcgacccac aaaaatccca
     1321 aaccgcaatt gcacaaacaa atagtgacac gaaacagatt attctggtag ctgttctcgc
     1381 tatataagac aatttttgag atcatatcat gatcaagaca tctaaaggca ttcattttcg
     1441 actatattct tttttacaaa aaatataaca accagatatt ttaagctgat cctagatgca
     1501 caaaaaataa ataaaagtat aaacctactt cgtaggatac ttcggggtac tttttgttcg
     1561 gggttagatg agcataacgc ttgtagttga tatttgagat cccctatcat tgcagggtga
     1621 cagcggagcg gcttcgcaga gctgcattaa ccagggcttc gggcaggcca aaaactacgg
     1681 cacgctccgg ccacccagtc cgccggagga ctccggttca gggagcggcc aactagccga
     1741 gaacctcacc tatgcctggc acaatatgga catctttggg gcggtcaatc agccgggctc
     1801 cggatggcgg cagctggtca accggacacg cggactattc tgcaacgagc gacacatacc
     1861 ggcgcccagg aaacatttgc tcaagaacgg tgagtttcta ttcgcagtcg gctgatctgt
     1921 gtgaaatctt aataaagggt ccaattacca atttgaaact cagtttgcgg cgtggcctat
     1981 ccgggcgaac ttttggccgt gatgggcagt tccggtgccg gaaagacgac cctgctgaat
     2041 gcccttgcct ttcgatcgcc gcagggcatc caagtatcgc catccgggat gcgactgctc
     2101 aatggccaac ctgtggacgc caaggagatg caggccaggt gcgcctatgt ccagcaggat
     2161 gacctcttta tcggctccct aacggccagg gaacacctga ttttccaagc catggtgcgg
     2221 atgccacgac atctgaccta tcggcagcga gtggcccgcg tggatcaggt gatccaggag
     2281 ctttcgctca gcaaatgtca gcacacgatc atcggtgtgc ccggcagggt gaaaggtctg
     2341 tccggcggag aaaggaagcg tctggcattc gcctccgagg ctctaaccga tccgccgctt
     2401 ctgatctgcg atgagcccac ctccggactg gactccttta ccgcccacag cgtcgtccag
     2461 gtgctgaaga agctgtcgca gaagggcaag accgtcatcc tgaccattca tcagccgtct
     2521 tccgagctgt ttgagctctt tgacaagatc cttctgatgg ccgagggcag ggtagctttc
     2581 ttgggcactc ccagcgaagc cgtcgacttc ttttcctagt gagttcgatg tgtttattaa
     2641 gggtatctag tattacataa catctcaact cctatccagc gtgggtgccc agtgtcctac
     2701 caactacaat ccggcggact tttacgtaca ggtgttggcc gttgtgcccg gacgggagat
     2761 cgagtcccgt gatcggatcg ccaagatatg cgacaatttt gccattagca aagtagcccg
     2821 ggatatggag cagttgttgg ccaccaaaaa tctggagaag ccactggagc agccggagaa
     2881 tgggtacacc tacaaggcca cctggttcat gcagttccgg gcggtcctgt ggcgatcctg
     2941 gctgtcggtg ctcaaggaac cactcctcgt aaaagtgcga cttattcaga caacggtgag
     3001 tggttccagt ggaaacaaat gatataacgc ttacaattct tggaaacaaa ttcgctagat
     3061 tttagataga attgcctgat tccacaccct tcttagtttt tttcaatgag atgtatagtt
     3121 tatagttttg cagaagataa ataaatttca tttaactcgc gaatattaat gagatgcgag
     3181 taacatttta atttgcagat ggttgccatc ttgattggcc tcatcttttt gggccaacaa
     3241 ctcacgcaag tgggtgtgat gaatatcaac ggagccatct tcctcttcct gaccaacatg
     3301 acctttcaaa acgtctttgc cacgataaat gtaagtcatg tttagaatac atttgcattt
     3361 caataattta ctaactttct aatgaatcga ttcgatttag gtgttcacct cagagctgcc
     3421 agtttttatg agggaggccc gaagtcgact ttatcgctgt gacacatact ttctgggcaa
     3481 aacgattgcc gaattgccgc tttttctcac agtgccactg gtcttcacgg cgattgccta
     3541 tccgatgatc ggactgcggg ccggagtgct gcacttcttc aactgcctgg cgctggtcac
     3601 tctggtggcc aatgtgtcaa cgtccttcgg atatctaata tcctgcgcca gctcctcgac
     3661 ctcgatggcg ctgtctgtgg gtccgccggt tatcatacca ttcctgctct ttggcggctt
     3721 cttcttgaac tcgggctcgg tgccagtata cctcaaatgg ttgtcgtacc tctcatggtt
     3781 ccgttacgcc aacgagggtc tgctgattaa ccaatgggcg gacgtggagc cgggcgaaat
     3841 tagctgcaca tcgtcgaaca ccacgtgccc cagttcgggc aaggtcatcc tggagacgct
     3901 taacttctcc gccgccgatc tgccgctgga ctacgtgggt ctggccattc tcatcgtgag
     3961 cttccgggtg ctcgcatatc tggctctaag acttcgggcc cgacgcaagg agtagccgac
     4021 atatatccga aataactgct tgtttttttt ttttaccatt attaccatcg tgtttactgt
     4081 ttattgcccc ctcaaaaagc taatgtaatt atatttgtgc caataaaaac aagatatgac
     4141 ctatagaata caagtatttc cccttcgaac atccccacaa gtagactttg gatttgtctt
     4201 ctaaccaaaa gacttacaca cctgcatacc ttacatcaaa aactcgttta tcgctacata
     4261 aaacaccggg atatattttt tatatacata cttttcaaat cgcgcgccct cttcataatt
     4321 cacctccacc acaccacgtt tcgtagttgc tctttcgctg tctcccaccc gctctccgca
     4381 acacattcac cttttgttcg acgaccttgg agcgactgtc gttagttccg cgcgattcgg
     4441 ttcgctcaaa tggttccgag tggttcattt cgtctcaata gaaattagta ataaatattt
     4501 gtatgtacaa tttatttgct ccaatatatt tgtatatatt tccctcacag ctatatttat
     4561 tctaatttaa tattatgact ttttaaggta attttttgtg acctgttcgg agtgattagc
     4621 gttacaattt gaactgaaag tgacatccag tgtttgttcc ttgtgtagat gcatctcaaa
     4681 aaaatggtgg gcataatagt gttgtttata tatatcaaaa ataacaacta taataataag
     4741 aatacattta atttagaaaa tgcttggatt tcactggaac taggctagca taacttcgta
     4801 taatgtatgc tatacgaagt tatgctagcg gatccgggaa ttgggaattg ggcaatattt
     4861 aaatggcggc tcgagtccct atcagtgata gagaacgtat atccagttta ctccctatca
     4921 gtgatagaga ttaattaagg agcgccggag tataaataga ggcgcttcgt ctacggagcg
     4981 acaattcaat tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa gcaaataaac
     5041 aagcgcagct gaacaagcta aacaatctgc caggcctccg tagcttaccg aagtatacac
     5101 ttaaattcag tgcacgtttg cttgttgaga ggaaaggttg tgtgcggacg aatttttttt
     5161 tgaaaaccgg tgatagagcc tgaaccagaa aagataaaag aaggctatac cagtgggagt
     5221 acacaaacag agtaagtttg aatagtaaaa aaaatcattt atgtaaacaa taacgtgact
     5281 gtgcgttagg tcctgttcat tggtacccgc ccggggatca gaattgccta ggatggaaga
     5341 cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg
     5401 agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac
     5461 agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt
     5521 ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga
     5581 aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc
     5641 gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc
     5701 taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct
     5761 cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc
     5821 gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtgcc
     5881 agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg gatctactgg
     5941 tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct cgcatgccag
     6001 agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt
     6061 ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt
     6121 cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt acaagattca
     6181 aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc tgattgacaa
     6241 atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct ctaaggaagt
     6301 cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat atgggctcac
     6361 tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg
     6421 taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg
     6481 cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa
     6541 caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat
     6601 agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt ctctgattaa
     6661 gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc aacaccccaa
     6721 catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc
     6781 cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc
     6841 cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc
     6901 gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa
     6961 gaagggcgga aagatcgccg tggagggcag aggaagtctt ctaacatgcg gtgacgtgga
     7021 ggagaatccc ggccctatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     7081 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     7141 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccacca gcaagctgcc
     7201 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     7261 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     7321 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     7381 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     7441 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     7501 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     7561 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     7621 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     7681 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     7741 cgagctgtac aagtaagcga tcgcctagaa tgaatcgttt ttaaaataac aaatcaattg
     7801 ttttataata ttcgtacgat tctttgatta tgtaataaaa tgtgatcatt aggaagatta
     7861 cgaaaaatat aaaaaatatg agttctgtgt gtataacaaa tgctgtaaac gccacaattg
     7921 tgtttgttgc aaataaaccc atgattattt gattaaaatt gttgttttct ttgttcatag
     7981 acaatagtgt gttttgccta aacgtgtact gcataaactc catgcgagtg tatagcgagc
     8041 tagtggctaa cgcttgcccc accaaagtag attcgtcaaa atcctcaatt tcatcaccct
     8101 cctccaagtt taacatttgg ccgtcggaat taacttctaa agatgccaca taatctaata
     8161 aatgaaatag agattcaaac gtggcgtcat cgtccgtttc gaccatttcc gaaaagaact
     8221 cgggcataaa ctctatgatt tctctggacg tggtgttgtc gaaactctca aagtacgcag
     8281 tcaggaacgt gcgcgacatg tcgtcgggaa actcgcgcgg aaacatgttg ttgtaaccga
     8341 acgggtccca tagcgccaaa accaaatctg ccagcgtcaa tagaatgagc acgatgccga
     8401 caatggagct ggcttggata gcgattcgag ttaacggccc ttaagggtcg actgatcata
     8461 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     8521 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     8581 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     8641 cattctagtt gtggtttgtc caaactcatc aatgtggcgc gccttattgt tcatttttga
     8701 gaactcgctc aacgaacgat ttgatatatt ttcccatttc atcaggtgca tcttcttgcg
     8761 aaaaatgaag accttttact ttgacaaatt cagtattagg aaacttcttg gcaccttcaa
     8821 caatagcatt ggaaaagaat cctgggtccg attcaataaa catttttggt aaatcatcac
     8881 ttgcacgtag ataagcatta taattcctaa caatttgtac aacgtcaggt ttaccacctt
     8941 ttactaacgg gatttcacga ggccatgata atgttggacg acgaacttca cctttctctt
     9001 tgaatggttc aagatatgct gcaaattctt ctggttctaa ctttctcatg atttttgatg
     9061 gcaacatggt ttccacgaag aagttattct ccaaaaccat tttttctcct tcttcagatt
     9121 tgatcaacgc aatatcttct tcaatatcag gccattcatc ccatgattca atcacatcta
     9181 ctacactttc agcgtgaact attgctttga tcttatcttg atgctcatag ctataatgaa
     9241 atgccaaaca agcaccccaa tcatggccga caaaaatgat cttctttggt aaattaagaa
     9301 gttcaaacca tgcagtaaga tatttgtaat gatcaagtaa cctataagaa ccattaccag
     9361 atttgcctga tttgcccata ccaataaggt ctggtataat acaccgcgct actggctcaa
     9421 tatgtggcac aacatgtcgc cataaataag aagaggccgc gttaccatgt aaaaaaataa
     9481 cagcattttc tgcatgtttt tctgaatcat aataattaat aaatgaatca agaacattca
     9541 tttgtttaca tctggcccac cactgcggac cagttatcat ccgtttcctt tgttctggat
     9601 cataaacttt cgaagtcatg cggccgctct gcgggtcaaa atagagatgt ggaaaattag
     9661 tacgaaatca aatgagtttc gttgaaatta caaaactatt gaaactaact tcctggctgg
     9721 ggaataaaaa tgggaaactt atttatcgac gccaactttg ttgagaaacc cctattaacc
     9781 ctctacgaat attggaacaa aggaaagcga agaaacaggg aacaaagggt agttgagaaa
     9841 cctttgttcc gttgcttcgt catcgttttc gtgttgcgag tgtgtgcatg tatatataca
     9901 cagctgaaat gcatgcatac acattatttt gtgtgtatat ggtgacgtca caactacaag
     9961 caataagaaa ttttcagacg tggctttgtt taagcaacct actctatttc agctaaaaat
    10021 aagtggattt cgttggtaaa atacttcaat taactaaaca actaactaat aacatgcaca
    10081 caaatgcaat atgtgcgttc gtgatttctc gaattttcaa atgcgtcact gcgaatttca
    10141 caatttgcca ataaatcttg gcgaaaatca acacgcaagt tttatttata gatttgtttg
    10201 cgttttgatg ccaattcatt gggaaaacaa gatgcgtggc tgccaatttc ttattttgta
    10261 attacgtaga gcgttgaata aaaaaaaaat ggccgaacaa agaccttgaa atgcagtttt
    10321 tcttgaaatt actcaacgtc ttgttgctct tattactaat tggtaacagc gagttaaaaa
    10381 cttacgtttc ttgtgacttt cgagaatgtt cttttaattg tactttaatc accaacaatt
    10441 aagtataaat ttttcgctga ttgcgcttta ctttctactt gtacttgctg ctgcaaatgt
    10501 caattggttt tgaaggcgac cgttcgcgaa cgctgtttat ataccttcgg tgtccgttga
    10561 aaatcactaa aaaataccgt agtgttcgta acactttagt acagagaaaa aaaattgtgc
    10621 cgaaatgttt ttgatacgta cgaatacctt gtattaaaat tttttatgat ttctgtgtat
    10681 cacttttttt ttgtgttttt cgtttaaact caccacagta caaaacaata aaatattttt
    10741 aagacaattt caaattgaga cctttctcgt actgacttga ccggctgaat gaggatttct
    10801 acctagacga cctacttctt accatgacat tgaatgcaat gccacctttg atctaaactt
    10861 acaaaagtcc aaggcttgtt aggattggtg ttttggaatt taagaccctt gttagtttgc
    10921 ttttgaaata gcactgtctt ctctaccggc tataattttg aaactcgcag cttgactgga
    10981 aatttaaaaa gtaattctgt gtaggtaaag ggtgttttaa aagtgtgatg tgttgagcgt
    11041 tgcggcaacg actgctattt atgtatatat tttcaaaact tattgttttt gaagtgtttt
    11101 aaatggagct atctggcaac gctgcgcata atcttacaca agcttttctt aatccatttt
    11161 taagtgaaat ttgtttatac tctttcggca aataattgtt aaatcgcttt aagtgggctt
    11221 acatctggat aagtaatgaa aacctgcata ttataatatt aaaacatata atccactgtg
    11281 ctttccccgt gtgtggccat atacctaaaa aagtttattt tcgcagagcc ccgcacggtc
    11341 acactacggt tcggcgattt tcgattttgg acagtactga ttgcaagcgc accgaaagca
    11401 aaatggagct ggagattttg aacgcgaaga acagcaagcc gtacggcaag gtgaaggtgc
    11461 cctccggcgc cacgcccatc ggcgatctgc gcgtctagag atccactagt gtcgacgatg
    11521 taggtcacgg tctcgaagcc gcggtgcggg tgccagggcg tgcccttggg ctccccgggc
    11581 gcgtactcca cctcacccat ctggtccatc atgatgaacg ggtcgaggtg gcggtagttg
    11641 atcccggcga acgcgcggcg caccgggaag ccctcgccct cgaaaccgct gggcgcggtg
    11701 gtcacggtga gcacgggacg tgcgacggcg tcggcgggtg cggatacgcg gggcagcgtc
    11761 agcgggttct cgacggtcac ggcgggcatg tcgacactag ttctagccag cttttgttcc
    11821 ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt tcctgtgtga
    11881 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
    11941 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
    12001 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    12061 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    12121 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    12181 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    12241 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    12301 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    12361 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    12421 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    12481 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    12541 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    12601 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    12661 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
    12721 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    12781 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    12841 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    12901 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    12961 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    13021 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    13081 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    13141 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    13201 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    13261 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    13321 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    13381 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    13441 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    13501 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    13561 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    13621 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    13681 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    13741 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    13801 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    13861 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    13921 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    13981 ccgcgcacat ttccccgaaa agtgccacct
//
