LOCUS       sequence-31602-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NEDD4L-C2
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..87
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             579..596
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(649..667)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      663..710
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(834..853)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 taattttgtt taactttaag aaggagatat accatgcatc atcatcatca tcacagcagc
       61 ggcagagaaa acttgtattt ccagggcatg gcgaccgggc tcggggagcc ggtctatgga
      121 ctttccgaag acgagggaga gtcccgtatt ctcagagtaa aagttgtttc tggaattgat
      181 ctcgccaaaa aggacatctt tggagccagt gatccgtatg tgaaactttc attgtacgta
      241 gcggatgaga atagagaact tgctttggtc cagacaaaaa caattaaaaa gacactgaac
      301 ccaaaatgga atgaagaatt ttatttcagg gtaaacccat ctaatcacag actcctattt
      361 gaagtatttg acgaaaatag actgacacga gacgacttcc tgggccaggt ggacgtgccc
      421 cttagtcacc ttccgacaga agatccaacc atggagcgac cctatacatt taaggacttt
      481 ctcctcagac caagaagtca taagtctcga gttaagggat ttttgcgatt gaaaatggcc
      541 tatatgccat gatgacgaag cttgcggccg cactcgagca ccaccaccac caccactgag
      601 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
      661 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
      721 gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
      781 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
      841 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
      901 ggggctccct tta
//
