LOCUS       sequence-316029-       14006 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  VanO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3'UTR-SV40-Ubiquitin-
            Renilla-attB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14006)
  AUTHORS   Gamez S, Vesga LC, Mendez-Sanchez SC, Akbari OS
  TITLE     Spatial control of gene expression in flies using bacterially 
            derived binary transactivation systems.
  JOURNAL   Insect Mol Biol. 2021 Oct;30(5):461-471. doi: 10.1111/imb.12717. 
            Epub 2021 May 24.
  PUBMED    33963794
REFERENCE   2  (bases 1 to 14006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/imb.12717";
            journalName: "Insect Mol Biol"; date: "2021-10"; volume: "30"; 
            issue: "5"; pages: "461-471"
COMMENT     Sequence Label: 908I
FEATURES             Location/Qualifiers
     source          1..14006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     621..640
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        621..639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(673..691)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1143..1214,1616..1889,1964..2618,2680..2995,3199..3330,
                     3401..4015)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4790..4823
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5329..6978
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(5395..5413)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             6979..7032
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(7033..7035,7036..7038,7039..7752)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTSKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7078..7099)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7339..7358)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7686..7707
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8556..8637
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8593..8612)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8647..8666
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(8680..9615)
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     protein_bind    11528..11597
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(11816..11834)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11855..11871)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11855..11871)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11868..11890)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11879..11895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11903..11909,11910..11927,11928..11933))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11948..11969
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12086..12103)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12257..12845)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12337..12356)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13016..13807,13808..13876))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13639..13658
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13877..13981)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggt acgtaccggg
      661 cccctagtat gtatgtaagt taataaaacc catttttgcg gaaagtagat aaaaaaaaca
      721 tttttttttt ttactgcact ggatatcatt gaacttatct gatcagtttt aaatttactt
      781 cgatccaagg gtatttgatg taccaggttc tttcgattac ctctcactca aaatgacatt
      841 ccactcaaag tcagcgctgt ttgcctcctt ctctgtccac agaaatatcg ccgtctcttt
      901 cgccgctgcg tccgctatct ctttcgccac cgtttgtagc gttacgtagc gtcaatgtcc
      961 gccttcagtt gcattttgtc agcggtttcg tgacgaagct ccaagcggtt tacgccatca
     1021 attaaacaca aagtgctgtg ccaaaactcc tctcgcttct tatttttgtt tgttttttga
     1081 gtgattgggg tggtgattgg ttttgggtgg gtaagcaggg gaaagtgtga aaaatcccgg
     1141 caatgggcca agaggatcag gagctattaa ttcgcggagg cagcaaacac ccatctgccg
     1201 agcatctgaa caatgtgagt agtacatgtg catacatctt aagttcactt gatctatagg
     1261 aactgcgatt gcaacatcaa attgtctgcg gcgtgagaac tgcgacccac aaaaatccca
     1321 aaccgcaatt gcacaaacaa atagtgacac gaaacagatt attctggtag ctgttctcgc
     1381 tatataagac aatttttgag atcatatcat gatcaagaca tctaaaggca ttcattttcg
     1441 actatattct tttttacaaa aaatataaca accagatatt ttaagctgat cctagatgca
     1501 caaaaaataa ataaaagtat aaacctactt cgtaggatac ttcggggtac tttttgttcg
     1561 gggttagatg agcataacgc ttgtagttga tatttgagat cccctatcat tgcagggtga
     1621 cagcggagcg gcttcgcaga gctgcattaa ccagggcttc gggcaggcca aaaactacgg
     1681 cacgctccgg ccacccagtc cgccggagga ctccggttca gggagcggcc aactagccga
     1741 gaacctcacc tatgcctggc acaatatgga catctttggg gcggtcaatc agccgggctc
     1801 cggatggcgg cagctggtca accggacacg cggactattc tgcaacgagc gacacatacc
     1861 ggcgcccagg aaacatttgc tcaagaacgg tgagtttcta ttcgcagtcg gctgatctgt
     1921 gtgaaatctt aataaagggt ccaattacca atttgaaact cagtttgcgg cgtggcctat
     1981 ccgggcgaac ttttggccgt gatgggcagt tccggtgccg gaaagacgac cctgctgaat
     2041 gcccttgcct ttcgatcgcc gcagggcatc caagtatcgc catccgggat gcgactgctc
     2101 aatggccaac ctgtggacgc caaggagatg caggccaggt gcgcctatgt ccagcaggat
     2161 gacctcttta tcggctccct aacggccagg gaacacctga ttttccaagc catggtgcgg
     2221 atgccacgac atctgaccta tcggcagcga gtggcccgcg tggatcaggt gatccaggag
     2281 ctttcgctca gcaaatgtca gcacacgatc atcggtgtgc ccggcagggt gaaaggtctg
     2341 tccggcggag aaaggaagcg tctggcattc gcctccgagg ctctaaccga tccgccgctt
     2401 ctgatctgcg atgagcccac ctccggactg gactccttta ccgcccacag cgtcgtccag
     2461 gtgctgaaga agctgtcgca gaagggcaag accgtcatcc tgaccattca tcagccgtct
     2521 tccgagctgt ttgagctctt tgacaagatc cttctgatgg ccgagggcag ggtagctttc
     2581 ttgggcactc ccagcgaagc cgtcgacttc ttttcctagt gagttcgatg tgtttattaa
     2641 gggtatctag tattacataa catctcaact cctatccagc gtgggtgccc agtgtcctac
     2701 caactacaat ccggcggact tttacgtaca ggtgttggcc gttgtgcccg gacgggagat
     2761 cgagtcccgt gatcggatcg ccaagatatg cgacaatttt gccattagca aagtagcccg
     2821 ggatatggag cagttgttgg ccaccaaaaa tctggagaag ccactggagc agccggagaa
     2881 tgggtacacc tacaaggcca cctggttcat gcagttccgg gcggtcctgt ggcgatcctg
     2941 gctgtcggtg ctcaaggaac cactcctcgt aaaagtgcga cttattcaga caacggtgag
     3001 tggttccagt ggaaacaaat gatataacgc ttacaattct tggaaacaaa ttcgctagat
     3061 tttagataga attgcctgat tccacaccct tcttagtttt tttcaatgag atgtatagtt
     3121 tatagttttg cagaagataa ataaatttca tttaactcgc gaatattaat gagatgcgag
     3181 taacatttta atttgcagat ggttgccatc ttgattggcc tcatcttttt gggccaacaa
     3241 ctcacgcaag tgggtgtgat gaatatcaac ggagccatct tcctcttcct gaccaacatg
     3301 acctttcaaa acgtctttgc cacgataaat gtaagtcatg tttagaatac atttgcattt
     3361 caataattta ctaactttct aatgaatcga ttcgatttag gtgttcacct cagagctgcc
     3421 agtttttatg agggaggccc gaagtcgact ttatcgctgt gacacatact ttctgggcaa
     3481 aacgattgcc gaattgccgc tttttctcac agtgccactg gtcttcacgg cgattgccta
     3541 tccgatgatc ggactgcggg ccggagtgct gcacttcttc aactgcctgg cgctggtcac
     3601 tctggtggcc aatgtgtcaa cgtccttcgg atatctaata tcctgcgcca gctcctcgac
     3661 ctcgatggcg ctgtctgtgg gtccgccggt tatcatacca ttcctgctct ttggcggctt
     3721 cttcttgaac tcgggctcgg tgccagtata cctcaaatgg ttgtcgtacc tctcatggtt
     3781 ccgttacgcc aacgagggtc tgctgattaa ccaatgggcg gacgtggagc cgggcgaaat
     3841 tagctgcaca tcgtcgaaca ccacgtgccc cagttcgggc aaggtcatcc tggagacgct
     3901 taacttctcc gccgccgatc tgccgctgga ctacgtgggt ctggccattc tcatcgtgag
     3961 cttccgggtg ctcgcatatc tggctctaag acttcgggcc cgacgcaagg agtagccgac
     4021 atatatccga aataactgct tgtttttttt ttttaccatt attaccatcg tgtttactgt
     4081 ttattgcccc ctcaaaaagc taatgtaatt atatttgtgc caataaaaac aagatatgac
     4141 ctatagaata caagtatttc cccttcgaac atccccacaa gtagactttg gatttgtctt
     4201 ctaaccaaaa gacttacaca cctgcatacc ttacatcaaa aactcgttta tcgctacata
     4261 aaacaccggg atatattttt tatatacata cttttcaaat cgcgcgccct cttcataatt
     4321 cacctccacc acaccacgtt tcgtagttgc tctttcgctg tctcccaccc gctctccgca
     4381 acacattcac cttttgttcg acgaccttgg agcgactgtc gttagttccg cgcgattcgg
     4441 ttcgctcaaa tggttccgag tggttcattt cgtctcaata gaaattagta ataaatattt
     4501 gtatgtacaa tttatttgct ccaatatatt tgtatatatt tccctcacag ctatatttat
     4561 tctaatttaa tattatgact ttttaaggta attttttgtg acctgttcgg agtgattagc
     4621 gttacaattt gaactgaaag tgacatccag tgtttgttcc ttgtgtagat gcatctcaaa
     4681 aaaatggtgg gcataatagt gttgtttata tatatcaaaa ataacaacta taataataag
     4741 aatacattta atttagaaaa tgcttggatt tcactggaac taggctagca taacttcgta
     4801 taatgtatgc tatacgaagt tatgctagcg gatccgggaa ttgggaattg ggcaatattt
     4861 aaatggcggc tcgagattgg atccaatctt gacgtccgtt tgattacgat caagattgga
     4921 tccaatttaa ttaaggagcg ccggagtata aatagaggcg cttcgtctac ggagcgacaa
     4981 ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc
     5041 gcagctgaac aagctaaaca atctgccagg cctccgtagc ttaccgaagt atacacttaa
     5101 attcagtgca cgtttgcttg ttgagaggaa aggttgtgtg cggacgaatt tttttttgaa
     5161 aaccggtgat agagcctgaa ccagaaaaga taaaagaagg ctataccagt gggagtacac
     5221 aaacagagta agtttgaata gtaaaaaaaa tcatttatgt aaacaataac gtgactgtgc
     5281 gttaggtcct gttcattggt acccgcccgg ggatcagaat tgcctaggat ggaagacgcc
     5341 aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag
     5401 caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat
     5461 gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca
     5521 gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac
     5581 tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc
     5641 gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc
     5701 gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca
     5761 atcatccaaa aaattattat catggattct aaaacggatt accagggatt tcagtcgatg
     5821 tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag
     5881 tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg
     5941 cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat
     6001 cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat
     6061 cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta
     6121 atgtatagat ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt
     6181 gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac
     6241 gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg
     6301 gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag
     6361 actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa
     6421 gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt
     6481 aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat
     6541 ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct
     6601 tactgggacg aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac
     6661 aaaggctatc aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc
     6721 ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt
     6781 gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt
     6841 caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa
     6901 ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag
     6961 ggcggaaaga tcgccgtgga gggcagagga agtcttctaa catgcggtga cgtggaggag
     7021 aatcccggcc ctatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     7081 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     7141 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccagcaa gctgcccgtg
     7201 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     7261 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     7321 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     7381 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     7441 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     7501 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     7561 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     7621 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     7681 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     7741 ctgtacaagt aagcgatcgc ctagaatgaa tcgtttttaa aataacaaat caattgtttt
     7801 ataatattcg tacgattctt tgattatgta ataaaatgtg atcattagga agattacgaa
     7861 aaatataaaa aatatgagtt ctgtgtgtat aacaaatgct gtaaacgcca caattgtgtt
     7921 tgttgcaaat aaacccatga ttatttgatt aaaattgttg ttttctttgt tcatagacaa
     7981 tagtgtgttt tgcctaaacg tgtactgcat aaactccatg cgagtgtata gcgagctagt
     8041 ggctaacgct tgccccacca aagtagattc gtcaaaatcc tcaatttcat caccctcctc
     8101 caagtttaac atttggccgt cggaattaac ttctaaagat gccacataat ctaataaatg
     8161 aaatagagat tcaaacgtgg cgtcatcgtc cgtttcgacc atttccgaaa agaactcggg
     8221 cataaactct atgatttctc tggacgtggt gttgtcgaaa ctctcaaagt acgcagtcag
     8281 gaacgtgcgc gacatgtcgt cgggaaactc gcgcggaaac atgttgttgt aaccgaacgg
     8341 gtcccatagc gccaaaacca aatctgccag cgtcaataga atgagcacga tgccgacaat
     8401 ggagctggct tggatagcga ttcgagttaa cggcccttaa gggtcgactg atcataatca
     8461 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     8521 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     8581 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     8641 ctagttgtgg tttgtccaaa ctcatcaatg tggcgcgcct tattgttcat ttttgagaac
     8701 tcgctcaacg aacgatttga tatattttcc catttcatca ggtgcatctt cttgcgaaaa
     8761 atgaagacct tttactttga caaattcagt attaggaaac ttcttggcac cttcaacaat
     8821 agcattggaa aagaatcctg ggtccgattc aataaacatt tttggtaaat catcacttgc
     8881 acgtagataa gcattataat tcctaacaat ttgtacaacg tcaggtttac caccttttac
     8941 taacgggatt tcacgaggcc atgataatgt tggacgacga acttcacctt tctctttgaa
     9001 tggttcaaga tatgctgcaa attcttctgg ttctaacttt ctcatgattt ttgatggcaa
     9061 catggtttcc acgaagaagt tattctccaa aaccattttt tctccttctt cagatttgat
     9121 caacgcaata tcttcttcaa tatcaggcca ttcatcccat gattcaatca catctactac
     9181 actttcagcg tgaactattg ctttgatctt atcttgatgc tcatagctat aatgaaatgc
     9241 caaacaagca ccccaatcat ggccgacaaa aatgatcttc tttggtaaat taagaagttc
     9301 aaaccatgca gtaagatatt tgtaatgatc aagtaaccta taagaaccat taccagattt
     9361 gcctgatttg cccataccaa taaggtctgg tataatacac cgcgctactg gctcaatatg
     9421 tggcacaaca tgtcgccata aataagaaga ggccgcgtta ccatgtaaaa aaataacagc
     9481 attttctgca tgtttttctg aatcataata attaataaat gaatcaagaa cattcatttg
     9541 tttacatctg gcccaccact gcggaccagt tatcatccgt ttcctttgtt ctggatcata
     9601 aactttcgaa gtcatgcggc cgctctgcgg gtcaaaatag agatgtggaa aattagtacg
     9661 aaatcaaatg agtttcgttg aaattacaaa actattgaaa ctaacttcct ggctggggaa
     9721 taaaaatggg aaacttattt atcgacgcca actttgttga gaaaccccta ttaaccctct
     9781 acgaatattg gaacaaagga aagcgaagaa acagggaaca aagggtagtt gagaaacctt
     9841 tgttccgttg cttcgtcatc gttttcgtgt tgcgagtgtg tgcatgtata tatacacagc
     9901 tgaaatgcat gcatacacat tattttgtgt gtatatggtg acgtcacaac tacaagcaat
     9961 aagaaatttt cagacgtggc tttgtttaag caacctactc tatttcagct aaaaataagt
    10021 ggatttcgtt ggtaaaatac ttcaattaac taaacaacta actaataaca tgcacacaaa
    10081 tgcaatatgt gcgttcgtga tttctcgaat tttcaaatgc gtcactgcga atttcacaat
    10141 ttgccaataa atcttggcga aaatcaacac gcaagtttta tttatagatt tgtttgcgtt
    10201 ttgatgccaa ttcattggga aaacaagatg cgtggctgcc aatttcttat tttgtaatta
    10261 cgtagagcgt tgaataaaaa aaaaatggcc gaacaaagac cttgaaatgc agtttttctt
    10321 gaaattactc aacgtcttgt tgctcttatt actaattggt aacagcgagt taaaaactta
    10381 cgtttcttgt gactttcgag aatgttcttt taattgtact ttaatcacca acaattaagt
    10441 ataaattttt cgctgattgc gctttacttt ctacttgtac ttgctgctgc aaatgtcaat
    10501 tggttttgaa ggcgaccgtt cgcgaacgct gtttatatac cttcggtgtc cgttgaaaat
    10561 cactaaaaaa taccgtagtg ttcgtaacac tttagtacag agaaaaaaaa ttgtgccgaa
    10621 atgtttttga tacgtacgaa taccttgtat taaaattttt tatgatttct gtgtatcact
    10681 ttttttttgt gtttttcgtt taaactcacc acagtacaaa acaataaaat atttttaaga
    10741 caatttcaaa ttgagacctt tctcgtactg acttgaccgg ctgaatgagg atttctacct
    10801 agacgaccta cttcttacca tgacattgaa tgcaatgcca cctttgatct aaacttacaa
    10861 aagtccaagg cttgttagga ttggtgtttt ggaatttaag acccttgtta gtttgctttt
    10921 gaaatagcac tgtcttctct accggctata attttgaaac tcgcagcttg actggaaatt
    10981 taaaaagtaa ttctgtgtag gtaaagggtg ttttaaaagt gtgatgtgtt gagcgttgcg
    11041 gcaacgactg ctatttatgt atatattttc aaaacttatt gtttttgaag tgttttaaat
    11101 ggagctatct ggcaacgctg cgcataatct tacacaagct tttcttaatc catttttaag
    11161 tgaaatttgt ttatactctt tcggcaaata attgttaaat cgctttaagt gggcttacat
    11221 ctggataagt aatgaaaacc tgcatattat aatattaaaa catataatcc actgtgcttt
    11281 ccccgtgtgt ggccatatac ctaaaaaagt ttattttcgc agagccccgc acggtcacac
    11341 tacggttcgg cgattttcga ttttggacag tactgattgc aagcgcaccg aaagcaaaat
    11401 ggagctggag attttgaacg cgaagaacag caagccgtac ggcaaggtga aggtgccctc
    11461 cggcgccacg cccatcggcg atctgcgcgt ctagagatcc actagtgtcg acgatgtagg
    11521 tcacggtctc gaagccgcgg tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt
    11581 actccacctc acccatctgg tccatcatga tgaacgggtc gaggtggcgg tagttgatcc
    11641 cggcgaacgc gcggcgcacc gggaagccct cgccctcgaa accgctgggc gcggtggtca
    11701 cggtgagcac gggacgtgcg acggcgtcgg cgggtgcgga tacgcggggc agcgtcagcg
    11761 ggttctcgac ggtcacggcg ggcatgtcga cactagttct agccagcttt tgttcccttt
    11821 agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
    11881 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
    11941 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
    12001 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    12061 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    12121 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    12181 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    12241 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    12301 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    12361 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    12421 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    12481 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    12541 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    12601 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    12661 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    12721 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    12781 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    12841 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    12901 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    12961 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    13021 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    13081 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    13141 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    13201 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    13261 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    13321 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    13381 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    13441 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    13501 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    13561 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    13621 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    13681 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    13741 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    13801 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    13861 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    13921 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    13981 gcacatttcc ccgaaaagtg ccacct
//
