LOCUS       sequence-31603-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PRKCH
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             10..32
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..95
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             539..556
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(609..627)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      623..670
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(794..813)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tagaataatt ttgtttaact ttaagaagga gatataccat gggcagcagc catcatcatc
       61 atcatcacag cagcggcctg gttccgcgtg gtagtatgtc gtctggcacc atgaagttca
      121 atggctattt gagggtccgc atcggtgagg cagtggggct gcagcccacc cgctggtccc
      181 tgcgccactc gctcttcaag aagggccacc agctgctgga cccctatctg acggtgagcg
      241 tggaccaggt gcgcgtgggc cagaccagca ccaagcagaa gaccaacaaa cccacgtaca
      301 acgaggagtt ttgcgctaac gtcaccgacg gcggccacct cgagttggcc gtcttccacg
      361 agacgcccct gggctacgac cacttcgtgg ccaactgcac cctgcagttc caggagctgc
      421 tgcgcacgac cggcgcctcg gacaccttcg agggttgggt ggatctcgag ccagagggga
      481 aagtattcgt ggtaataacc cttaccgggt gatgacgaag cttgcggccg cactcgagca
      541 ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc
      601 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
      661 gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc
      721 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
      781 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
      841 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
      901 ctcgacccca aaaacttgat ta
//
