LOCUS       sequence-316039-       12617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses pHnCB10 derived carboxysome operon and prk.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12617)
  AUTHORS   Flamholz AI, Dugan E, Blikstad C, Gleizer S, Ben-Nissan R, Amram S, 
            Antonovsky N, Ravishankar S, Noor E, Bar-Even A, Milo R, Savage D
  TITLE     Functional reconstitution of a bacterial CO2 concentrating mechanism
            in E. coli.
  JOURNAL   Elife. 2020 Oct 21;9. pii: 59882. doi: 10.7554/eLife.59882.
  PUBMED    33084575
REFERENCE   2  (bases 1 to 12617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-10-21"; volume: "9. pii: 59882. doi"; pages: " 
            10.7554/eLife.59882"
COMMENT     Sequence Label: pCB
FEATURES             Location/Qualifiers
     source          1..12617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5531..5586
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    5537..5555
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    5567..5585
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             5632..5649
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      6699..6785
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(6958..7581)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     7489..7508
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        complement(7582..7686)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7862..8450)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7942..7961)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(8538..8632)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(8663..9457)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8774..8793)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9384..9403
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9636..9654
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(9675..9693,9694..9699,9700..9716,9717..9722,
                     9723..9748)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    9675..9693
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9700..9718
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             9758..9769
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 cctgttagcg gtacggaagt ttcacggacc gagaaggtaa ctggcaacga atccggtgtc
       61 tgccgtaacg ttaccggcac cgaatacatg agtaatgaag ctcacttttc tctatgtgga
      121 acagccgcaa agccttcaca agcggataaa gtcatgttcg gcgccacagc acgaacgcat
      181 caagtggtca gtggcagtga tgaattcagg ccctcttctg ttacgggtaa cgaatcgggt
      241 gcaaaacgca caattaccgg ctcgcagtac gcagacgaag gtcttgcgcg actcacgatc
      301 aacggagcac ctgcaaaagt agccagaacc cacacctttg cgggctctga cgttaccggc
      361 acggaaatcg gtcgctctac tcgcgtaact ggtgatgaaa gcggttcgtg tcgttcaatc
      421 tcaggcaccg agtatctcag taacgagcaa ttccaatcct tttgtgacac aaaacctcaa
      481 cgcagcccgt tcaaggttgg ccaagatcgc acgaacaagg gtcagtctgt gactggtaac
      541 ttggttgatc gttccgaact ggttacaggt aacgaaccag gttcatgctc tcgggttaca
      601 ggctctcagt atggccaaag caaaatctgc ggtggtggcg tgggaaaagt gcgctcaatg
      661 cgcacccttc gcggcacctc agtatctggc caacagctag atcatgcccc aaagatgtcc
      721 ggtgacgagc gcggcgggtg catgcccgtc accggtaatg agtactacgg tcgtgaacat
      781 ttcgaaccgt tttgtacgag caccccagag cccgaagctc aatcaactga acaatcattg
      841 acctgtgaag gacaaattat tagcggcact tcagttgacg ccagtgattt ggtcacagga
      901 aatgaaatcg gtgaacagca actcatcagc ggtgacgcct atgttggcgc gcagcagaca
      961 ggttgccttc ccactagtcc acgcttcaac caaactggca atgttcagtc aatgggtttt
     1021 aagaacacca atcagccaga acaaaacttt gcaccaggtg aagtaatgcc tactgacttt
     1081 agtattcaaa ccccagctcg ctcggctcag aatcgcatta caggtaacga cattgcgccc
     1141 tcaggtcgca ttacaggccc tggtatgctg gcaaccggct tgattacagg aacccccgaa
     1201 ttcaggcacg ctgcgcgcga gttggttggt tctccacaac ccatggcaat ggccatggcc
     1261 aaccgtaata aggctgctca agcacctgtt gtgcagccag aagtggttgc aactcaggaa
     1321 aagcctgagt tggtatgtgc accaagaagc gatcaaatgg atcgtgtgag tggcgaaggc
     1381 aaagaacgtt gccacatcac tggcgatgac tggtcagtta acaagcacat caccggtaca
     1441 gccggtcaat gggcgagtgg tcgcaaccct tccatgcgcg gtaatgcgcg tgtggtcgaa
     1501 accagcgcgt ttgccaatcg caatgtgcca aaacctgaaa agccgggctc caagatcacg
     1561 ggcagtagtg gtaatgacac ccaaggtagt ctgatcactt actccggcgg cgcgcgcggt
     1621 tgattaagta aagtgtaacg atcatgaaca cccgtaacac acgaagcaag caacgcgcac
     1681 cgtttggtgt tagctcatca gtcaaacctc ggcttgactt gattgagcaa gcaccaaacc
     1741 ctgcctatga ccgccatcct gcttgcataa cgttgcctga gcgtacctgc cggcacccgt
     1801 taactgacct tgaagccaac gaacaactgg gtcgttgcga ggatagcgtc aagaaccgtt
     1861 ttgatcgcgt tatccctttc ttgcaagttg ttgctggcat tcctcttggt ctggattatg
     1921 ttacccgtgt tcaagaatta gcccagtcgt cgcttggaca tacgctgccc gaagaattac
     1981 tcaaagataa ttggatcagt gggcacaatt taaaaggcat ttttggctac gcgactgcta
     2041 aagcactaac agcagccacg gaacaattca gtcgtaaaat aatgtctgag aaggacgatt
     2101 ccgcatcggc cattggcttc ttcctggatt gcgggttcca cgcagtggac ataagccctt
     2161 gtgccgatgg tcgtctcaaa gggttactgc cttacatatt gcgtttgccc cttacggcat
     2221 tcacctatcg taaagcctac gcaggctcga tgttcgatat tgaagatgat ctggcacagt
     2281 gggagaaaaa tgaactccgc cgttatcgtg aaggtgttcc aaatacagcg gatcagccaa
     2341 cacgatacct gaaaattgct gtgtatcatt tcagcacctc tgacccgaca cactctggct
     2401 gcgcggcaca cggcagtaat gatcgtgcag cactggaagc ggctttaacc cagctgatga
     2461 aattcagaga agcggttgaa aatgcccatt gctgcggcgc aagtatcgat attttactga
     2521 ttggcgttga tacggatacg gatgccattc gcgttcatat tccggatagc aaaggttttt
     2581 tgaatccgta tcgttatgtt gacaacacag taacttatgc gcaaacacta catctggcgc
     2641 cggatgaggc tcgtgtgatt attcacgaag caattctcaa cgcaaaccgc agcgatggtt
     2701 gggctaaagg aaatggagta gccagcgagg ggatgcgtcg ttttattggt cagcttttga
     2761 tcaacaacct ctcgcaaatc gattacgtag taaatcgtca tggtggtcgc tatccaccca
     2821 atgatattgg tcatgctgag cgatatatca gtgttggtga tggttttgat gaagttcaaa
     2881 tccggaattt agcctactac gcgcatttgg atacggttga agaaaatgcg attgatgtgg
     2941 atgtgggaat caaaattttc accaaactta atttgagtcg aggtttaccg attccgattg
     3001 ccatccacta tcgctatgac cccaatgttc caggctccag agaaagaacc gtggtcaaag
     3061 caagacggat atacaacgcc attaaagagc ggttctcatc cttggatgag cagaatctat
     3121 tgcagtttcg tttgagcgtt caggcgcagg atatcggaag cccgattgaa gaggttgcat
     3181 ccgcatgaaa atcatgcaag ttgagaaaac gttggtttca acaaaccgta ttgctgatat
     3241 gggtcacaaa ccactattag tggtatggga gaaaccgggc gcgcccaggc aggtcgccgt
     3301 ggatgcgatt ggctgcatac cgggcgattg ggttttgtgc gttgggtcat cggcagcacg
     3361 agaggctgca ggaagcaagt cttatccctc tgatttgacg attatcggga ttattgatca
     3421 gtggaatggt gagtaatgga agtaatgcgc gttcgttccg acctaatcgc aacacgcagg
     3481 attcccggtc ttaaaaatat ctctttgcgt gtcatggagg atgctacggg taaggtcagt
     3541 gtcgcttgcg atcccattgg cgttcccgag ggatgttggg tctttacgat tagcggctct
     3601 gccgctcggt ttggcgtggg tgattttgag attctcacgg atttgacgat tggtggcatc
     3661 atcgatcact gggtaacttg accatcgcta gatgagttga ttttgaatga gtctttattg
     3721 aggagagaag aaatggcagc agtaacaggt attgcactgg gtatgattga aacacgtggt
     3781 ctggttccag cgattgaagc tgccgatgcc atgaccaagg ccgccgaagt acgtttggtt
     3841 ggccgtcaat ttgttggtgg tggttacgtg accgttttgg tccgtggtga aaccggtgcc
     3901 gtcaacgcag cagttcgtgc gggcgctgat gcctgcgaac gagtcggcga tggtctggtc
     3961 gcggcgcata tcattgcccg tgtccattcc gaagtcgaaa acatcctgcc gaaagcccct
     4021 gaagcttaag gattgggaaa gacgaaccgg cgcaggcttg ttccggttct ttgcataaag
     4081 tgacagctta ggagtttatt taaatggctg atgtaactgg tattgctctg ggtatgatcg
     4141 aaacacgtgg cttagttcct gcgattgaag cagcggacgc catgactaaa gcggctgaag
     4201 tgcgtttggt cggtcgtcaa tttgttggtg gcggttacgt caccgtattg gttcggggcg
     4261 aaacaggcgc tgttaacgcc gctgttcgtg ctggcgccga tgcttgcgaa cgtgttggtg
     4321 atggcttggt tgctgcgcac atcattgcgc gtgtccactc agaagtagaa aacattctgc
     4381 ctaaggcgcc acaagcctaa gtcagatatt cctaagacgg ctcacttcgg cgacacccgt
     4441 cgccgaaacc gtctaaccaa atttctgggc tcgttctgaa cgtgctcaac actagtttta
     4501 gaggatctgt tatggcaacg actcacggta ttgccctggg catgattgaa acacgaggat
     4561 tggttcctgc cattgaagcc gcagatgcca tgaccaaagc ggcggaagtc cgtctggtcg
     4621 gacgatcatt tgttggcggc ggttacgtga ccgtaatggt tcgtggtgag acaggtgcag
     4681 taaatgctgc cgttcgtgcg ggtgctgacg cctgtgaacg tgttggcgat ggcctggttg
     4741 ctgcgcacat cattgcgcgc gttcattctg aagttgagat catcctaccc gagacgcccg
     4801 aagactcaga ttccgcgtgg tgtatcgcaa atctgaatag ctaatgtcta gtagggaaga
     4861 tgcgcatgaa caacattgat ttgcgcgtct attcgtttat cgattcgctt caaccgcagc
     4921 ttgcatccta tcttgcgaca tcatcgcaag gctttcttcc cgttccgggt gatgcctgct
     4981 tgtggattga agtcgcgccg ggcatggccg tccatcgcct cagtgatatt gcgctaaagg
     5041 ccacgaacgt tcgtctcggc gaacaggtag tcgagcgtgc ttttggctcg atggaaattc
     5101 attaccgaaa ccaaagcgac gttctcgcat ccggtgaggc cgttttaaga gaaatcaatc
     5161 acgcgcaaga agatcgtctg ccttgtcgca tcgcatggaa agagatcatt cgagcgatta
     5221 cccccgatca tgccaccttg atcaatcgcc agttgcgtaa aggctctatg ctgttgccgg
     5281 gcaaaagcat gttcatcctt gaaacagaac cggcaggtta tattgttcag gctgccaacg
     5341 aggccgagaa agcagctcat gtgactctga tcgatgtacg tgcttttggt aactttggtc
     5401 gcctgaccat gatgggcagc gaagcggaaa ccgaagaagc catgcgggcg gctgaggcca
     5461 caatcgcaag catcaacgcg cgtgcgcgtc gcgctgaagg gttctaaatc atgattaatt
     5521 cctaattttt gttgacactc tatcattgat agagttattt taccactccc tatcagtgat
     5581 agagaaaagt gaaaaaaagc cagccagtag taagaggggg aattccgcat gcatcatcac
     5641 catcaccaca gcaagccaga tcgtgttgtt ttgatcggcg ttgccggtga ctccggttgc
     5701 ggcaaatcaa ccttcctaaa tcgccttgcc gacttgtttg gtacggaatt gatgacggtc
     5761 atctgcttgg atgactatca cagtctcgat cgcaagggcc ggaaggaagc aggcgtaacg
     5821 gctttggatc cccgcgccaa caactttgac ttgatgtatg aacaggtcaa ggcgttgaag
     5881 aacggcgaaa cgatcatgaa gccgatctac aaccatgaaa ccggcttgat cgatccgccc
     5941 gaaaaaatcg aacccaatcg catcattgtg atcgagggtc tgcatccgct ttacgacgag
     6001 cgcgtgcgtg aactgctcga tttcagcgtt tacctcgaca tcgatgacga agtcaaaatc
     6061 gcttggaaga tccaacgcga tatggcagaa cgcggccact cctacgaaga tgtcctcgcc
     6121 tcgatcgaag cgcgccgccc tgacttcaag gcctacattg agccccagcg tggccatgcg
     6181 gacatcgtca tccgcgtcat gccgacccag ctaatcccca atgacaccga gcgcaaggtg
     6241 ctgcgggtgc agttgatcca acgggaaggc cgcgatggtt ttgagccggc ttacctgttc
     6301 gacgaaggtt cgaccatcca gtggacgccc tgcggtcgta agctgacctg ctcctatccg
     6361 ggcattcgct tagcctacgg ccctgacacc tactacggtc acgaagtctc agtgcttgag
     6421 gtcgacggtc agttcgagaa cctcgaagag atgatctacg tcgagggcca cctcagcaag
     6481 accgacacgc agtactacgg tgagttgacc cacctgctgc tacagcacaa agattacccg
     6541 ggttcgaaca acggcacggg tctgttccaa gtgctgaccg gcctgaaaat gcgggcggcc
     6601 tatgagcgtt tgacctccca agcagcaccc gtcgccgcta gtgtctagat ggaattcctg
     6661 cagcccgggg gatcccatgg tacgcgtgct agaggcatca aataaaacga aaggctcagt
     6721 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
     6781 caaatccgcc gccgctagca gttcgtagac ggaaacaaac gcagaatcca agcaaaaggg
     6841 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     6901 catttatcag ggttatgtcc actgggttcg tgccttcatc cggatatcca ggaactgtta
     6961 agacccactt tcacatttaa gttgtttttc taatccgcat atgatcaatt caaggccgaa
     7021 taagaaggct ggctctgcac cttggtgatc aaataattcg atagcttgtc gtaataatgg
     7081 cggcatacta tcagtagtag gtgtttccct ttcttcttta gcgacttgat gctcttgatc
     7141 ttccaatacg caacctaaag taaaatgccc cacagcgctg agtgcatata atgcattctc
     7201 tagtgaaaaa ccttgttggc ataaaaaggc taattgattt tcgagagttt catactgttt
     7261 ttctgtaggc cgtgtaccta aatgtacttt tgctccatcg cgatgactta gtaaagcaca
     7321 tctaaaactt ttagcgttat tacgtaaaaa atcttgccag ctttcccctt ctaaagggca
     7381 aaagtgagta tggtgcctat ctaacatctc aatggctaag gcgtcgagca aagcccgctt
     7441 attttttaca tgccaataca atgtaggctg ctctacacct agcttctggg cgagtttacg
     7501 ggttgttaaa ccttcgattc cgacctcatt aagcagctct aatgcgctgt taatcacttt
     7561 acttttatct aatctagaca tactcttcct ttttcaatat tattgaagca tttatcaggg
     7621 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     7681 tccgcgtttc cctcgacaat tcgcgctaga cctagggcgt tcggctgcgg cgagcggtat
     7741 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7801 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7861 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7921 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7981 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8041 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8101 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8161 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8221 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8281 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     8341 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8401 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8461 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8521 tcatgactag tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg
     8581 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag
     8641 ctctcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc
     8701 gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc
     8761 caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac
     8821 ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca
     8881 agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgcgc gccttgagcc
     8941 tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga
     9001 caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga
     9061 atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata
     9121 ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata
     9181 gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg
     9241 tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca
     9301 ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat
     9361 cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg
     9421 ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct
     9481 cttgatcaga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt
     9541 ttactttgca gggcttccca accttaccag agggcgcccc agctggcaat tccgacgtct
     9601 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     9661 gtcttcacct cgagtcccta tcagtgatag agattgacat ccctatcagt gatagagata
     9721 ctgagcacat cagcaggacg cactgaccga attcattaaa gaggagaaag gtaccatggc
     9781 agttaaaaag tatagtgctg gtgtaaaaga ataccggcag acctattgga tgccggaata
     9841 cacaccgttg gattccgaca tccttgcatg cttcaaaatc accccacaac cgggtgttga
     9901 tcgcgaagaa gccgcagccg cggttgcagc agaatcttca accggcacat ggaccaccgt
     9961 gtggaccgat ttgctgaccg acatggacta ctacaaaggc cgtgcctatc gcattgaaga
    10021 cgtacccggt gacgatgcgg cattctatgc ctttatcgcc tacccaatcg acctgttcga
    10081 agaagggtca gttgttaacg tgtttacctc actggttggt aacgtgttcg gcttcaaagc
    10141 ggtacgcggc ctgcgtctgg aagatgttcg cttcccactc gcctacgtta aaacctgtgg
    10201 cggcccaccg cacggtattc aagtcgaacg cgacaagatg aacaaatatg gtcgcccact
    10261 gttgggttgc accatcaagc caaaacttgg tttgtctgcg aaaaactacg gccgtgccgt
    10321 atacgagtgc ctccgtggcg gcctcgactt cactaaagat gatgaaaaca tcaattctca
    10381 gccgttcatg cgctggcgcg atcgcttctt gttcgtacaa gacgcgaccg aaactgctga
    10441 agcccaaacc ggcgaacgca aaggccatta cctcaacgta acggcgccaa ctcctgaaga
    10501 aatgtacaag cgcgcagaat tcgccaaaga aattggcgcg ccaatcatta tgcacgacta
    10561 catcaccggt ggcttcacgg ccaacactgg cttggccaag tggtgtcaag acaacggcgt
    10621 actgctgcac atccaccgtg cgatgcatgc ggttatcgac cgtaacccga accacggtat
    10681 tcacttccgt gttctgacca agattctgcg tttgtcgggt ggcgatcacc tgcacaccgg
    10741 taccgttgtc ggcaaactgg aaggcgaccg tgcctctact ctgggctgga ttgatttgct
    10801 ccgcgaatcg tttatccctg aagatcgctc gcgcggtatc ttcttcgatc aagactgggg
    10861 ttcaatgcca ggcgtattcg ctgtggcctc tggtggtatt cacgtatggc acatgcctgc
    10921 gctggttaac atctttggtg acgactctgt cctccaattc ggtggcggta cgctgggtca
    10981 tccatggggc aacgctgccg gtgctgctgc caaccgtgtt gctctggaag cctgcgtaga
    11041 agcgcgtaac caaggccgcg atatcgaaaa agaaggcaaa gaaattctga ctgctgctgc
    11101 acagcacagc ccagaactga agattgccat ggaaacttgg aaagagatca aattcgaatt
    11161 tgacactgtc gacaaactcg acactcaaaa tcgttgatcc ctcgtaccac acaacatact
    11221 aaggtgagta accatggctg aaatgcagga ttacaagcaa agcctcaaat atgagacttt
    11281 ctcttatctt ccacccatga acgcggaacg catccgcgct caaatcaagt acgcaattgc
    11341 tcaaggctgg agccccggca ttgagcacgt agaagtgaaa aactccatga accaatattg
    11401 gtacatgtgg aaacttccct tcttcggcga acaaaatgtc gacaacgtgt tggctgaaat
    11461 tgaagcgtgt cgtagtgcgt atccaacaca ccaggtcaaa ctggtggctt atgacaacta
    11521 tgcgcaaagc ttaggtctgg ccttcgtggt ctaccgcggc aactaagtca agtctgtcat
    11581 tgcgctgtgc gtttctctac gcacagcact tattcaagat ggggtcaaac atgccttcac
    11641 agtcaggaat gaatcctgcc gacctgagcg gactctctgg caaggaactg gcacgcgcac
    11701 gccgcgctgc actatccaag caagggaaag cagctgtttc taataaaacg gctagcgtaa
    11761 accgtagcac taaacaggcg gcatcttcga tcaatacaaa tcaggtgcgc tcttctgtaa
    11821 atgaagtgcc cactgattac caaatggcgg atcaattgtg ctctacgatt gatcatgctg
    11881 actttggtac cgaaagcaat cgcgttagag atctctgccg tcaacgcaga gaggcactat
    11941 caactatcgg taaaaaagcg gttaaaacca acggcaagcc gtcgggtcgc gttcgaccac
    12001 agcaatcagt ggttcacaac gacgcaatga tcgaaaatgc cggtgatact aaccaatcat
    12061 cgtccacttc attaaataat gaactttccg aaatctgctc catagcagac gacatgccgg
    12121 agcgttttgg ttcacaagcc aaaaccgtcc gtgatatctg ccgtgcacgc cgtcaagcgc
    12181 tctctgagcg tggaactcgc gccgtgccgc caaagccgca atctcaaggt ggtccaggac
    12241 gcaatggcta tcaaattgat ggatacctag ataccgcact tcatggccgc gatgccgcca
    12301 agcgccaccg tgaaatgctc tgtcaatacg gccgcggcac agcaccttcc tgcaagccaa
    12361 caggccgtgt caaaaattct gtacagtcgg gcaacgcagc gccaaaaaag gttgaaaccg
    12421 gtcacaccct atccggcgga tctgttacgg gcacgcaagt ggatcgtaaa tctcatgtga
    12481 ctggaaacga gccgggcact tgccgagcag tcacgggcac cgagtacgta ggtactgagc
    12541 aattcacctc tttttgcaat accagcccca agccaaatgc gacgaaggtc aatgtgacca
    12601 caacggctcg tggtcgc
//
