LOCUS       sequence-316049-        7878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses in Candida albicans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   Van Genechten W, Demuyser L, Dedecker P, Van Dijck P
  TITLE     Presenting a codon-optimized palette of fluorescent proteins for use
            in Candida albicans.
  JOURNAL   Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.1038/s41598-020-63308-w.
  PUBMED    32273559
REFERENCE   2  (bases 1 to 7878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-04-9"; volume: "10(1):6158. doi"; pages: " 
            10.1038/s41598-020-63308-w"
COMMENT     Sequence Label: CIp10 - YemVenus (OPT High)
FEATURES             Location/Qualifiers
     source          1..7878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..26)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11..27)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(436..454)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(475..491)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(475..491)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(488..510)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    499..515
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(523..529,530..547,548..553))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    568..589
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(706..723)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(878..1467)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(958..977)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1638..2429,2430..2498))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2261..2280
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2499..2603)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2630..3085
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2717..2736)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2927..2948
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3211..3233
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3225..3242
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3226..3242
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3249..3268
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3249..3267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgacgtcatc gataccgtcg acctcgagga gtgaaattct ggaaatctgg aaatctggtt
       61 ttgtattctt gttattcttc tttttgttat tacatatata acttgttact tttttaaaaa
      121 aatctttgtt tattttataa atatataaaa ctaaatttaa gaaaaagaga aaaatgtttt
      181 atttgagaga ttgaaatttt acttgaattt agcttagctt ttataaagta ttattatgta
      241 aaaaaacaaa acaaatatac attaaaaagt taagactata aaatagccac ccaaggcatt
      301 tctatatctt gttgttgttg ttttcatctt ctgtatcaga ggaacttatt ttattatttt
      361 cgtcacgggt attttctctt gtttgatgat tcatcccatt cattccatca aaaatgtcgg
      421 taccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata
      481 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      541 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      601 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
      661 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
      721 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
      781 gttatccaca gaaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
      841 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      901 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      961 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     1021 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     1081 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1141 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1201 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1261 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     1321 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     1381 cttgatccgg caaacaaacc accgctgggt agcggtggtt tttttgtttg caagcagcag
     1441 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1501 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1561 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1621 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     1681 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     1741 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     1801 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     1861 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     1921 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     1981 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2041 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2101 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2161 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2221 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     2281 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     2341 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     2401 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     2461 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     2521 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2581 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt
     2641 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     2701 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     2761 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     2821 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     2881 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     2941 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     3001 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     3061 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
     3121 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     3181 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     3241 gtgaattgta atacgactca ctatagggcg aattggagct ccaccgcggt ggcggccgcg
     3301 tttataataa acttagtcta atatttcttt tggaccaaaa tagaaactgt tctcaggtgg
     3361 caaaaaacag cttaaagtct gtgttaaaaa aagatctcgg ctatgccaat caaaaaggag
     3421 tgacattatt atgtaaaaga agaaatgttg taattccgcg aaacaacaat tttaatgctt
     3481 actgtgccgc caccaatatc ctatggccaa ggggccttta aacatgccac ggaaatatac
     3541 agtcaaagta aacctactat gtctttagag ccttcaggat caattgtttt taaatgaatg
     3601 actgcaaaac tagaaaagta gcctcttaca taagcaagtc atttgtacac cgagagttta
     3661 gtaaccagtg aagcaagtat atctggcaaa cctgaaactt atctgtgaat tataaagctc
     3721 ataagtaaaa agtgtagaaa agaagataag gcagaccaaa tacaaatttg caacagtaca
     3781 acgaatattt cagcgtcaaa actagagaat aataaagaaa acgatctttt caaaaagaaa
     3841 aaacctttta gttttccttt gttgttgttg tgggtgtgtg ctatttatat tatatagttt
     3901 actcataata ccataaaata ttcggtttga ttaggttatt ttaataagct aatttgtttc
     3961 taatcgtgta atttatgctg tgtatattaa gtagtgtgtg cactgcccaa aaatgtttgt
     4021 tgtttatagt cggttaaaga gaaaaaagaa aaaaagatcc atacacacac gttaattagt
     4081 tgttcaacgt aatacactca tattttgttc ttatttgctt tcggtcgctg ttctcaccaa
     4141 gatttattgc caacgaaaca attttttttt atatattttc agatttttct ttttttcctt
     4201 tcctttcctt ttctaatttt cactcctggt tttctttctt tcttagaaac attatctcga
     4261 tattaatatt aaaaaaatat aatcattcaa actccggaat gtctactact ttggatgata
     4321 ctgcttatag atacagaact tctgttccag gtgatgctga agctattgaa gctttggatg
     4381 gttctttcac taccgatact gtttttagag ttactgctac tggtgatggt ttcactttga
     4441 gagaagttcc agttgatcca ccattgacta aggttttccc agatgatgaa tccgatgatg
     4501 aatccgatgc tggtgaagat ggtgatccag attctagaac tttcgttgct tatggtgatg
     4561 atggtgattt ggctggtttc gttgttgttt cttattctgg ttggaacaga agattgactg
     4621 ttgaagatat tgaagttgct ccagaacata gaggtcatgg tgttggtaga gctttgatgg
     4681 gtttggctac tgaattcgcc agagaaagag gtgctggtca tttgtggttg gaagttacca
     4741 atgttaatgc tccagctatt catgcttata gaagaatggg tttcactttg tgtggtttgg
     4801 atactgcttt atacgatggt actgcttccg atggtgaaca agctttgtat atgtctatgc
     4861 catgtccata ataactcggg gggatccgag tgaaattctg gaaatctgga aatctggttt
     4921 tgtattcttg ttattcttct ttttgttatt acatatataa cttgttactt ttttaaaaaa
     4981 atctttgttt attttataaa tatataaaac taaatttaag aaaaagagaa aaatgtttta
     5041 tttgagagat tgaaatttta cttgaattta gcttagcttt tataaagtat tattatgtaa
     5101 aaaaacaaaa caaatataca ttaaaaagtt aagactataa aatagccacc caaggcattt
     5161 ctatatcttg ttgttgttgt tttcatcttc tgtatcagag gaacttattt tattattttc
     5221 gtcacgggta ttttctcttg tttgatgatt catcccattc ttccatcata aaatactagt
     5281 ggatccccca gatccttatc cttaaacaga ttctaaaaca acatctttga aaccagaaga
     5341 aactttctta cctttttctt cggttgaacc ttcaccgtgc aaagccaata atgaacccaa
     5401 gtcgaatttt ggttgtttca acaatttgac ttttctgatg tggacatttt gtaatgggaa
     5461 aatggtttgg gtggattttt caatttcacg gccaatgact tctggaatca atttggaagt
     5521 taattgagct aaagtacagt tggaaacttc tctttgcata atttcaatca tcttctttct
     5581 gacttctctc aatttagagg attgagcgta agtagttttc ttgatttggt ttggttgtct
     5641 tttggtgaaa gcaatggcaa aaactctcaa aacgtaatcg tcggaagttt taacagtgac
     5701 attagcttca actaatgatt gccattttct gaccaatgat cttaatttgt cagaagtgaa
     5761 atccaaacca tgaggcctcc atgaggcctc catgaggcct ccatgaggcc tccatggagg
     5821 cctcatggaa gttggtcaac aagtttttac cttggacttc atcaactctc aatttgattt
     5881 ttctgtaaga gtggtcttcg gaaccttgta agtcggccaa acaaacttcg aaaactctac
     5941 ctttcaagcc atcagcggca ttctttaaac cggtagatct gttgatcaaa gttttaccaa
     6001 catttctgtt ttcaaaagtg gttggagctt tgatgtcaaa ccaatctttt ctggtgaatg
     6061 ggtcaacgac cttctttttt aatcctttct ttcctttgga caatctcttg tttttaccga
     6121 cagccatgtt gtacttgagt tggatctacg cgtcagcctc gtttataata aacttagtct
     6181 aatatttctt ttggaccaaa atagaaactg ttctcaggtg gcaaaaaaca gcttaaagtc
     6241 tgtgttaaaa aaagatctcg gctatgccaa tcaaaaagga gtgacattat tatgtaaaag
     6301 aagaaatgtt gtaattccgc gaaacaacaa ttttaatgct tactgtgccg ccaccaatat
     6361 cctatggcca aggggccttt aaacatgcca cggaaatata cagtcaaagt aaacctacta
     6421 tgtctttaga gccttcagga tcaattgttt ttaaatgaat gactgcaaaa ctagaaaagt
     6481 agcctcttac ataagcaagt catttgtaca ccgagagttt agtaaccagt gaagcaagta
     6541 tatctggcaa acctgaaact tatctgtgaa ttataaagct cataagtaaa aagtgtagaa
     6601 aagaagataa ggcagaccaa atacaaattt gcaacagtac aacgaatatt tcagcgtcaa
     6661 aactagagaa taataaagaa aacgatcttt tcaaaaagaa aaaacctttt agttttcctt
     6721 tgttgttgtt gtgggtgtgt gctatttata ttatatagtt tactcataat accataaaat
     6781 attcggtttg attaggttat tttaataagc taatttgttt ctaatcgtgt aatttatgct
     6841 gtgtatatta agtagtgtgt gcactgccca aaaatgtttg ttgtttatag tcggttaaga
     6901 gaaaaaagaa aaaaagatcc catacacaca cgttaattag ttgttcaacg taatacactc
     6961 atattttgtt cttatttgct ttcggtcgct gttctcacca agatttattg ccaacgaaac
     7021 aatttttttt tatatatttt cagatttttc tttttttcct ttcctttcct tttctaattt
     7081 tcactcctgg ttttctttct ttcttagaaa cattatctcg atattaatat taaaaaaata
     7141 taatcattca aaatgctgca catgtctaag ggtgaagaat tgtttaccgg tgtggtcccc
     7201 atcttggttg agttagacgg tgatgttaac gggcataagt tcagcgtatc aggtgaaggt
     7261 gaaggtgacg cgacctacgg taagttgact ctaaaattaa tttgtaccac gggtaaattg
     7321 ccagttccat ggccaactct agttaccacg ttgggttacg gtttacagtg ctttgccaga
     7381 tacccagacc acatgaaaca acacgacttc tttaaatctg ccatgccgga aggttacgta
     7441 caagagagaa ccattttctt caaagatgat ggtaattata agacgagagc ggaagtcaag
     7501 ttcgaaggtg atactttggt taacagaatt gaattaaaag gcatcgactt caaagaggac
     7561 gggaacattc taggtcataa gttggaatac aattacaatc tgcataacgt ttacattact
     7621 gccgacaaac aaaaaaacgg cattaaggcc aacttcaaaa ttagacacaa cattgaagat
     7681 gggggtgttc aactagccga ccactaccag caaaatactc caattggtga cgggccagtt
     7741 ttgttgccag acaaccatta cttgtcatat caatccaaat tatcaaagga cccaaacgag
     7801 aaacgcgatc acatggtttt gctagaattt gttaccgctg ccggtattac gcatggtatg
     7861 gatgaattat acaaataa
//
