LOCUS       sequence-31605-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UBE2H
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..21
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..85
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             595..612
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(665..683)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      679..726
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(849..868)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttgtttaact ttaagaagga gatataccat gggcagcagc catcatcatc atcatcacag
       61 cagcggcctg gttccgcgtg gtagtatgtc atctcccagt ccgggcaaga ggcggatgga
      121 cacggacgtg atcaagctca tcgagagtaa acatgaggtt acgatcctgg gaggacttaa
      181 tgaatttgta gtgaagtttt atggaccaca aggaacacca tatgaaggcg gagtatggaa
      241 agttagagtg gacctacctg ataaataccc tttcaaatct ccatctatag gattcatgaa
      301 taaaattttc catcccaaca ttgatgaagc gtcaggaact gtgtgtctag atgtaattaa
      361 tcaaacttgg acagctctct atgatcttac caatatattt gagtccttcc tgcctcagtt
      421 attggcctat cctaacccca tagatcctct caatggtgac gctgcagcca tgtacctcca
      481 ccgaccagaa gaatacaagc agaaaattaa agagtacatc cagaaatacg ccacggagga
      541 ggcgctgaaa gaacaggaag agggttgatg acgaagcttg cggccgcact cgagcaccac
      601 caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
      661 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
      721 tttttgctga aaggaggaac tatatccgga ttggcgaatg gacgcgccct gtagcggcgc
      781 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      841 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      901 tca
//
