LOCUS       sequence-31607-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NRDP1
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             492..509
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(562..580)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      576..623
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(747..766)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggttccgcg tggtagtaca attgaataca acgagatcct agagtgggtg
      121 aactcccttc agccagcaag agtgacccgc tggggaggga tgatctcgac tcctgatgct
      181 gtgctccagg ctgtaatcaa gcgctccctg gtggagagtg gctgtcctgc ttctattgtc
      241 aacgagctga ttgaaaatgc ccacgagcgt agctggcccc agggtctggc cacactagag
      301 actagacaga tgaaccgacg ctactatgag aactacgtgg ccaagcgcat ccctggcaag
      361 caggctgttg tcgtgatggc ctgtgagaac cagcacatgg gggatgacat ggtgcaagag
      421 ccaggccttg tcatgatatt tgcacatggc gtggaagaga tatgatgacg aagcttgcgg
      481 ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg
      541 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
      601 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga
      661 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
      721 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
      781 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
      841 tgctttacgg cacctcgacc ccaaaaactt gattagggtg atggttcacg tagtgggcca
      901 tcgccctgat agac
//
