LOCUS       sequence-316071-         965 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral CaTCH barcoding plasmid harboring a single barcode 
            cassette (BC1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Umkehrer C, Holstein F, Formenti L, Jude J, Froussios K, Neumann T, 
            Cronin SM, Haas L, Lipp JJ, Burkard TR, Fellner M, Wiesner T, Zuber 
            J, Obenauf AC
  TITLE     Isolating live cell clones from barcoded populations using 
            CRISPRa-inducible reporters.
  JOURNAL   Nat Biotechnol. 2020 Jul 27. pii: 10.1038/s41587-020-0614-0. doi: 
            10.1038/s41587-020-0614-0.
  PUBMED    32719478
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Jul 27. pii: 10.1038/s41587-020-0614-0. doi: 
            10.1038/s41587-020-0614-0."
COMMENT     Sequence Label: CaTCH BC1
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    533..766
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ggatatcact agtcgccgcc atttgcgcgc gctcgcccgt tcgctctaga cagggtaccc
       61 agcatatgat agggtcccct cggggttggg aggtgggtct gaaacgataa tggtgaatat
      121 ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa acctaccaag
      181 cctcctacta tcattatgaa taattttata taccacagcc aatttgttat gttaaaccaa
      241 ttccacaaac ttgcccattt atctaattcc aatagatcgg aagagcacac gtctgaactc
      301 cagtcacatc tcgtatgccg tcttctgctt gaattcttgt tcattctttt cttgctggtt
      361 ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca
      421 ctccatccag gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt
      481 ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg
      541 ttgatccttt aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca
      601 gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg
      661 ctgcactata ccagacaata attgtctggn ctgtaccgtc agcgtcattg acgctgcgcc
      721 catagtgctt cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct
      781 tttttctctc tgcaccactc ttctctttgc cttggtgggt gctactccta atggntcaat
      841 ttttactact ttatatttat ataattcact tctccnnttg tccctcatat ctcctcctcc
      901 aggtctgaag atcagcngcc gcttgctgtg cgnnggtctt acttttgttt tgctcttcct
      961 ctatc
//
