LOCUS       sequence-316073-        5720 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0220:Dendra
FEATURES             Location/Qualifiers
     source          1..5720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1664..1687
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1944..2744
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2869..3457
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3358..3377
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tggtgagcag cacccgcagt gaacacagct ttgccggcgt
      121 cggcggcgtc cgcatcgtct acgacgtgtg gacccccgac accgacccgc gcggggtcgt
      181 cgtgctggcg cacggttacg ccgaacacgc aggccgctac caccacgtcg cgcaacggtt
      241 cggggccgcg ggcctgctcg tgtacgcact cgaccaccgc ggacacggcc gctccggcgg
      301 taagcgcgtt cacctgcgcg acctgtcgga gttcgtcgag gacttccgca cactcgtcgg
      361 catcgccgcg aacgaccacc ccacactgcc gcggatcgtg ctcggccaca gcatgggcgg
      421 cggcatcgtc ttcgcctatg gcgctcggta cccgggcgag tactcggcca tggtgctgtc
      481 cgggcccgcc gtgaacgcac acgacggcgt atcgccggtg ctggtcgcgg tggccaaagt
      541 gctgggcaaa ctcgcgcccg gcatcccggt ggagaacctg gacgccgacg cggtctcgcg
      601 cgaccccgag gtggtcgcgg cctacaaggc cgatccgatg gttcaccacg gcaagctgcc
      661 cgcgggcatc gcgcgcgcgc tgatcggcct gggacagagc atgccgcagc gggccgcggc
      721 gctgaccgcg ccgctgctgg tggtgcacgg cgacaaggac cgcctcatcc cggtggcggg
      781 cagccggctg ctcgtcgacc gcgtggcttc cgaggatgtc cacctgaagg tctaccccgg
      841 gctgtaccac gaggtgttca acgaacccga acagaaactc gtcctcgacg acgtcacctc
      901 atggatcgtg agtcatctga agcttggtgc ggccgcgggc aacacgcctg gcatcaatct
      961 gatcaaggag gacatgcgtg tcaaggtgca tatggagggc aacgtgaacg gtcacgcctt
     1021 cgtgatcgag ggggagggta agggtaagcc ttacgaaggg acccagaccg ccaacctcac
     1081 cgtcaaggag ggcgcccctc tcccattctc gtacgatatc ctcactaccg cggtgcatta
     1141 cgggaaccgc gtgttcacca agtaccccga ggatatccct gactacttca agcagagctt
     1201 cccggaggga tactcctggg agcggacgat gaccttcgaa gacaagggta tctgtacgat
     1261 ccgcagcgac atctcgctgg aaggggactg tttcttccag aacgtccgtt tcaaggggac
     1321 caactttccg ccgaacggcc cggtcatgca gaagaagacc ctcaagtggg agccgagcac
     1381 ggagaagctg cacgttcggg acggcctcct ggtcggcaac atcaacatgg cgctgctgct
     1441 ggaaggcggc ggccactacc tatgcgactt caagaccacg tacaaagcga agaaggtcgt
     1501 ccagctgccc gacgcgcact tcgtggacca ccgcatcgaa atcctgggga acgattcgga
     1561 ctacaacaag gtcaagttgt acgagcacgc tgtggcacgc tatagcccgc tcccgtcgca
     1621 ggtctggttc gaaggcggcg gcggggagaa cctgtatttc caggactaca aggacgacga
     1681 cgacaagtga tttaaataac cccttggggc ctctaaacgg gtcttgaggg gttttttgtt
     1741 aactagcgta cgatcgaatt cctgcagctc acggtaactg atgccgtatt tgcagtacca
     1801 gcgtacggcc cacagaatga tgtcacgctg aaaatgccgg cctttgaatg ggttcatgtg
     1861 cagctccatc agcaaaaggg gatgataagt ttatcaccac cgactatttg caacagtgcc
     1921 gttgatcgtg ctatgatcga ctgatgtcat cagcggtgga gtgcaatgtc gtgcaatacg
     1981 aatggcgaaa agccgagctc atcggtcagc ttctcaacct tggggttacc cccggcggtg
     2041 tgctgctggt ccacagctcc ttccgtagcg tccggcccct cgaagatggg ccacttggac
     2101 tgatcgaggc cctgcgtgct acgctgggtc cgggagggac gctcgtcatg ccctcgtggt
     2161 caggtctgga cgacgagccg ttcgatcctg ccacgtcgcc cgttacaccg gaccttggag
     2221 ttgtctctga cacattctgg cgcctgccaa atgtaaagcg cagcgcccat ccatttgcct
     2281 ttgcggcagc ggggccacag gcagagcaga tcatctctga tccattgccc ctgccacctt
     2341 actcgcctgc aagcccggtc gcccgtgtcc atgaactcga tgggcaggta cttctcctcg
     2401 gcgtgggaca cgatgccaac acgacgctgc atcttgccga gttgatggca aaggttccct
     2461 atggggtgcc gagacactgc accattcttc aggatggcaa gttggtacgc gtcgattatc
     2521 tcgagaatga ccactgctgt gagcgctttg ccttggcgga caggtggctc aaggagaaga
     2581 gccttcagaa ggaaggtcca gtcggtcatg cctttgctcg gttgatccgc tcccgcgaca
     2641 ttgtggcgac agccctgggt caactgggcc gagatccgtt gatcttcctg catccgccag
     2701 aggcgggatg cgaagaatgc gatgccgctc gccagtcgat tggctgagct catgagcgga
     2761 gaacgagatg acgttggagg ggcaaggtcg cgctgattgc tggggcaaca cgggggatcc
     2821 actagttcca tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     2881 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     2941 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3001 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3061 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3121 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3181 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3241 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgagggga gaaaggcgga
     3301 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3361 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3421 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     3481 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     3541 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     3601 gaccgagcgc aacgcgtgcg gccgcggtac ccggggatcc tctagagtcg accaccaagg
     3661 gcaccatctc tgcttgggcc accccgttgg ccgcagccag ctcgctgaga gccgtgaacg
     3721 acagggcgaa cgccagcccg ccgacggcga gggttccgac cgctgcaact cccggtgcaa
     3781 ccttgtcccg gtctattctc ttcactgcac cagctccaat ctggtgtgaa tgcccctcgt
     3841 ctgttcgcgc aggcgggggg ctctattcgt ttgtcagcat cgaaagtagc cagatcaggg
     3901 atgcgttgca accgcgtatg cccaggtcag aagagtcgca caagagttgc agacccctgg
     3961 aaagaaaaat ggccagaggg cgaaaacacc ctctgaccag cggagcgggc gacgggaatc
     4021 gaacccgcgt agctagtttg gaagaatggg tgtctgccga ccacatatgg gccggtcaag
     4081 ataggttttt accccctctc ggctgcatcc tctaagtgga aagaaattgc aggtcgtaga
     4141 agcgcgttga agcctgagag ttgcacagga gttgcaaccc ggtagccttg ttcacgacga
     4201 gaggagacct agttggcacg tcgcggatgg ggatcgctga agactcagcg cagcgggagg
     4261 atccaagcct catacgtcaa cccgcaggac ggtgtgaggt actacgcgct gcagacctac
     4321 gacaacaaga tggacgccga agcctggctc gcgggcgaga agcggctcat cgagatggag
     4381 acctggaccc ctccacagga ccgggcgaag aaggcagccg ccagcgccat cacgctggag
     4441 gagtacaccc ggaagtggct cgtggagcgc gacctcgcag acggcaccag ggatctgtac
     4501 agcgggcacg cggagcgccg catctacccg gtgctaggtg aagtggcggt cacagagatg
     4561 acgccagctc tggtgcgtgc gtggtgggcc gggatgggta ggaagcaccc gactgcccgc
     4621 cggcatgcct acaacgtcct ccgggcggtg atgaacacag cggtcgagga caagctgatc
     4681 gcagagaacc cgtgccggat cgagcagaag gcagccgatg agcgcgacgt agaggcgctg
     4741 acgcctgagg agctggacat cgtcgccgct gagatcttcg agcactaccg gatcgcggca
     4801 tacatcctgg cgtggacgag cctccggttc ggagagctga tcgagcttcg ccgcaaggac
     4861 atcgtggacg acggcatgac gatgaagctc cgggtgcgcc gtggcgcttc ccgcgtgggg
     4921 aacaagatcg tcgttggcaa cgccaagacc gtccggtcga agcgtcctgt gacggttccg
     4981 cctcacgtcg cggagatgat ccgagcgcac atgaaggacc gtacgaagat gaacaagggc
     5041 cccgaggcat tcctggtgac cacgacgcag ggcaaccggc tgtcgaagtc cgcgttcacc
     5101 aagtcgctga agcgtggcta cgccaagatc ggtcggccgg aactccgcat ccacgacctc
     5161 cgcgctgtcg gcgctacgtt cgccgctcag gcaggtgcga cgaccaagga gctgatggcc
     5221 cgtctcggtc acacgactcc taggatggcg atgaagtacc agatggcgtc tgaggcccgc
     5281 gacgaggcta tcgctgaggc gatgtccaag ctggccaaga cctcctgaaa cgcaaaaagc
     5341 ccccctccca aggacactga gtcctaaaga ggggggtttc ttgtcagtac gcgaagaacc
     5401 acgcctggcc gcgagcgcca gcaccgccgc tctgtgcgga gacctgggca ccagccccgc
     5461 cgccgccagg agcattgccg ttcccgccag aaatctagac ggtgaccaca acgcgcccgc
     5521 tttgatcggg gacgtcgaaa tattggatcg tcggcaccgt cacggccgtg ggaggcggca
     5581 cgatccgcga cgtgatgatc ggccgcatcc ccacggtgct gcgcagtgag ctctacgcca
     5641 tcccggcgtt gatctgtgcg ttcgcacgca caggcccggt gtgagaaggg tctctgacga
     5701 gcgggagaac tccctatcag
//
