LOCUS       sequence-316075-        5576 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0251:Dendra
FEATURES             Location/Qualifiers
     source          1..5576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1520..1543
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1800..2600
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2725..3313
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3214..3233
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgacgcccga catcgtggag gacctccagc aagaatcctc
      121 acccgaaacc gatgcggttc cgggtgtccg acgggtcctg ctggtctggg acgcaccgaa
      181 tctggacatg ggtctcgggt cgattctcgg cggcaggccc acagccgcgc accgcccgcg
      241 gttcgacgcg ctgggccggt ggcttctggc ctataccgcc gagctggccg acggtgtcgg
      301 cgcggaggaa accgcgatct cccatgagcc cgaggcgacg gtcttcacca acatcgcgcc
      361 aggtagcgcc gatgtcgtgc gcccgtgggt cgaggcgttg cgtaacgtgg gtttcgccgt
      421 cttcgccaaa ccgaagatcg atgaagacag cgatgtcgac agcgacatgc tcgaccacat
      481 cgcactgcgc agacgtgagg ggctggcggc ggtactggtc gcctccgcgg acgggcaggc
      541 gttccggcaa ccgctggagg aaatcgcccg tgagggcacg cctgtgcagg ttctcggatt
      601 tcgcgaacat gccagctggg cgctagcgtc ggataccttg gagtttgtcg atctggagga
      661 tattcctggt gttttccggg aaccgctgcc acggatcggc ctggactcgc tacccgagca
      721 gggggcgtgg ctgcagccgt tccggccgtt gtcgtcgcta ctgacctcgc gtgtgaagct
      781 tggtgcggcc gcgggcaaca cgcctggcat caatctgatc aaggaggaca tgcgtgtcaa
      841 ggtgcatatg gagggcaacg tgaacggtca cgccttcgtg atcgaggggg agggtaaggg
      901 taagccttac gaagggaccc agaccgccaa cctcaccgtc aaggagggcg cccctctccc
      961 attctcgtac gatatcctca ctaccgcggt gcattacggg aaccgcgtgt tcaccaagta
     1021 ccccgaggat atccctgact acttcaagca gagcttcccg gagggatact cctgggagcg
     1081 gacgatgacc ttcgaagaca agggtatctg tacgatccgc agcgacatct cgctggaagg
     1141 ggactgtttc ttccagaacg tccgtttcaa ggggaccaac tttccgccga acggcccggt
     1201 catgcagaag aagaccctca agtgggagcc gagcacggag aagctgcacg ttcgggacgg
     1261 cctcctggtc ggcaacatca acatggcgct gctgctggaa ggcggcggcc actacctatg
     1321 cgacttcaag accacgtaca aagcgaagaa ggtcgtccag ctgcccgacg cgcacttcgt
     1381 ggaccaccgc atcgaaatcc tggggaacga ttcggactac aacaaggtca agttgtacga
     1441 gcacgctgtg gcacgctata gcccgctccc gtcgcaggtc tggttcgaag gcggcggcgg
     1501 ggagaacctg tatttccagg actacaagga cgacgacgac aagtgattta aataacccct
     1561 tggggcctct aaacgggtct tgaggggttt tttgttaact agcgtacgat cgaattcctg
     1621 cagctcacgg taactgatgc cgtatttgca gtaccagcgt acggcccaca gaatgatgtc
     1681 acgctgaaaa tgccggcctt tgaatgggtt catgtgcagc tccatcagca aaaggggatg
     1741 ataagtttat caccaccgac tatttgcaac agtgccgttg atcgtgctat gatcgactga
     1801 tgtcatcagc ggtggagtgc aatgtcgtgc aatacgaatg gcgaaaagcc gagctcatcg
     1861 gtcagcttct caaccttggg gttacccccg gcggtgtgct gctggtccac agctccttcc
     1921 gtagcgtccg gcccctcgaa gatgggccac ttggactgat cgaggccctg cgtgctacgc
     1981 tgggtccggg agggacgctc gtcatgccct cgtggtcagg tctggacgac gagccgttcg
     2041 atcctgccac gtcgcccgtt acaccggacc ttggagttgt ctctgacaca ttctggcgcc
     2101 tgccaaatgt aaagcgcagc gcccatccat ttgcctttgc ggcagcgggg ccacaggcag
     2161 agcagatcat ctctgatcca ttgcccctgc caccttactc gcctgcaagc ccggtcgccc
     2221 gtgtccatga actcgatggg caggtacttc tcctcggcgt gggacacgat gccaacacga
     2281 cgctgcatct tgccgagttg atggcaaagg ttccctatgg ggtgccgaga cactgcacca
     2341 ttcttcagga tggcaagttg gtacgcgtcg attatctcga gaatgaccac tgctgtgagc
     2401 gctttgcctt ggcggacagg tggctcaagg agaagagcct tcagaaggaa ggtccagtcg
     2461 gtcatgcctt tgctcggttg atccgctccc gcgacattgt ggcgacagcc ctgggtcaac
     2521 tgggccgaga tccgttgatc ttcctgcatc cgccagaggc gggatgcgaa gaatgcgatg
     2581 ccgctcgcca gtcgattggc tgagctcatg agcggagaac gagatgacgt tggaggggca
     2641 aggtcgcgct gattgctggg gcaacacggg ggatccacta gttccatgag cgtcagaccc
     2701 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2761 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2821 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     2881 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2941 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     3001 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     3061 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     3121 agaaagcgcc acgcttcccg aggggagaaa ggcggacagg tatccggtaa gcggcagggt
     3181 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     3241 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     3301 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     3361 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     3421 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcaacg cgtgcggccg
     3481 cggtacccgg ggatcctcta gagtcgacca ccaagggcac catctctgct tgggccaccc
     3541 cgttggccgc agccagctcg ctgagagccg tgaacgacag ggcgaacgcc agcccgccga
     3601 cggcgagggt tccgaccgct gcaactcccg gtgcaacctt gtcccggtct attctcttca
     3661 ctgcaccagc tccaatctgg tgtgaatgcc cctcgtctgt tcgcgcaggc ggggggctct
     3721 attcgtttgt cagcatcgaa agtagccaga tcagggatgc gttgcaaccg cgtatgccca
     3781 ggtcagaaga gtcgcacaag agttgcagac ccctggaaag aaaaatggcc agagggcgaa
     3841 aacaccctct gaccagcgga gcgggcgacg ggaatcgaac ccgcgtagct agtttggaag
     3901 aatgggtgtc tgccgaccac atatgggccg gtcaagatag gtttttaccc cctctcggct
     3961 gcatcctcta agtggaaaga aattgcaggt cgtagaagcg cgttgaagcc tgagagttgc
     4021 acaggagttg caacccggta gccttgttca cgacgagagg agacctagtt ggcacgtcgc
     4081 ggatggggat cgctgaagac tcagcgcagc gggaggatcc aagcctcata cgtcaacccg
     4141 caggacggtg tgaggtacta cgcgctgcag acctacgaca acaagatgga cgccgaagcc
     4201 tggctcgcgg gcgagaagcg gctcatcgag atggagacct ggacccctcc acaggaccgg
     4261 gcgaagaagg cagccgccag cgccatcacg ctggaggagt acacccggaa gtggctcgtg
     4321 gagcgcgacc tcgcagacgg caccagggat ctgtacagcg ggcacgcgga gcgccgcatc
     4381 tacccggtgc taggtgaagt ggcggtcaca gagatgacgc cagctctggt gcgtgcgtgg
     4441 tgggccggga tgggtaggaa gcacccgact gcccgccggc atgcctacaa cgtcctccgg
     4501 gcggtgatga acacagcggt cgaggacaag ctgatcgcag agaacccgtg ccggatcgag
     4561 cagaaggcag ccgatgagcg cgacgtagag gcgctgacgc ctgaggagct ggacatcgtc
     4621 gccgctgaga tcttcgagca ctaccggatc gcggcataca tcctggcgtg gacgagcctc
     4681 cggttcggag agctgatcga gcttcgccgc aaggacatcg tggacgacgg catgacgatg
     4741 aagctccggg tgcgccgtgg cgcttcccgc gtggggaaca agatcgtcgt tggcaacgcc
     4801 aagaccgtcc ggtcgaagcg tcctgtgacg gttccgcctc acgtcgcgga gatgatccga
     4861 gcgcacatga aggaccgtac gaagatgaac aagggccccg aggcattcct ggtgaccacg
     4921 acgcagggca accggctgtc gaagtccgcg ttcaccaagt cgctgaagcg tggctacgcc
     4981 aagatcggtc ggccggaact ccgcatccac gacctccgcg ctgtcggcgc tacgttcgcc
     5041 gctcaggcag gtgcgacgac caaggagctg atggcccgtc tcggtcacac gactcctagg
     5101 atggcgatga agtaccagat ggcgtctgag gcccgcgacg aggctatcgc tgaggcgatg
     5161 tccaagctgg ccaagacctc ctgaaacgca aaaagccccc ctcccaagga cactgagtcc
     5221 taaagagggg ggtttcttgt cagtacgcga agaaccacgc ctggccgcga gcgccagcac
     5281 cgccgctctg tgcggagacc tgggcaccag ccccgccgcc gccaggagca ttgccgttcc
     5341 cgccagaaat ctagacggtg accacaacgc gcccgctttg atcggggacg tcgaaatatt
     5401 ggatcgtcgg caccgtcacg gccgtgggag gcggcacgat ccgcgacgtg atgatcggcc
     5461 gcatccccac ggtgctgcgc agtgagctct acgccatccc ggcgttgatc tgtgcgttcg
     5521 cacgcacagg cccggtgtga gaagggtctc tgacgagcgg gagaactccc tatcag
//
