LOCUS       sequence-316077-        6299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0409:Dendra
FEATURES             Location/Qualifiers
     source          1..6299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2243..2266
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2523..3323
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3448..4036
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3937..3956
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgcgaatcgg caagatcacg gttggcccac tcgatgaatg
      121 gtcgctcaag ccgggcacgg tcacttcctg gcaaccgacg gccgccgcgc gcgaagtggc
      181 gcgacaggcg ccggtcagtt cggttcccgt gagctacatg caagagcaac atctgcgtaa
      241 cttctgcgaa cgcaccgccg cgggattgaa tttctccaga cagatcatcg cgagttgtga
      301 agtaccgggc caatgcgaca tttccgcgat ggatcacgcc gtcaactcat acctgcgaag
      361 gcacgacacg ttccgcagtt ggttcgagga cgcgggcgac gggaaattca tccggcattc
      421 catcgagaac ccggacgaca tcgaattcgt tcctgtcgaa cacggtgcga tgtcgatcga
      481 cgagatccac gcccatgtga ccgacatacc gactccgttg gagtgggcat gcttcacgtt
      541 cggggtcatc cagagcgagg accatttcac gttcttcgcg gccatggacc acgtccacgg
      601 cgacgcgacc ctgatcggca cgacaatgct cgaagccaac ggcatgtatt cggcgctcag
      661 cggcggcggc gaggcgctgg ttcttcccga agcgggcagc ttcgacgatt tctgtattcg
      721 agaacgtgaa cgcaccgcgg atctgaccgt cgactcgccc gaggtccgcg cctggatcga
      781 attcgcggaa gacaacaatg gcggctttcc cgagttcccg cttccgctgg ggaatccgaa
      841 gaaatcgacc agtagcgaca tgacgtcgga aatcctgctg gaccccgcgc agacggaacg
      901 tttcgaatcg gcatgcaacg cggccggggc gcgttttgtc ggtggtcttt tcgcgtgcct
      961 ggcgcaggtg gaacacgagt tgaccggtgc actcacgtat tacgggctca ctccgcgtga
     1021 ctcgagaaat gccaccgaca atttcatgac gcagggctgg ttcacgggtc tcatccctat
     1081 caccgttccg atcggtgcgg cgtcattcac cgaagccgcc ggatccgccc agagatcttt
     1141 cgacgccaat ctgaacatgg cgaaagtccc gtattaccgg gtgctggagt tggccccgtc
     1201 gctgcggtgg ccgcaaccca atttccctgt gtcgaatttc ttccacggtg gcgccgcgcc
     1261 actcaacgcc gttctcgccg cggccgacat gggactcgcg aacaacatcg ggatctaccc
     1321 cgacggccgc tactcgtatc agctcaccat ctacatattc cggtatggcg agggcaccgc
     1381 catggcaatc atgcatcccg acaattcagt cgccagaaag tcggccaccc gctacatgga
     1441 gacgatgcgc tcggtagccg gtcaggtggc cgacagtggt aactgggggc gcgtcgcaaa
     1501 gcttggtgcg gccgcgggca acacgcctgg catcaatctg atcaaggagg acatgcgtgt
     1561 caaggtgcat atggagggca acgtgaacgg tcacgccttc gtgatcgagg gggagggtaa
     1621 gggtaagcct tacgaaggga cccagaccgc caacctcacc gtcaaggagg gcgcccctct
     1681 cccattctcg tacgatatcc tcactaccgc ggtgcattac gggaaccgcg tgttcaccaa
     1741 gtaccccgag gatatccctg actacttcaa gcagagcttc ccggagggat actcctggga
     1801 gcggacgatg accttcgaag acaagggtat ctgtacgatc cgcagcgaca tctcgctgga
     1861 aggggactgt ttcttccaga acgtccgttt caaggggacc aactttccgc cgaacggccc
     1921 ggtcatgcag aagaagaccc tcaagtggga gccgagcacg gagaagctgc acgttcggga
     1981 cggcctcctg gtcggcaaca tcaacatggc gctgctgctg gaaggcggcg gccactacct
     2041 atgcgacttc aagaccacgt acaaagcgaa gaaggtcgtc cagctgcccg acgcgcactt
     2101 cgtggaccac cgcatcgaaa tcctggggaa cgattcggac tacaacaagg tcaagttgta
     2161 cgagcacgct gtggcacgct atagcccgct cccgtcgcag gtctggttcg aaggcggcgg
     2221 cggggagaac ctgtatttcc aggactacaa ggacgacgac gacaagtgat ttaaataacc
     2281 ccttggggcc tctaaacggg tcttgagggg ttttttgtta actagcgtac gatcgaattc
     2341 ctgcagctca cggtaactga tgccgtattt gcagtaccag cgtacggccc acagaatgat
     2401 gtcacgctga aaatgccggc ctttgaatgg gttcatgtgc agctccatca gcaaaagggg
     2461 atgataagtt tatcaccacc gactatttgc aacagtgccg ttgatcgtgc tatgatcgac
     2521 tgatgtcatc agcggtggag tgcaatgtcg tgcaatacga atggcgaaaa gccgagctca
     2581 tcggtcagct tctcaacctt ggggttaccc ccggcggtgt gctgctggtc cacagctcct
     2641 tccgtagcgt ccggcccctc gaagatgggc cacttggact gatcgaggcc ctgcgtgcta
     2701 cgctgggtcc gggagggacg ctcgtcatgc cctcgtggtc aggtctggac gacgagccgt
     2761 tcgatcctgc cacgtcgccc gttacaccgg accttggagt tgtctctgac acattctggc
     2821 gcctgccaaa tgtaaagcgc agcgcccatc catttgcctt tgcggcagcg gggccacagg
     2881 cagagcagat catctctgat ccattgcccc tgccacctta ctcgcctgca agcccggtcg
     2941 cccgtgtcca tgaactcgat gggcaggtac ttctcctcgg cgtgggacac gatgccaaca
     3001 cgacgctgca tcttgccgag ttgatggcaa aggttcccta tggggtgccg agacactgca
     3061 ccattcttca ggatggcaag ttggtacgcg tcgattatct cgagaatgac cactgctgtg
     3121 agcgctttgc cttggcggac aggtggctca aggagaagag ccttcagaag gaaggtccag
     3181 tcggtcatgc ctttgctcgg ttgatccgct cccgcgacat tgtggcgaca gccctgggtc
     3241 aactgggccg agatccgttg atcttcctgc atccgccaga ggcgggatgc gaagaatgcg
     3301 atgccgctcg ccagtcgatt ggctgagctc atgagcggag aacgagatga cgttggaggg
     3361 gcaaggtcgc gctgattgct ggggcaacac gggggatcca ctagttccat gagcgtcaga
     3421 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3481 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3541 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     3601 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3661 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3721 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3781 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3841 atgagaaagc gccacgcttc ccgaggggag aaaggcggac aggtatccgg taagcggcag
     3901 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3961 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4021 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4081 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     4141 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca acgcgtgcgg
     4201 ccgcggtacc cggggatcct ctagagtcga ccaccaaggg caccatctct gcttgggcca
     4261 ccccgttggc cgcagccagc tcgctgagag ccgtgaacga cagggcgaac gccagcccgc
     4321 cgacggcgag ggttccgacc gctgcaactc ccggtgcaac cttgtcccgg tctattctct
     4381 tcactgcacc agctccaatc tggtgtgaat gcccctcgtc tgttcgcgca ggcggggggc
     4441 tctattcgtt tgtcagcatc gaaagtagcc agatcaggga tgcgttgcaa ccgcgtatgc
     4501 ccaggtcaga agagtcgcac aagagttgca gacccctgga aagaaaaatg gccagagggc
     4561 gaaaacaccc tctgaccagc ggagcgggcg acgggaatcg aacccgcgta gctagtttgg
     4621 aagaatgggt gtctgccgac cacatatggg ccggtcaaga taggttttta ccccctctcg
     4681 gctgcatcct ctaagtggaa agaaattgca ggtcgtagaa gcgcgttgaa gcctgagagt
     4741 tgcacaggag ttgcaacccg gtagccttgt tcacgacgag aggagaccta gttggcacgt
     4801 cgcggatggg gatcgctgaa gactcagcgc agcgggagga tccaagcctc atacgtcaac
     4861 ccgcaggacg gtgtgaggta ctacgcgctg cagacctacg acaacaagat ggacgccgaa
     4921 gcctggctcg cgggcgagaa gcggctcatc gagatggaga cctggacccc tccacaggac
     4981 cgggcgaaga aggcagccgc cagcgccatc acgctggagg agtacacccg gaagtggctc
     5041 gtggagcgcg acctcgcaga cggcaccagg gatctgtaca gcgggcacgc ggagcgccgc
     5101 atctacccgg tgctaggtga agtggcggtc acagagatga cgccagctct ggtgcgtgcg
     5161 tggtgggccg ggatgggtag gaagcacccg actgcccgcc ggcatgccta caacgtcctc
     5221 cgggcggtga tgaacacagc ggtcgaggac aagctgatcg cagagaaccc gtgccggatc
     5281 gagcagaagg cagccgatga gcgcgacgta gaggcgctga cgcctgagga gctggacatc
     5341 gtcgccgctg agatcttcga gcactaccgg atcgcggcat acatcctggc gtggacgagc
     5401 ctccggttcg gagagctgat cgagcttcgc cgcaaggaca tcgtggacga cggcatgacg
     5461 atgaagctcc gggtgcgccg tggcgcttcc cgcgtgggga acaagatcgt cgttggcaac
     5521 gccaagaccg tccggtcgaa gcgtcctgtg acggttccgc ctcacgtcgc ggagatgatc
     5581 cgagcgcaca tgaaggaccg tacgaagatg aacaagggcc ccgaggcatt cctggtgacc
     5641 acgacgcagg gcaaccggct gtcgaagtcc gcgttcacca agtcgctgaa gcgtggctac
     5701 gccaagatcg gtcggccgga actccgcatc cacgacctcc gcgctgtcgg cgctacgttc
     5761 gccgctcagg caggtgcgac gaccaaggag ctgatggccc gtctcggtca cacgactcct
     5821 aggatggcga tgaagtacca gatggcgtct gaggcccgcg acgaggctat cgctgaggcg
     5881 atgtccaagc tggccaagac ctcctgaaac gcaaaaagcc cccctcccaa ggacactgag
     5941 tcctaaagag gggggtttct tgtcagtacg cgaagaacca cgcctggccg cgagcgccag
     6001 caccgccgct ctgtgcggag acctgggcac cagccccgcc gccgccagga gcattgccgt
     6061 tcccgccaga aatctagacg gtgaccacaa cgcgcccgct ttgatcgggg acgtcgaaat
     6121 attggatcgt cggcaccgtc acggccgtgg gaggcggcac gatccgcgac gtgatgatcg
     6181 gccgcatccc cacggtgctg cgcagtgagc tctacgccat cccggcgttg atctgtgcgt
     6241 tcgcacgcac aggcccggtg tgagaagggt ctctgacgag cgggagaact ccctatcag
//
