LOCUS       sequence-316078-        8006 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 8006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0690:Dendra
FEATURES             Location/Qualifiers
     source          1..8006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3950..3973
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4230..5030
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      5155..5743
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5644..5663
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tggcacacac cctcgaagtg agcaggctca tcatcgggct
      121 gctcatgacg gccatcgtct tggtgttcgc cgccaagcga gtgctctggc tgacgaagct
      181 gattcgctcg ggccagaaga cgctcgacga gaacggccgc aagaacgatc tgcaaaagcg
      241 catcaccacg cagatcaccg aggtcttcgg gcaaacgcgc ctgctgcgct ggtcggttcc
      301 gggcatcgcg cacttcttca cgatgtgggg cttcttcgtc ctcgcctcgg tgtacctcga
      361 ggcctacggc gtgctgttcg atcccgagtt ccacatcccg ttcatcggcc gctggccggt
      421 cctgggcttt ctgcaggact tcttcgccgt cgccgtgctg ctgggcatca tcgtcttcgc
      481 gatcatccgc gtcgtccgtg agcccaagaa gatcgggcgc gactcgcgct tctacggttc
      541 gcacaccggc ggcgcgtggg agatcctgtt catgatcttc ctggtcatcg cgacctacgc
      601 gctgttccgc ggtgccgcgg tcaacaccct cggcgagcgt ttcccctacc agagcggcgc
      661 cttcttctcc gacttcatgg cgtggatcct gcgcccgctc ggcgcgaccg ccaacatgtg
      721 gatcgagacc gtcgccctga tgggccacat cggcgtcatg ctggtgttcc tgctgatcgt
      781 gctgcactcc aagcacctgc acatcggcct tgcgcccatc aacgtcacgt tcaagcgcct
      841 gcccaacggc ctcggcccgc tgctgccgat ggagtccaac ggcgagtaca tcgacttcga
      901 ggatcccgcc gaggacgcgg tgttcggtaa aggcaagatc gaggacttca cctggaaggg
      961 ttacctggac ttcacgacct gtaccgagtg tggccgctgc cagtcgcagt gcccggcgtg
     1021 gaacaccggc aaaccgctgt cgcccaagct cgtgatcatg aacctgcgcg accacatgtt
     1081 cgccaaggct ccctacatcc tgggcgagaa gccgtcgccg ctggagagca cgcccgaggg
     1141 cggcctgggt gagaaggccc gcggcgagaa gcacgagcag aagcacgcgc acgaccacgt
     1201 ccccgagtcc ggcttcgagc ggatcctcgg cagcggcccc gagcaggccc tgcgcccgct
     1261 ggtcggcacc gaggaacagg gcggcgtgat cgatcccgac gtgctgtggt cctgcaccaa
     1321 ctgcggtgcg tgcgtcgagc agtgccccgt ggacatcgag cacatcgacc acatcgtcga
     1381 catgcgccgc taccaggtca tggtcgagtc ggagttcccc ggtgagctgg gcgtgctgtt
     1441 caagaacctg gagaccaagg gcaacccctg gggccagaac gccaaggacc gcaccaactg
     1501 gatcgacgag gtcgacttcg acgtgccggt ctacggcgag gacgtcgact cgttcgacgg
     1561 gttcgagtac ctgttctggg tcggctgcgc cggcgcctac gaggaccgcg ccaagaagac
     1621 cacaaaggcc gtcgccgaac tgctcgccac cgcgggcgtg aagttcctgg tgctgggcac
     1681 cggcgagacc tgcacgggcg actcggcgcg ccgctcgggc aacgagttcc tgttccagca
     1741 gctggccgcg cagaacgtcg agaccatcaa cgaactgttc gaaggcgtcg agacggtcga
     1801 ccgcaagatc gtggtgacgt gcccgcactg cttcaacacg atcggccgtg aatacccgca
     1861 gctgggtgcc aactacagcg tcgtgcacca cacgcagctg ctcaaccggc tggtccgcga
     1921 caagaagctg gttccggtca agtcggtcag cgagcagaac ggccagcccg tcacctacca
     1981 cgacccgtgc ttcctgggcc gccacaacaa ggtctacgag gccccgcgtg agctggtcga
     2041 ggcctccggc gtgacgctga aggaaatgcc gcgccacgcc gaccgcggcc tgtgctgtgg
     2101 cgccggcggt gcgcgtatgt ggatggaaga gcacatcggc aagcgcgtca acgtcgaacg
     2161 caccgaagag gccatggaca cggcctcgac catcgcgacc ggctgcccgt tctgccgcgt
     2221 gatgatcacc gacggtgtcg acgacgtggc ggccagccgc aacgtcgaga aggccgaggt
     2281 gctcgacgtc gcccagctgc tgctgaactc gctggacacc agcaaggtca cgctgcccga
     2341 aaagggcacg gcggcaaagg aatccgagaa gcgcgccgct gcccgggcag aggccgaagc
     2401 gaaggcggaa gcggccgctc ccccggtcga ggaagccgca cccgaagcag aggccccggc
     2461 cgccccggca gccggtgggg ccgaggccaa gccggtcacc ggcctgggca tggcgggtgc
     2521 cgcgaagcgt ccgggcgcca agaaagccgc acctgccgcg gaagcgtctg cggcaccggc
     2581 cgccgcgccc gccccggcca agggtctggg cctggccggc ggggccaagc gtcccggcgc
     2641 gaagaaggcc gccgcaccgg ccgccgaggc acccgctgct ccggcttcgg acgctccgcc
     2701 ggtcaagggg ctcggtctcg cgggtggtgc caagcgaccc ggcgccaaga agaccgcggc
     2761 agctgctccg gccgaaaagc ccgcagccac agaggctccg gaagcgtcgg cgaccccggc
     2821 agccccggcg gcgcccgtga aggggctggg tctcgcggca ggcgccaagc gtcccggcgc
     2881 caagaagacc gcagccgcac cggccgaaaa gcccgcagcc gcagagaccg aggcaccggc
     2941 gccggcagaa accgcggctc cggccgagcc ggccaagccc gaaccgcccg tcgtgggcct
     3001 cggcatcgcc gcgggcgctc gccgtccggg tgccaagaag gcggccgcca agcctgccgc
     3061 tgcgccggcg cccgccgccg agaagccggc cgagcaggcc gcggagcccg aaaagcccgc
     3121 ggagaagccg gctgagcccg agaagccgga accgcccgtg gtggggctcg gcatcaagcc
     3181 gggcgccaag cggcccggta agcgcaagct tggtgcggcc gcgggcaaca cgcctggcat
     3241 caatctgatc aaggaggaca tgcgtgtcaa ggtgcatatg gagggcaacg tgaacggtca
     3301 cgccttcgtg atcgaggggg agggtaaggg taagccttac gaagggaccc agaccgccaa
     3361 cctcaccgtc aaggagggcg cccctctccc attctcgtac gatatcctca ctaccgcggt
     3421 gcattacggg aaccgcgtgt tcaccaagta ccccgaggat atccctgact acttcaagca
     3481 gagcttcccg gagggatact cctgggagcg gacgatgacc ttcgaagaca agggtatctg
     3541 tacgatccgc agcgacatct cgctggaagg ggactgtttc ttccagaacg tccgtttcaa
     3601 ggggaccaac tttccgccga acggcccggt catgcagaag aagaccctca agtgggagcc
     3661 gagcacggag aagctgcacg ttcgggacgg cctcctggtc ggcaacatca acatggcgct
     3721 gctgctggaa ggcggcggcc actacctatg cgacttcaag accacgtaca aagcgaagaa
     3781 ggtcgtccag ctgcccgacg cgcacttcgt ggaccaccgc atcgaaatcc tggggaacga
     3841 ttcggactac aacaaggtca agttgtacga gcacgctgtg gcacgctata gcccgctccc
     3901 gtcgcaggtc tggttcgaag gcggcggcgg ggagaacctg tatttccagg actacaagga
     3961 cgacgacgac aagtgattta aataacccct tggggcctct aaacgggtct tgaggggttt
     4021 tttgttaact agcgtacgat cgaattcctg cagctcacgg taactgatgc cgtatttgca
     4081 gtaccagcgt acggcccaca gaatgatgtc acgctgaaaa tgccggcctt tgaatgggtt
     4141 catgtgcagc tccatcagca aaaggggatg ataagtttat caccaccgac tatttgcaac
     4201 agtgccgttg atcgtgctat gatcgactga tgtcatcagc ggtggagtgc aatgtcgtgc
     4261 aatacgaatg gcgaaaagcc gagctcatcg gtcagcttct caaccttggg gttacccccg
     4321 gcggtgtgct gctggtccac agctccttcc gtagcgtccg gcccctcgaa gatgggccac
     4381 ttggactgat cgaggccctg cgtgctacgc tgggtccggg agggacgctc gtcatgccct
     4441 cgtggtcagg tctggacgac gagccgttcg atcctgccac gtcgcccgtt acaccggacc
     4501 ttggagttgt ctctgacaca ttctggcgcc tgccaaatgt aaagcgcagc gcccatccat
     4561 ttgcctttgc ggcagcgggg ccacaggcag agcagatcat ctctgatcca ttgcccctgc
     4621 caccttactc gcctgcaagc ccggtcgccc gtgtccatga actcgatggg caggtacttc
     4681 tcctcggcgt gggacacgat gccaacacga cgctgcatct tgccgagttg atggcaaagg
     4741 ttccctatgg ggtgccgaga cactgcacca ttcttcagga tggcaagttg gtacgcgtcg
     4801 attatctcga gaatgaccac tgctgtgagc gctttgcctt ggcggacagg tggctcaagg
     4861 agaagagcct tcagaaggaa ggtccagtcg gtcatgcctt tgctcggttg atccgctccc
     4921 gcgacattgt ggcgacagcc ctgggtcaac tgggccgaga tccgttgatc ttcctgcatc
     4981 cgccagaggc gggatgcgaa gaatgcgatg ccgctcgcca gtcgattggc tgagctcatg
     5041 agcggagaac gagatgacgt tggaggggca aggtcgcgct gattgctggg gcaacacggg
     5101 ggatccacta gttccatgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5161 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5221 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5281 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     5341 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5401 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5461 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5521 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aggggagaaa
     5581 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5641 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5701 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5761 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     5821 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     5881 ccgaacgacc gagcgcaacg cgtgcggccg cggtacccgg ggatcctcta gagtcgacca
     5941 ccaagggcac catctctgct tgggccaccc cgttggccgc agccagctcg ctgagagccg
     6001 tgaacgacag ggcgaacgcc agcccgccga cggcgagggt tccgaccgct gcaactcccg
     6061 gtgcaacctt gtcccggtct attctcttca ctgcaccagc tccaatctgg tgtgaatgcc
     6121 cctcgtctgt tcgcgcaggc ggggggctct attcgtttgt cagcatcgaa agtagccaga
     6181 tcagggatgc gttgcaaccg cgtatgccca ggtcagaaga gtcgcacaag agttgcagac
     6241 ccctggaaag aaaaatggcc agagggcgaa aacaccctct gaccagcgga gcgggcgacg
     6301 ggaatcgaac ccgcgtagct agtttggaag aatgggtgtc tgccgaccac atatgggccg
     6361 gtcaagatag gtttttaccc cctctcggct gcatcctcta agtggaaaga aattgcaggt
     6421 cgtagaagcg cgttgaagcc tgagagttgc acaggagttg caacccggta gccttgttca
     6481 cgacgagagg agacctagtt ggcacgtcgc ggatggggat cgctgaagac tcagcgcagc
     6541 gggaggatcc aagcctcata cgtcaacccg caggacggtg tgaggtacta cgcgctgcag
     6601 acctacgaca acaagatgga cgccgaagcc tggctcgcgg gcgagaagcg gctcatcgag
     6661 atggagacct ggacccctcc acaggaccgg gcgaagaagg cagccgccag cgccatcacg
     6721 ctggaggagt acacccggaa gtggctcgtg gagcgcgacc tcgcagacgg caccagggat
     6781 ctgtacagcg ggcacgcgga gcgccgcatc tacccggtgc taggtgaagt ggcggtcaca
     6841 gagatgacgc cagctctggt gcgtgcgtgg tgggccggga tgggtaggaa gcacccgact
     6901 gcccgccggc atgcctacaa cgtcctccgg gcggtgatga acacagcggt cgaggacaag
     6961 ctgatcgcag agaacccgtg ccggatcgag cagaaggcag ccgatgagcg cgacgtagag
     7021 gcgctgacgc ctgaggagct ggacatcgtc gccgctgaga tcttcgagca ctaccggatc
     7081 gcggcataca tcctggcgtg gacgagcctc cggttcggag agctgatcga gcttcgccgc
     7141 aaggacatcg tggacgacgg catgacgatg aagctccggg tgcgccgtgg cgcttcccgc
     7201 gtggggaaca agatcgtcgt tggcaacgcc aagaccgtcc ggtcgaagcg tcctgtgacg
     7261 gttccgcctc acgtcgcgga gatgatccga gcgcacatga aggaccgtac gaagatgaac
     7321 aagggccccg aggcattcct ggtgaccacg acgcagggca accggctgtc gaagtccgcg
     7381 ttcaccaagt cgctgaagcg tggctacgcc aagatcggtc ggccggaact ccgcatccac
     7441 gacctccgcg ctgtcggcgc tacgttcgcc gctcaggcag gtgcgacgac caaggagctg
     7501 atggcccgtc tcggtcacac gactcctagg atggcgatga agtaccagat ggcgtctgag
     7561 gcccgcgacg aggctatcgc tgaggcgatg tccaagctgg ccaagacctc ctgaaacgca
     7621 aaaagccccc ctcccaagga cactgagtcc taaagagggg ggtttcttgt cagtacgcga
     7681 agaaccacgc ctggccgcga gcgccagcac cgccgctctg tgcggagacc tgggcaccag
     7741 ccccgccgcc gccaggagca ttgccgttcc cgccagaaat ctagacggtg accacaacgc
     7801 gcccgctttg atcggggacg tcgaaatatt ggatcgtcgg caccgtcacg gccgtgggag
     7861 gcggcacgat ccgcgacgtg atgatcggcc gcatccccac ggtgctgcgc agtgagctct
     7921 acgccatccc ggcgttgatc tgtgcgttcg cacgcacagg cccggtgtga gaagggtctc
     7981 tgacgagcgg gagaactccc tatcag
//
