LOCUS       sequence-316079-        5528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0760:Dendra
FEATURES             Location/Qualifiers
     source          1..5528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1472..1495
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1752..2552
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2677..3265
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3166..3185
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgtcggctga cttcggactg gacccgcgcc gcacggaccc
      121 gaaataccac agcgagcacg gcggcttccc cgtgttcgag gccgccgagc ccgggccggg
      181 attcggccgt ttcctcaccg cgatgcgccg cgcgcaggat ctcgcggtct cggccgaccc
      241 ggacgccgcc acgtgggaca aggccgccga cctggtcgag gaactggttg cgctgctcga
      301 tccctacgag gcgggcgagg gcgtcgggcc ggccaatcgc gtcccggaac tgcccggcgc
      361 gggcagcctg ctggcgccac cctggacggt cgagaagttc gaggccgacg aggtgctgct
      421 gaccgtgcag ttcagtcgtt tccacgtggg cggcaactac gccgtccacg gcggcgtgct
      481 gcccatgctt ttcgactcgc tgttcggcat gtgtatccac gcggccggac gccccatcag
      541 ccgcacggcc ttcctgcacg tcgattaccg cgccgtgacg ccgatcgaca ccccgctgca
      601 ggcgcgcggg tggatccgtg aggccgaggg acgcaaggcc tttgtcaacg ccgagctgcg
      661 cgacggtgag ggcaagctcc tcgccgaggc caacggcctg atgatcatgc tgctgcccgg
      721 acagcccaag cttggtgcgg ccgcgggcaa cacgcctggc atcaatctga tcaaggagga
      781 catgcgtgtc aaggtgcata tggagggcaa cgtgaacggt cacgccttcg tgatcgaggg
      841 ggagggtaag ggtaagcctt acgaagggac ccagaccgcc aacctcaccg tcaaggaggg
      901 cgcccctctc ccattctcgt acgatatcct cactaccgcg gtgcattacg ggaaccgcgt
      961 gttcaccaag taccccgagg atatccctga ctacttcaag cagagcttcc cggagggata
     1021 ctcctgggag cggacgatga ccttcgaaga caagggtatc tgtacgatcc gcagcgacat
     1081 ctcgctggaa ggggactgtt tcttccagaa cgtccgtttc aaggggacca actttccgcc
     1141 gaacggcccg gtcatgcaga agaagaccct caagtgggag ccgagcacgg agaagctgca
     1201 cgttcgggac ggcctcctgg tcggcaacat caacatggcg ctgctgctgg aaggcggcgg
     1261 ccactaccta tgcgacttca agaccacgta caaagcgaag aaggtcgtcc agctgcccga
     1321 cgcgcacttc gtggaccacc gcatcgaaat cctggggaac gattcggact acaacaaggt
     1381 caagttgtac gagcacgctg tggcacgcta tagcccgctc ccgtcgcagg tctggttcga
     1441 aggcggcggc ggggagaacc tgtatttcca ggactacaag gacgacgacg acaagtgatt
     1501 taaataaccc cttggggcct ctaaacgggt cttgaggggt tttttgttaa ctagcgtacg
     1561 atcgaattcc tgcagctcac ggtaactgat gccgtatttg cagtaccagc gtacggccca
     1621 cagaatgatg tcacgctgaa aatgccggcc tttgaatggg ttcatgtgca gctccatcag
     1681 caaaagggga tgataagttt atcaccaccg actatttgca acagtgccgt tgatcgtgct
     1741 atgatcgact gatgtcatca gcggtggagt gcaatgtcgt gcaatacgaa tggcgaaaag
     1801 ccgagctcat cggtcagctt ctcaaccttg gggttacccc cggcggtgtg ctgctggtcc
     1861 acagctcctt ccgtagcgtc cggcccctcg aagatgggcc acttggactg atcgaggccc
     1921 tgcgtgctac gctgggtccg ggagggacgc tcgtcatgcc ctcgtggtca ggtctggacg
     1981 acgagccgtt cgatcctgcc acgtcgcccg ttacaccgga ccttggagtt gtctctgaca
     2041 cattctggcg cctgccaaat gtaaagcgca gcgcccatcc atttgccttt gcggcagcgg
     2101 ggccacaggc agagcagatc atctctgatc cattgcccct gccaccttac tcgcctgcaa
     2161 gcccggtcgc ccgtgtccat gaactcgatg ggcaggtact tctcctcggc gtgggacacg
     2221 atgccaacac gacgctgcat cttgccgagt tgatggcaaa ggttccctat ggggtgccga
     2281 gacactgcac cattcttcag gatggcaagt tggtacgcgt cgattatctc gagaatgacc
     2341 actgctgtga gcgctttgcc ttggcggaca ggtggctcaa ggagaagagc cttcagaagg
     2401 aaggtccagt cggtcatgcc tttgctcggt tgatccgctc ccgcgacatt gtggcgacag
     2461 ccctgggtca actgggccga gatccgttga tcttcctgca tccgccagag gcgggatgcg
     2521 aagaatgcga tgccgctcgc cagtcgattg gctgagctca tgagcggaga acgagatgac
     2581 gttggagggg caaggtcgcg ctgattgctg gggcaacacg ggggatccac tagttccatg
     2641 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2701 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2761 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2821 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2881 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2941 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3001 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3061 gcgtgagcta tgagaaagcg ccacgcttcc cgaggggaga aaggcggaca ggtatccggt
     3121 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3181 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3241 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3301 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3361 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcaa
     3421 cgcgtgcggc cgcggtaccc ggggatcctc tagagtcgac caccaagggc accatctctg
     3481 cttgggccac cccgttggcc gcagccagct cgctgagagc cgtgaacgac agggcgaacg
     3541 ccagcccgcc gacggcgagg gttccgaccg ctgcaactcc cggtgcaacc ttgtcccggt
     3601 ctattctctt cactgcacca gctccaatct ggtgtgaatg cccctcgtct gttcgcgcag
     3661 gcggggggct ctattcgttt gtcagcatcg aaagtagcca gatcagggat gcgttgcaac
     3721 cgcgtatgcc caggtcagaa gagtcgcaca agagttgcag acccctggaa agaaaaatgg
     3781 ccagagggcg aaaacaccct ctgaccagcg gagcgggcga cgggaatcga acccgcgtag
     3841 ctagtttgga agaatgggtg tctgccgacc acatatgggc cggtcaagat aggtttttac
     3901 cccctctcgg ctgcatcctc taagtggaaa gaaattgcag gtcgtagaag cgcgttgaag
     3961 cctgagagtt gcacaggagt tgcaacccgg tagccttgtt cacgacgaga ggagacctag
     4021 ttggcacgtc gcggatgggg atcgctgaag actcagcgca gcgggaggat ccaagcctca
     4081 tacgtcaacc cgcaggacgg tgtgaggtac tacgcgctgc agacctacga caacaagatg
     4141 gacgccgaag cctggctcgc gggcgagaag cggctcatcg agatggagac ctggacccct
     4201 ccacaggacc gggcgaagaa ggcagccgcc agcgccatca cgctggagga gtacacccgg
     4261 aagtggctcg tggagcgcga cctcgcagac ggcaccaggg atctgtacag cgggcacgcg
     4321 gagcgccgca tctacccggt gctaggtgaa gtggcggtca cagagatgac gccagctctg
     4381 gtgcgtgcgt ggtgggccgg gatgggtagg aagcacccga ctgcccgccg gcatgcctac
     4441 aacgtcctcc gggcggtgat gaacacagcg gtcgaggaca agctgatcgc agagaacccg
     4501 tgccggatcg agcagaaggc agccgatgag cgcgacgtag aggcgctgac gcctgaggag
     4561 ctggacatcg tcgccgctga gatcttcgag cactaccgga tcgcggcata catcctggcg
     4621 tggacgagcc tccggttcgg agagctgatc gagcttcgcc gcaaggacat cgtggacgac
     4681 ggcatgacga tgaagctccg ggtgcgccgt ggcgcttccc gcgtggggaa caagatcgtc
     4741 gttggcaacg ccaagaccgt ccggtcgaag cgtcctgtga cggttccgcc tcacgtcgcg
     4801 gagatgatcc gagcgcacat gaaggaccgt acgaagatga acaagggccc cgaggcattc
     4861 ctggtgacca cgacgcaggg caaccggctg tcgaagtccg cgttcaccaa gtcgctgaag
     4921 cgtggctacg ccaagatcgg tcggccggaa ctccgcatcc acgacctccg cgctgtcggc
     4981 gctacgttcg ccgctcaggc aggtgcgacg accaaggagc tgatggcccg tctcggtcac
     5041 acgactccta ggatggcgat gaagtaccag atggcgtctg aggcccgcga cgaggctatc
     5101 gctgaggcga tgtccaagct ggccaagacc tcctgaaacg caaaaagccc ccctcccaag
     5161 gacactgagt cctaaagagg ggggtttctt gtcagtacgc gaagaaccac gcctggccgc
     5221 gagcgccagc accgccgctc tgtgcggaga cctgggcacc agccccgccg ccgccaggag
     5281 cattgccgtt cccgccagaa atctagacgg tgaccacaac gcgcccgctt tgatcgggga
     5341 cgtcgaaata ttggatcgtc ggcaccgtca cggccgtggg aggcggcacg atccgcgacg
     5401 tgatgatcgg ccgcatcccc acggtgctgc gcagtgagct ctacgccatc ccggcgttga
     5461 tctgtgcgtt cgcacgcaca ggcccggtgt gagaagggtc tctgacgagc gggagaactc
     5521 cctatcag
//
