LOCUS       sequence-31608-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UEV3
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..21
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             68..85
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttgtttaact ttaagaagga gatataccat gggcagcagc catcatcatc atcatcacag
       61 cagcggcctg gttccgcgtg gtagttcaaa gagctgggca aatcatgaga ataaaacagt
      121 caataaaatt actgtggttg gaggtggaga actcggtatt gcctgcacat tagcaatttc
      181 agcaaagggt attgcagaca ggcttgtcct cttagacctc tcagaaggga ctaaaggagc
      241 cacgatggac cttgaaatct tcaaccttcc taatgtggag atcagcaaag atttgtctgc
      301 ctctgctcat tccaaggtgg tgatcttcac agtcaactct ttgggtagtt ctcagtcgta
      361 ccttgatgtg gtacagagca atgtggatat gttcagagcc cttgtcccag ctctgggaca
      421 ttatagtcaa cacagtgtcc tgctcgttgc atctcaacca gtggaaatca tgacctatgt
      481 aacatggaaa ctgagtacat ttcctgcaaa tcgagtgatc ggaattggat gtaatctgga
      541 ttcacagaga ttacagtata ttattacaaa tgttttgaag gcacagactt caggcaaaga
      601 agtatgggtt attggcgagc aaggagaaga caaagtgctc acatggagtg gccaagaaga
      661 agtagtgagt catacctctc aagtgcagct gtccaacaga gccatggaac tgctaagagt
      721 aaaaggtcaa agatcctggt ctgttggact atcagtagct gacatggttg acagtattgt
      781 aaacaataag aagaaagtgc attctgtatc agctttagca aagggatatt atgatataaa
      841 tagtgaagtg tttttaagtt tgccttgcat ccttggaacc aatggagtat ctgaagttat
      901 caaaaccaca
//
