LOCUS       sequence-316080-        6311 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0154:Dendra
FEATURES             Location/Qualifiers
     source          1..6311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1052..1061
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2255..2278
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2535..3335
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3460..4048
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3949..3968
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tggcgaatcg ccgcgcaaag atcgtctgca cgttgggccc
      121 ggccacggcc acgtcgtcgg tactgaccga actcgtcgac gccggaatgg atgtcgcccg
      181 gctgaacttc agccacagca cccacgccga gcactcggcg ctgtacggca tggtccggga
      241 gatcgccgcc cagcgtggcc gcgtggtcgg cgtcctcgcg gacctgcagg ggcccaagat
      301 ccggctgggg tgtttcgccg acggtcccgt cgtatgggcg acgggcgagc acgtcacgat
      361 caccacggag gactgcccgg gtgaccacga ccgggtctcc acgacctacg cgggcctgtc
      421 acaggacgtg cgcgcgggtg accggctgtt ggtcgacgac gggcgcgtgg atctgcgcgt
      481 ggtggccgtc gacggtcccg acatccggtg cgaggtcgtc gacggcgggc cggtcagcga
      541 ccacaagggg atatcgctgc ccaacatccc ggtgagcgtt ccgccgttgt cggacaagga
      601 catcgaggat ctcaagttcg cgctggagct cggcgcggac atgatcgcga tgtcgttcgt
      661 gcgcgcaccc gaggaggtcg agctcgcgca caagatcatg gacgaggtgg gacgccgggt
      721 cccggtcatc gcgaaactgg agaagcctga ggcggtttcg gacctacccg cgatcgtcga
      781 ggcgttcgac ggtctcatgg tcgcgcgcgg cgatctcggc gtggagatgc ccctggagca
      841 gatcccgctg gtgcagcgcc gcgcgatcgc cctgtgccgt caggcggcca aacccgtgat
      901 cgtggcgacg cagatgctcg attcgatggt gtcggaccgc cgcccgacac gtgccgaggt
      961 gtccgacgtc gccaacgcgg tgttcgacag ggccgatgcg gtgatgctct cggccgagac
     1021 gagtgtgggc gcggatccgg cgcacgccgt cgccaccatg gcacgcatcg tcgtcgccgc
     1081 cgagagcggt tctggccggg gcgagacgcc cgaatcggcg ctcgtggagg ccgtgaacgg
     1141 cacggacgcg gtgttcggtg acgcgatcgc ggtggcggcc tgcgaggtgg gacgccggct
     1201 gggcgccgcg gccctgtgct gcttcacgcg caccggtgac acggcgctgc ggttggcccg
     1261 gcagcggtca ccgctgccgc tgctggcctt cgcgcacgac gacgaggtgc gggggcgcct
     1321 gacgttctcg tggggtgtcg agtcggcggt gctggcaccg acggagctgg ccgaatccat
     1381 gccgggcgag gtgtcgcgtg cgttgctgag catgggcgcg tgccaccccg gcgatgtcgt
     1441 ggtggtcgtc tccggaagcc gaagcggtgt cgcggggtac accgattcgg tccgggtgct
     1501 ccgtgtcgag aagcttggtg cggccgcggg caacacgcct ggcatcaatc tgatcaagga
     1561 ggacatgcgt gtcaaggtgc atatggaggg caacgtgaac ggtcacgcct tcgtgatcga
     1621 gggggagggt aagggtaagc cttacgaagg gacccagacc gccaacctca ccgtcaagga
     1681 gggcgcccct ctcccattct cgtacgatat cctcactacc gcggtgcatt acgggaaccg
     1741 cgtgttcacc aagtaccccg aggatatccc tgactacttc aagcagagct tcccggaggg
     1801 atactcctgg gagcggacga tgaccttcga agacaagggt atctgtacga tccgcagcga
     1861 catctcgctg gaaggggact gtttcttcca gaacgtccgt ttcaagggga ccaactttcc
     1921 gccgaacggc ccggtcatgc agaagaagac cctcaagtgg gagccgagca cggagaagct
     1981 gcacgttcgg gacggcctcc tggtcggcaa catcaacatg gcgctgctgc tggaaggcgg
     2041 cggccactac ctatgcgact tcaagaccac gtacaaagcg aagaaggtcg tccagctgcc
     2101 cgacgcgcac ttcgtggacc accgcatcga aatcctgggg aacgattcgg actacaacaa
     2161 ggtcaagttg tacgagcacg ctgtggcacg ctatagcccg ctcccgtcgc aggtctggtt
     2221 cgaaggcggc ggcggggaga acctgtattt ccaggactac aaggacgacg acgacaagtg
     2281 atttaaataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgt taactagcgt
     2341 acgatcgaat tcctgcagct cacggtaact gatgccgtat ttgcagtacc agcgtacggc
     2401 ccacagaatg atgtcacgct gaaaatgccg gcctttgaat gggttcatgt gcagctccat
     2461 cagcaaaagg ggatgataag tttatcacca ccgactattt gcaacagtgc cgttgatcgt
     2521 gctatgatcg actgatgtca tcagcggtgg agtgcaatgt cgtgcaatac gaatggcgaa
     2581 aagccgagct catcggtcag cttctcaacc ttggggttac ccccggcggt gtgctgctgg
     2641 tccacagctc cttccgtagc gtccggcccc tcgaagatgg gccacttgga ctgatcgagg
     2701 ccctgcgtgc tacgctgggt ccgggaggga cgctcgtcat gccctcgtgg tcaggtctgg
     2761 acgacgagcc gttcgatcct gccacgtcgc ccgttacacc ggaccttgga gttgtctctg
     2821 acacattctg gcgcctgcca aatgtaaagc gcagcgccca tccatttgcc tttgcggcag
     2881 cggggccaca ggcagagcag atcatctctg atccattgcc cctgccacct tactcgcctg
     2941 caagcccggt cgcccgtgtc catgaactcg atgggcaggt acttctcctc ggcgtgggac
     3001 acgatgccaa cacgacgctg catcttgccg agttgatggc aaaggttccc tatggggtgc
     3061 cgagacactg caccattctt caggatggca agttggtacg cgtcgattat ctcgagaatg
     3121 accactgctg tgagcgcttt gccttggcgg acaggtggct caaggagaag agccttcaga
     3181 aggaaggtcc agtcggtcat gcctttgctc ggttgatccg ctcccgcgac attgtggcga
     3241 cagccctggg tcaactgggc cgagatccgt tgatcttcct gcatccgcca gaggcgggat
     3301 gcgaagaatg cgatgccgct cgccagtcga ttggctgagc tcatgagcgg agaacgagat
     3361 gacgttggag gggcaaggtc gcgctgattg ctggggcaac acgggggatc cactagttcc
     3421 atgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3481 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3541 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3601 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3661 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3721 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3781 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3841 acagcgtgag ctatgagaaa gcgccacgct tcccgagggg agaaaggcgg acaggtatcc
     3901 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3961 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4021 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4081 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     4141 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     4201 caacgcgtgc ggccgcggta cccggggatc ctctagagtc gaccaccaag ggcaccatct
     4261 ctgcttgggc caccccgttg gccgcagcca gctcgctgag agccgtgaac gacagggcga
     4321 acgccagccc gccgacggcg agggttccga ccgctgcaac tcccggtgca accttgtccc
     4381 ggtctattct cttcactgca ccagctccaa tctggtgtga atgcccctcg tctgttcgcg
     4441 caggcggggg gctctattcg tttgtcagca tcgaaagtag ccagatcagg gatgcgttgc
     4501 aaccgcgtat gcccaggtca gaagagtcgc acaagagttg cagacccctg gaaagaaaaa
     4561 tggccagagg gcgaaaacac cctctgacca gcggagcggg cgacgggaat cgaacccgcg
     4621 tagctagttt ggaagaatgg gtgtctgccg accacatatg ggccggtcaa gataggtttt
     4681 taccccctct cggctgcatc ctctaagtgg aaagaaattg caggtcgtag aagcgcgttg
     4741 aagcctgaga gttgcacagg agttgcaacc cggtagcctt gttcacgacg agaggagacc
     4801 tagttggcac gtcgcggatg gggatcgctg aagactcagc gcagcgggag gatccaagcc
     4861 tcatacgtca acccgcagga cggtgtgagg tactacgcgc tgcagaccta cgacaacaag
     4921 atggacgccg aagcctggct cgcgggcgag aagcggctca tcgagatgga gacctggacc
     4981 cctccacagg accgggcgaa gaaggcagcc gccagcgcca tcacgctgga ggagtacacc
     5041 cggaagtggc tcgtggagcg cgacctcgca gacggcacca gggatctgta cagcgggcac
     5101 gcggagcgcc gcatctaccc ggtgctaggt gaagtggcgg tcacagagat gacgccagct
     5161 ctggtgcgtg cgtggtgggc cgggatgggt aggaagcacc cgactgcccg ccggcatgcc
     5221 tacaacgtcc tccgggcggt gatgaacaca gcggtcgagg acaagctgat cgcagagaac
     5281 ccgtgccgga tcgagcagaa ggcagccgat gagcgcgacg tagaggcgct gacgcctgag
     5341 gagctggaca tcgtcgccgc tgagatcttc gagcactacc ggatcgcggc atacatcctg
     5401 gcgtggacga gcctccggtt cggagagctg atcgagcttc gccgcaagga catcgtggac
     5461 gacggcatga cgatgaagct ccgggtgcgc cgtggcgctt cccgcgtggg gaacaagatc
     5521 gtcgttggca acgccaagac cgtccggtcg aagcgtcctg tgacggttcc gcctcacgtc
     5581 gcggagatga tccgagcgca catgaaggac cgtacgaaga tgaacaaggg ccccgaggca
     5641 ttcctggtga ccacgacgca gggcaaccgg ctgtcgaagt ccgcgttcac caagtcgctg
     5701 aagcgtggct acgccaagat cggtcggccg gaactccgca tccacgacct ccgcgctgtc
     5761 ggcgctacgt tcgccgctca ggcaggtgcg acgaccaagg agctgatggc ccgtctcggt
     5821 cacacgactc ctaggatggc gatgaagtac cagatggcgt ctgaggcccg cgacgaggct
     5881 atcgctgagg cgatgtccaa gctggccaag acctcctgaa acgcaaaaag cccccctccc
     5941 aaggacactg agtcctaaag aggggggttt cttgtcagta cgcgaagaac cacgcctggc
     6001 cgcgagcgcc agcaccgccg ctctgtgcgg agacctgggc accagccccg ccgccgccag
     6061 gagcattgcc gttcccgcca gaaatctaga cggtgaccac aacgcgcccg ctttgatcgg
     6121 ggacgtcgaa atattggatc gtcggcaccg tcacggccgt gggaggcggc acgatccgcg
     6181 acgtgatgat cggccgcatc cccacggtgc tgcgcagtga gctctacgcc atcccggcgt
     6241 tgatctgtgc gttcgcacgc acaggcccgg tgtgagaagg gtctctgacg agcgggagaa
     6301 ctccctatca g
//
