LOCUS       sequence-316081-        8855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 8855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0617:Dendra
FEATURES             Location/Qualifiers
     source          1..8855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4799..4822
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5079..5879
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      6004..6592
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6493..6512
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgagccggct catcttcgag caccagcgtc ggctgacgcc
      121 gcccaccacc cgcaagggca ccatcacgat cgaaccgcca ccgcagttgc cgcgcgtggt
      181 gccaccgtcg ctgctgcgcc gggtgctgcc gtttctgatc gtgatcctga tcgtgggcat
      241 gatcgtggcg ctgttcgcca ccggtatgcg gctgatctcg ccgaccatgc tgttcttccc
      301 gttcgtcctg ctgctggccg ccaccgcgct ctaccgcggc ggcgacaaca agatgcgcac
      361 cgaggaggtc gacgccgaac gcgccgacta cctgcggtac ctgtcggtgg tgcgcgacaa
      421 cgtgcgcgcg cacgccgccg agcagcgcgc cgcgctcgag tggtcacacc ccgaacccga
      481 agtgctggcg acgattcccg gcacgcgcag gcagtgggag cgcgacccgc gcgaccgtga
      541 cttcctggtg ctgcgggccg gcaggcacga cgtgccgctg gacgccgcgc tgaaggtcaa
      601 ggacaccgcc gacgagatcg acctcgagcc cgtggcgcac agcgcgctgc gtggcctgct
      661 cgacgtgcag cgcaccgtgc gcgacgcacc cacgggcctc gacgtcgcca agctggcccg
      721 catcaccgtg atcggcgagg ccgacgaggc tcgcgccgcg atccgggcct ggatcgcgca
      781 ggccgtcacg tggcacgacc ccaccatgct cggggtggcg ctcgccgcgc ccgatctgga
      841 gtccggtgac tggtcgtggc tgaaatggct tccgcacgta gatgtcccga acgaggccga
      901 cggtgtcggg ccggcgcggt acctgaccac gtccacagcc gaactgcgtg agcggctggc
      961 tccggcgctg gccgaccggc cgctgttccc ggccgagtcc ggcgcggcgc tcaagcatct
     1021 gctggtggtg ctcgacgacc ccgatgccga tcccgacgac atcgcccgca agcccgggct
     1081 gaccggtgtc accgtcatcc accgcaccac ggaactgccc aaccgcgagc agtacccgga
     1141 tccagagcgg cccatcctgc gcgtcgccga cggccggatc gagcgctggc aggtgggtgg
     1201 ctggcagccc tgtgtcgatg tggccgacgc gatgtcggcg gccgaggccg cgcacatcgc
     1261 gcggcggctg tcgcggtggg actccaaccc gggatacatc cgctccacct cgaccggcag
     1321 tgcgacgttc acgacgctgc tcggcatccc cgatgcctcg gcgctcgacg tggcgagcct
     1381 gtgggcgccc cggccgcgcg acgaggaact gcgcgtgccg atcggcgtca cctcgaccgg
     1441 tgagccgctg tacttcgacc tcaaggacga ggccgagggc ggtatgggcc cgcacgggct
     1501 gatgatcggt atgacgggtt cgggcaagtc gcagaccctg atgtcgatcc tgttgtcgct
     1561 gttgaccacc catcccgccg atcgcctcat cgtgatctac gcggacttca agggtgaggc
     1621 cggagccgac atcttccgcc acttcccgca ggtcgtggcg gtcatctcca acatggccga
     1681 gaagcggtcg ctggccgaca ggttcgccga cacgctgcgc ggtgaggtgg cccggcgcga
     1741 gcagatcctc aaggaggccg ggcgccgggt gcagggcagc gcgttcaact cggtcgccga
     1801 gtacgagagc gcgatcgccg caggccacga cctgccgccc atgcccacac tgttcgtggt
     1861 cgccgacgag ttcacgctca tgctcgccga acaccccgag tacgccgacc tgttcgatta
     1921 cgttgcgcgc aagggccgct cgttccggat ccacctgctg ttcgcgtcgc agacactgga
     1981 cgtgggccgt atcaaggaca tcgacaagaa cacctcgtac cgcatcggtc tcaaggtggc
     2041 cagcccgagc atctcgcggc agatcatcgg tgtcgaggac gcctaccaca tcgagtccgg
     2101 ccgcgaacac aaaggcgagg gcttcctggt acccgcgccc ggtgcggtgc cgatcaagtt
     2161 ccgcagcacc tacgtcgacg gcatctacga tccaccacgc gccgagaagt cgattgtggt
     2221 gcatgcactt ccgcaaccgc aggtgttcac ggcgggacgc gtcgaacccg aacccgacac
     2281 cgtgatcgcc acgggcgacg tcgaggtgca caccgcgccg ccgcgcaagt tgatcgccac
     2341 catcggcgac caactggcgg cctacggacc caaggccccg cagctgtggc tgccgccgct
     2401 ggacgagccg atcgcactgg ccgacgtgct ggccggtgcc gacgtcgagc cgggtcagct
     2461 gcgctggccg ctgggtgaga tcgacaagcc gttcgagatg cgacgcgacg tgctggtgta
     2521 cgacgcgcac accgcggccg ccaacgtgct catccacggc ggcccgcggt caggcaagtc
     2581 cacagcactg caggcgttcg tcctgagcgc cgcggcgctg cactcgccgc gcgcgatcac
     2641 cttctactgc ctcgattacg gcggcgggaa gctggccgac ctcgcggacc tggcgcacgt
     2701 cggcagcgtc gcgaccccgc tggagcccga gcgtatccgc cgcacgttcg gcgagctgga
     2761 gcagttgctg agggcgcgtc agcgccaggg cgcggtcaac cgcacgggct cgtacacgga
     2821 cggctacggc gaagtgttcc tggtgatcga caacctgtac gcgttcagcc gcgacaacac
     2881 cgacaccttc aacacccgca acccgttgct ggccaaggtc accgagctgg ccaacagcgg
     2941 cctggcctac ggcatccacg tcgtcatcac cacgccgaac tggctggagg tgccgctggc
     3001 gatgcgcgac ggcctcggtc tgcgtctcga gctcaaactg cacgactcgc acgacagcat
     3061 cgtgcgcgtg gccggtgctc tgcgcaggcc tgcagattcg gtgccggccg atcagccggg
     3121 ccgcggcctg accatggccg ccgaacactt cctgttcgcc gaaccggcgc tgagcgacat
     3181 cgcggtcatc aacgcgcggt acccgggcgt cagcgcaccg cccgtgcgcc tgctgcccac
     3241 agacctgtcg cccgacgcac tggctccgct gtacccggct ccggaaaccg tggtcatcgg
     3301 tcagcgcgaa gaggatctgg cgccggtggc cgtcgacttc gcgaaccacc cgctgctcat
     3361 ggtgttcggc gactcgaagt cgggcaagac gacgctgctg cgccacatca tccgcaccgt
     3421 gcgggagaac tcgacaccgg accaggtcgc gttcacggtg atcgaccgca ggctgcatct
     3481 ggtcgacgaa ccgcttttcc cggacaacga gtacaccgcg aacatcgacc gtgtgctacc
     3541 cgcgatgctt gggctgtcgg ctctcatcga gaagcggcgc ccgcctgcgg ggttgtcagc
     3601 tcaagagctc agccggtgga cctacacggg ccacacgcac tacctgatcg tcgacgacgt
     3661 ggaccagatt ccggacacgc ccgcggtgag cggaccgttc gtgggtcagc gtccgtggac
     3721 gaacatcgtc ggattgctgg ccgaggcagc cgatctcggc ctgcgcgtga tcgtcaccgc
     3781 acgtgccacc ggctcggccc acgcggtgat gacggcaccg ctgctgcgcc gcctcaacga
     3841 tctgcaggcg accacgctga tgctgtcggg caatcccacc gacagcggca agatccgcgg
     3901 ccaccggttc gcccggttcc cggccggccg tggcctgctg ctgaccgaca ccgacacccc
     3961 cgaccacatc cagttggtca acccgctcgg cgacgcggcc ctgagcggaa acatcgggaa
     4021 caacgggaat cacaaccgag gaggggaata ccgaaagctt ggtgcggccg cgggcaacac
     4081 gcctggcatc aatctgatca aggaggacat gcgtgtcaag gtgcatatgg agggcaacgt
     4141 gaacggtcac gccttcgtga tcgaggggga gggtaagggt aagccttacg aagggaccca
     4201 gaccgccaac ctcaccgtca aggagggcgc ccctctccca ttctcgtacg atatcctcac
     4261 taccgcggtg cattacggga accgcgtgtt caccaagtac cccgaggata tccctgacta
     4321 cttcaagcag agcttcccgg agggatactc ctgggagcgg acgatgacct tcgaagacaa
     4381 gggtatctgt acgatccgca gcgacatctc gctggaaggg gactgtttct tccagaacgt
     4441 ccgtttcaag gggaccaact ttccgccgaa cggcccggtc atgcagaaga agaccctcaa
     4501 gtgggagccg agcacggaga agctgcacgt tcgggacggc ctcctggtcg gcaacatcaa
     4561 catggcgctg ctgctggaag gcggcggcca ctacctatgc gacttcaaga ccacgtacaa
     4621 agcgaagaag gtcgtccagc tgcccgacgc gcacttcgtg gaccaccgca tcgaaatcct
     4681 ggggaacgat tcggactaca acaaggtcaa gttgtacgag cacgctgtgg cacgctatag
     4741 cccgctcccg tcgcaggtct ggttcgaagg cggcggcggg gagaacctgt atttccagga
     4801 ctacaaggac gacgacgaca agtgatttaa ataacccctt ggggcctcta aacgggtctt
     4861 gaggggtttt ttgttaacta gcgtacgatc gaattcctgc agctcacggt aactgatgcc
     4921 gtatttgcag taccagcgta cggcccacag aatgatgtca cgctgaaaat gccggccttt
     4981 gaatgggttc atgtgcagct ccatcagcaa aaggggatga taagtttatc accaccgact
     5041 atttgcaaca gtgccgttga tcgtgctatg atcgactgat gtcatcagcg gtggagtgca
     5101 atgtcgtgca atacgaatgg cgaaaagccg agctcatcgg tcagcttctc aaccttgggg
     5161 ttacccccgg cggtgtgctg ctggtccaca gctccttccg tagcgtccgg cccctcgaag
     5221 atgggccact tggactgatc gaggccctgc gtgctacgct gggtccggga gggacgctcg
     5281 tcatgccctc gtggtcaggt ctggacgacg agccgttcga tcctgccacg tcgcccgtta
     5341 caccggacct tggagttgtc tctgacacat tctggcgcct gccaaatgta aagcgcagcg
     5401 cccatccatt tgcctttgcg gcagcggggc cacaggcaga gcagatcatc tctgatccat
     5461 tgcccctgcc accttactcg cctgcaagcc cggtcgcccg tgtccatgaa ctcgatgggc
     5521 aggtacttct cctcggcgtg ggacacgatg ccaacacgac gctgcatctt gccgagttga
     5581 tggcaaaggt tccctatggg gtgccgagac actgcaccat tcttcaggat ggcaagttgg
     5641 tacgcgtcga ttatctcgag aatgaccact gctgtgagcg ctttgccttg gcggacaggt
     5701 ggctcaagga gaagagcctt cagaaggaag gtccagtcgg tcatgccttt gctcggttga
     5761 tccgctcccg cgacattgtg gcgacagccc tgggtcaact gggccgagat ccgttgatct
     5821 tcctgcatcc gccagaggcg ggatgcgaag aatgcgatgc cgctcgccag tcgattggct
     5881 gagctcatga gcggagaacg agatgacgtt ggaggggcaa ggtcgcgctg attgctgggg
     5941 caacacgggg gatccactag ttccatgagc gtcagacccc gtagaaaaga tcaaaggatc
     6001 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6061 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6121 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     6181 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6241 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6301 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6361 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6421 ggggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6481 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6541 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6601 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     6661 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     6721 tcgccgcagc cgaacgaccg agcgcaacgc gtgcggccgc ggtacccggg gatcctctag
     6781 agtcgaccac caagggcacc atctctgctt gggccacccc gttggccgca gccagctcgc
     6841 tgagagccgt gaacgacagg gcgaacgcca gcccgccgac ggcgagggtt ccgaccgctg
     6901 caactcccgg tgcaaccttg tcccggtcta ttctcttcac tgcaccagct ccaatctggt
     6961 gtgaatgccc ctcgtctgtt cgcgcaggcg gggggctcta ttcgtttgtc agcatcgaaa
     7021 gtagccagat cagggatgcg ttgcaaccgc gtatgcccag gtcagaagag tcgcacaaga
     7081 gttgcagacc cctggaaaga aaaatggcca gagggcgaaa acaccctctg accagcggag
     7141 cgggcgacgg gaatcgaacc cgcgtagcta gtttggaaga atgggtgtct gccgaccaca
     7201 tatgggccgg tcaagatagg tttttacccc ctctcggctg catcctctaa gtggaaagaa
     7261 attgcaggtc gtagaagcgc gttgaagcct gagagttgca caggagttgc aacccggtag
     7321 ccttgttcac gacgagagga gacctagttg gcacgtcgcg gatggggatc gctgaagact
     7381 cagcgcagcg ggaggatcca agcctcatac gtcaacccgc aggacggtgt gaggtactac
     7441 gcgctgcaga cctacgacaa caagatggac gccgaagcct ggctcgcggg cgagaagcgg
     7501 ctcatcgaga tggagacctg gacccctcca caggaccggg cgaagaaggc agccgccagc
     7561 gccatcacgc tggaggagta cacccggaag tggctcgtgg agcgcgacct cgcagacggc
     7621 accagggatc tgtacagcgg gcacgcggag cgccgcatct acccggtgct aggtgaagtg
     7681 gcggtcacag agatgacgcc agctctggtg cgtgcgtggt gggccgggat gggtaggaag
     7741 cacccgactg cccgccggca tgcctacaac gtcctccggg cggtgatgaa cacagcggtc
     7801 gaggacaagc tgatcgcaga gaacccgtgc cggatcgagc agaaggcagc cgatgagcgc
     7861 gacgtagagg cgctgacgcc tgaggagctg gacatcgtcg ccgctgagat cttcgagcac
     7921 taccggatcg cggcatacat cctggcgtgg acgagcctcc ggttcggaga gctgatcgag
     7981 cttcgccgca aggacatcgt ggacgacggc atgacgatga agctccgggt gcgccgtggc
     8041 gcttcccgcg tggggaacaa gatcgtcgtt ggcaacgcca agaccgtccg gtcgaagcgt
     8101 cctgtgacgg ttccgcctca cgtcgcggag atgatccgag cgcacatgaa ggaccgtacg
     8161 aagatgaaca agggccccga ggcattcctg gtgaccacga cgcagggcaa ccggctgtcg
     8221 aagtccgcgt tcaccaagtc gctgaagcgt ggctacgcca agatcggtcg gccggaactc
     8281 cgcatccacg acctccgcgc tgtcggcgct acgttcgccg ctcaggcagg tgcgacgacc
     8341 aaggagctga tggcccgtct cggtcacacg actcctagga tggcgatgaa gtaccagatg
     8401 gcgtctgagg cccgcgacga ggctatcgct gaggcgatgt ccaagctggc caagacctcc
     8461 tgaaacgcaa aaagcccccc tcccaaggac actgagtcct aaagaggggg gtttcttgtc
     8521 agtacgcgaa gaaccacgcc tggccgcgag cgccagcacc gccgctctgt gcggagacct
     8581 gggcaccagc cccgccgccg ccaggagcat tgccgttccc gccagaaatc tagacggtga
     8641 ccacaacgcg cccgctttga tcggggacgt cgaaatattg gatcgtcggc accgtcacgg
     8701 ccgtgggagg cggcacgatc cgcgacgtga tgatcggccg catccccacg gtgctgcgca
     8761 gtgagctcta cgccatcccg gcgttgatct gtgcgttcgc acgcacaggc ccggtgtgag
     8821 aagggtctct gacgagcggg agaactccct atcag
//
