LOCUS       sequence-316082-        6063 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  split YFP. Plant expression of CBL6-YFPc-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6063)
  TITLE     Robatzek Lab plasmids
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAM-35s-CBL6-YFPc-1
FEATURES             Location/Qualifiers
     source          1..6063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    298..322
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        1044..1135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1136..1204,1205..1996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1354..1373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2167..2755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2656..2675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    3067..3091
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(3185..3437)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3457..4008)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(4050..4229)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4698..5042
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     4986..5006
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     5044..5060
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5045..5061
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5077..5101
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5783..5807)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(5818..6063,1..6)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     6006..6027
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tacaagtaat ctagagtccg caaaaatcac cagtctctct ctacaaatct atctctctct
       61 atttttctcc agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg
      121 gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata
      181 cttctatcaa taaaatttct aattcctaaa accaaaatcc agtgaccggg cggccgccac
      241 cgcggtggag ggggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga
      301 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt
      361 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgtacatc accgacgagc
      421 aaggcaagac cgagcgcctt tccgacgctc accgggctgg ttgccctcgc cgctgggctg
      481 gcggccgtct atggccctgc aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac
      541 cggccgccgg cgttgtggat acctcgcgga aaacttggcc ctcactgaca gatgaggggc
      601 ggacgttgac acttgagggg cgcgactact gacagatgag gggcgcgatc cttgacactt
      661 gaggggcaga gtgctgacag atgaggggcg cacctattga catttgaggg gctgtccaca
      721 ggcagaaaat ccagcatttg caagggtttc cgcccgtttt tcggccaccg ctaacctgtc
      781 ttttaacctg cttttaaacc aatatttata aaccttgttt ttaaccaggg ctgcgccctg
      841 tgcgcgtgac cgcgcacgcc gaaggggggt gccccccctt ctcgaaccct cccggcccgc
      901 taacgcgggc ctcccatccc cccaggggct gcgcccctcg gccgcgaacg gcctcacccc
      961 aaaaatggca gcgctggcag tccttgccat tgccgggatc ggggcagtaa cgggatgggc
     1021 gatcagcccg aaagctaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     1081 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     1141 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     1201 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     1261 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     1321 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     1381 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     1441 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     1501 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     1561 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     1621 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     1681 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     1741 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     1801 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     1861 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     1921 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     1981 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     2041 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     2101 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2161 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2221 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2281 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     2341 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2401 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2461 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2521 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2581 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2641 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2701 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2761 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatggactc
     2821 tagctagagg atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc
     2881 gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg
     2941 taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga
     3001 tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct
     3061 ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac ttaataacac
     3121 attgcggacg tttttaatga tcgaatacta acgtctctac cagatatcag cttgcatgcc
     3181 ggtcgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat
     3241 attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat
     3301 ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga
     3361 aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc
     3421 caaatgtttg aacgatctgc ttgactctag gggtcatcag atttcggtga cgggcaggac
     3481 cggacggggc ggcaccggca ggctgaagtc cagctgccag aaacccacgt catgccagtt
     3541 cccgtgcttg aagccggccg cccgcagcat gccgcggggg gcatatccga gcgcctcgtg
     3601 catgcgcacg ctcgggtcgt tgggcagccc gatgacagcg accacgctct tgaagccctg
     3661 tgcctccagg gacttcagca ggtgggtgta gagcgtggag cccagtcccg tccgctggtg
     3721 gcggggggag acgtacacgg tcgactcggc cgtccagtcg taggcgttgc gtgccttcca
     3781 gggacccgcg taggcgatgc cggcgacctc gccgtccacc tcggcgacga gccagggata
     3841 gcgctcccgc agacggacga ggtcgtccgt ccactcctgc ggttcctgcg gctcggtacg
     3901 gaagttgacc gtgcttgtct cgatgtagtg gttgacgatg gtgcagaccg ccggcatgtc
     3961 cgcctcggtg gcacggcgga tgtcggccgg gcgtcgttct gggctcatgg tagatcccct
     4021 cgatcgagtt gagagtgaat atgagactct aattggatac cgaggggaat ttatggaacg
     4081 tcagtggagc atttttgaca agaaatattt gctagctgat agtgacctta ggcgactttt
     4141 gaacgcgcaa taatggtttc tgacgtatgt gcttagctca ttaaactcca gaaacccgcg
     4201 gctcagtggc tccttcaacg ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc
     4261 gcgtcatcgg cgggggtcat aacgtgactc ccttaattct ccgctcatga tcagattgtc
     4321 gtttcccgcc ttcggtttgg gcgcgcccgg ttcagaagac cagagggcta ttgagacttt
     4381 tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt
     4441 catcgaaagg acagtagaaa aggaagatgg cttctacaaa tgccatcatt gcgataaagg
     4501 aaaggctatc gttcaagatg cctctaccga cagtggtccc aaagatggac ccccacccac
     4561 gaggaacatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
     4621 tgatacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca
     4681 gaagaccaga gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga
     4741 ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga agatggcttc
     4801 tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc taccgacagt
     4861 ggtcccaaag atggaccccc acccacgagg aacatcgtgg aaaaagaaga cgttccaacc
     4921 acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa
     4981 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
     5041 acctcgaggt cgacggtatc gataagcttc ccatcaacaa gtttgtacaa aaaagcaggc
     5101 ttgatgatga tgcaatgttt agatggatta aagcacttag ccttaattct gttgacttgt
     5161 tgtgatgctg atcctcccaa ggttcgtcag aaccctaagg atgtggctag aggcactgtg
     5221 tttacagtga atgagattga agctctttac gaactgttca agagcattag caaaaacggg
     5281 cttatcgaca aggaacaatt tcaattggta ctattcaaaa tgaacacaac gagaagcctt
     5341 tttgctgata gggtgtttga cttgtttgat accaagaata ctgggattct agattttgaa
     5401 gcctttgctc gttcactttc ggtctttcac cctaatgcta aatttgagga taagattgag
     5461 ttctctttca agctgtatga tctgaatcag caaggttata taaagagaca ggaggtgaag
     5521 caaatggttg tgcggactct agctgaatct ggcatgaacc tttctgatca tgtgattgag
     5581 agcatcatag acaagacatt tgaagaagct gatactaaac tggacgggaa gattgataag
     5641 gaagagtgga ggagtcttgt gttgcgccac ccctctctgt tacagaacat gagccttcaa
     5701 catctcaaag acgtaacaaa gacgtttcca aattttgtat ttcacactat agtgacagac
     5761 actcctagtg agctggatgg atacccagct ttcttgtaca aagtggttga tgggatggac
     5821 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc
     5881 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     5941 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
     6001 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     6061 ctg
//
