LOCUS       sequence-316083-        6905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0005:Dendra
FEATURES             Location/Qualifiers
     source          1..6905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2849..2872
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3129..3929
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      4054..4642
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4543..4562
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tggctgccca gaagaacaat gcgcccaagg agtatggcgc
      121 cgattccatc accattctcg agggtttgga agcggtccgc aaacgtcctg gcatgtacat
      181 cggctcgacc ggcgaacgcg gtcttcacca cctgatctgg gaggtcgtgg acaacgcggt
      241 cgacgaggcg atggccgggt tcgccacgcg cgtcgacgtc aagatccatg ccgacggcag
      301 cgtcgaggtc cgcgacgacg gccgtggcat cccggtcgag atgcacgcga cgggcatgcc
      361 caccatcgat gtcgtcatga cccagctgca cgcgggtggc aagttcgacg gcgagacgta
      421 cgcggtgtcc ggtggtctgc acggcgtggg cgtctcggtc gtgaacgcgc tgtcgacgcg
      481 gctggaggcc acggtcctgc gtgacgggta cgagtggttc cagtactacg accgctcggt
      541 gcccggcaag ctcaagcagg gcggtgagac caaggagacc ggcaccacca tccggttctg
      601 ggcagatccg gagatcttcg aaacgaccga ctacaacttc gagacggtcg cgcgccggct
      661 tcaggagatg gcgttcctca acaagggcct gaccatcgag ctgaccgacg agcgcgtgac
      721 tgccgaagag gtggtcgacg acgtcgtgaa ggacaccgcc gaagcgccca agacggccga
      781 cgagaaagcc gccgaggcca ccgggcccag caaggtcaag caccgcgtct tccattaccc
      841 gggtggtctg gtcgactacg tcaagcacat caaccgcacg aaaaccccca tccagcagag
      901 catcatcgac ttcgacggca agggtcccgg ccatgaggtc gagatcgcga tgcagtggaa
      961 cgcggggtat tcggagtcgg tgcacacctt cgccaacacc atcaacacgc atgagggtgg
     1021 tacgcacgag gagggtttcc gtgcggcgct gaccagtgtg gtgaaccggt atgccaagga
     1081 caagaagctg ctcaaggaca aggatcccaa cctcaccggt gatgacatcc gtgagggttt
     1141 ggcggcggtg atctcggtga aggtcgccga accgcagttc gagggccaga ccaagaccaa
     1201 gctgggcaac accgaggtca agtcgttcgt ccagaagatc tgcaacgagc agctgcagca
     1261 ctggttcgag gccaaccctg ctgaagcgaa aaccgttgtc aacaaagcgg tctcgtcggc
     1321 gcaggcgcgg atcgcggcgc gtaaggcgcg tgagttggtg cggcgtaaga gcgcgaccga
     1381 catcggtggg ttgccgggca agctggccga ctgccgttcg acggatccga gcaagtcgga
     1441 gctgtatgtg gtggagggtg actccgcggg tggttcggcc aagagtggtc gggattcgat
     1501 gttccaggcg atcctgccgt tgcgcggcaa gatcatcaac gtcgagaagg cccgcatcga
     1561 ccgcgtgctg aagaacaccg aggtgcagtc gatcatcacc gcgctgggca ccggtatcca
     1621 tgacgagttc gacatctcca agctgcggta tcacaagatc gtgttgatgg ccgatgcgga
     1681 tgtggacggt cagcacatct cgacgttgtt gttgacgttg ttgttccggt tcatgaagcc
     1741 gctggtggag aacggccaca tcttcttggc gcagccgccg ttgtacaagc tcaagtggca
     1801 gcgcagtgag ccggagttcg cctattccga tcgtgagcgc gacgggctgt tggaggccgg
     1861 gcgcgcggcg ggcaagaaga tcaacgtcga cgacggtatc cagcgctaca agggtctcgg
     1921 tgagatggac gccaaggagt tgtgggagac cacgatggat ccgtcggtgc gggtgctgcg
     1981 acaggtcacc ctcgacgacg ccgccgcggc cgacgaactg ttctccatcc tcatgggcga
     2041 agacgtcgag gcgcgccgta gcttcatcac gcgcaacgcc aaagacgttc gcttcctgga
     2101 tgttaagctt ggtgcggccg cgggcaacac gcctggcatc aatctgatca aggaggacat
     2161 gcgtgtcaag gtgcatatgg agggcaacgt gaacggtcac gccttcgtga tcgaggggga
     2221 gggtaagggt aagccttacg aagggaccca gaccgccaac ctcaccgtca aggagggcgc
     2281 ccctctccca ttctcgtacg atatcctcac taccgcggtg cattacggga accgcgtgtt
     2341 caccaagtac cccgaggata tccctgacta cttcaagcag agcttcccgg agggatactc
     2401 ctgggagcgg acgatgacct tcgaagacaa gggtatctgt acgatccgca gcgacatctc
     2461 gctggaaggg gactgtttct tccagaacgt ccgtttcaag gggaccaact ttccgccgaa
     2521 cggcccggtc atgcagaaga agaccctcaa gtgggagccg agcacggaga agctgcacgt
     2581 tcgggacggc ctcctggtcg gcaacatcaa catggcgctg ctgctggaag gcggcggcca
     2641 ctacctatgc gacttcaaga ccacgtacaa agcgaagaag gtcgtccagc tgcccgacgc
     2701 gcacttcgtg gaccaccgca tcgaaatcct ggggaacgat tcggactaca acaaggtcaa
     2761 gttgtacgag cacgctgtgg cacgctatag cccgctcccg tcgcaggtct ggttcgaagg
     2821 cggcggcggg gagaacctgt atttccagga ctacaaggac gacgacgaca agtgatttaa
     2881 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgttaacta gcgtacgatc
     2941 gaattcctgc agctcacggt aactgatgcc gtatttgcag taccagcgta cggcccacag
     3001 aatgatgtca cgctgaaaat gccggccttt gaatgggttc atgtgcagct ccatcagcaa
     3061 aaggggatga taagtttatc accaccgact atttgcaaca gtgccgttga tcgtgctatg
     3121 atcgactgat gtcatcagcg gtggagtgca atgtcgtgca atacgaatgg cgaaaagccg
     3181 agctcatcgg tcagcttctc aaccttgggg ttacccccgg cggtgtgctg ctggtccaca
     3241 gctccttccg tagcgtccgg cccctcgaag atgggccact tggactgatc gaggccctgc
     3301 gtgctacgct gggtccggga gggacgctcg tcatgccctc gtggtcaggt ctggacgacg
     3361 agccgttcga tcctgccacg tcgcccgtta caccggacct tggagttgtc tctgacacat
     3421 tctggcgcct gccaaatgta aagcgcagcg cccatccatt tgcctttgcg gcagcggggc
     3481 cacaggcaga gcagatcatc tctgatccat tgcccctgcc accttactcg cctgcaagcc
     3541 cggtcgcccg tgtccatgaa ctcgatgggc aggtacttct cctcggcgtg ggacacgatg
     3601 ccaacacgac gctgcatctt gccgagttga tggcaaaggt tccctatggg gtgccgagac
     3661 actgcaccat tcttcaggat ggcaagttgg tacgcgtcga ttatctcgag aatgaccact
     3721 gctgtgagcg ctttgccttg gcggacaggt ggctcaagga gaagagcctt cagaaggaag
     3781 gtccagtcgg tcatgccttt gctcggttga tccgctcccg cgacattgtg gcgacagccc
     3841 tgggtcaact gggccgagat ccgttgatct tcctgcatcc gccagaggcg ggatgcgaag
     3901 aatgcgatgc cgctcgccag tcgattggct gagctcatga gcggagaacg agatgacgtt
     3961 ggaggggcaa ggtcgcgctg attgctgggg caacacgggg gatccactag ttccatgagc
     4021 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4081 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4141 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4201 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4261 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4321 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4381 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4441 tgagctatga gaaagcgcca cgcttcccga ggggagaaag gcggacaggt atccggtaag
     4501 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4561 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4621 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4681 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     4741 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcaacgc
     4801 gtgcggccgc ggtacccggg gatcctctag agtcgaccac caagggcacc atctctgctt
     4861 gggccacccc gttggccgca gccagctcgc tgagagccgt gaacgacagg gcgaacgcca
     4921 gcccgccgac ggcgagggtt ccgaccgctg caactcccgg tgcaaccttg tcccggtcta
     4981 ttctcttcac tgcaccagct ccaatctggt gtgaatgccc ctcgtctgtt cgcgcaggcg
     5041 gggggctcta ttcgtttgtc agcatcgaaa gtagccagat cagggatgcg ttgcaaccgc
     5101 gtatgcccag gtcagaagag tcgcacaaga gttgcagacc cctggaaaga aaaatggcca
     5161 gagggcgaaa acaccctctg accagcggag cgggcgacgg gaatcgaacc cgcgtagcta
     5221 gtttggaaga atgggtgtct gccgaccaca tatgggccgg tcaagatagg tttttacccc
     5281 ctctcggctg catcctctaa gtggaaagaa attgcaggtc gtagaagcgc gttgaagcct
     5341 gagagttgca caggagttgc aacccggtag ccttgttcac gacgagagga gacctagttg
     5401 gcacgtcgcg gatggggatc gctgaagact cagcgcagcg ggaggatcca agcctcatac
     5461 gtcaacccgc aggacggtgt gaggtactac gcgctgcaga cctacgacaa caagatggac
     5521 gccgaagcct ggctcgcggg cgagaagcgg ctcatcgaga tggagacctg gacccctcca
     5581 caggaccggg cgaagaaggc agccgccagc gccatcacgc tggaggagta cacccggaag
     5641 tggctcgtgg agcgcgacct cgcagacggc accagggatc tgtacagcgg gcacgcggag
     5701 cgccgcatct acccggtgct aggtgaagtg gcggtcacag agatgacgcc agctctggtg
     5761 cgtgcgtggt gggccgggat gggtaggaag cacccgactg cccgccggca tgcctacaac
     5821 gtcctccggg cggtgatgaa cacagcggtc gaggacaagc tgatcgcaga gaacccgtgc
     5881 cggatcgagc agaaggcagc cgatgagcgc gacgtagagg cgctgacgcc tgaggagctg
     5941 gacatcgtcg ccgctgagat cttcgagcac taccggatcg cggcatacat cctggcgtgg
     6001 acgagcctcc ggttcggaga gctgatcgag cttcgccgca aggacatcgt ggacgacggc
     6061 atgacgatga agctccgggt gcgccgtggc gcttcccgcg tggggaacaa gatcgtcgtt
     6121 ggcaacgcca agaccgtccg gtcgaagcgt cctgtgacgg ttccgcctca cgtcgcggag
     6181 atgatccgag cgcacatgaa ggaccgtacg aagatgaaca agggccccga ggcattcctg
     6241 gtgaccacga cgcagggcaa ccggctgtcg aagtccgcgt tcaccaagtc gctgaagcgt
     6301 ggctacgcca agatcggtcg gccggaactc cgcatccacg acctccgcgc tgtcggcgct
     6361 acgttcgccg ctcaggcagg tgcgacgacc aaggagctga tggcccgtct cggtcacacg
     6421 actcctagga tggcgatgaa gtaccagatg gcgtctgagg cccgcgacga ggctatcgct
     6481 gaggcgatgt ccaagctggc caagacctcc tgaaacgcaa aaagcccccc tcccaaggac
     6541 actgagtcct aaagaggggg gtttcttgtc agtacgcgaa gaaccacgcc tggccgcgag
     6601 cgccagcacc gccgctctgt gcggagacct gggcaccagc cccgccgccg ccaggagcat
     6661 tgccgttccc gccagaaatc tagacggtga ccacaacgcg cccgctttga tcggggacgt
     6721 cgaaatattg gatcgtcggc accgtcacgg ccgtgggagg cggcacgatc cgcgacgtga
     6781 tgatcggccg catccccacg gtgctgcgca gtgagctcta cgccatcccg gcgttgatct
     6841 gtgcgttcgc acgcacaggc ccggtgtgag aagggtctct gacgagcggg agaactccct
     6901 atcag
//
