LOCUS       sequence-316084-        5621 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0222:Dendra
FEATURES             Location/Qualifiers
     source          1..5621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1565..1588
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1845..2645
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2770..3358
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3259..3278
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgagcgacga caagatgctc gcccgcatcg cagcgctgct
      121 gcgacaggcc gagggcaccg acaatccaca cgaggcggag gccttcatgg ccgccgcgca
      181 gcggctggcc accgcgacgt ccatcgatct ggcgctggcg cgtgcgcact ccgacaaacg
      241 caccaaggcc cagatgccgg tgcagcgcac catcaccatc ggtcaggcag gcaccaaggg
      301 actgcgcacc tatgtgcagc tgttcgtgtt gatcgcgcag gccaacgacg tgaaatgcga
      361 cgtggcgtcc aactcgacgt tcgtctacgc ctacggcttc gccgaggaca tcgacgccac
      421 gcatgcgctg tacaccagcc tcgtgatgca gatggtacgc gcctcggagg cctacatcgc
      481 ctcgggtgcg caccggccga cgccgacgat caccgcgcgc ctgaacttcc agctcgcgtt
      541 cggcgcacgc gtcggccagc ggctggcaga ggcacgcgac gaggccaggc aggaggccga
      601 cgccgccgat cagccgggca ctgcgcttgc gttgcgcgac aaggacatcg agctgaagag
      661 cttctaccgg caggcgtcgc aggcgcgtgg cacgtggcgg gccacgagtg cgtccgcggg
      721 ctactcgtcg gcggcgcgac gtgccggtga ccgcgccggc cggcgtgcac ggctggggcc
      781 gatgcaggaa ctgtcgggcg cgcgtggcgc actggagaag aagcttggtg cggccgcggg
      841 caacacgcct ggcatcaatc tgatcaagga ggacatgcgt gtcaaggtgc atatggaggg
      901 caacgtgaac ggtcacgcct tcgtgatcga gggggagggt aagggtaagc cttacgaagg
      961 gacccagacc gccaacctca ccgtcaagga gggcgcccct ctcccattct cgtacgatat
     1021 cctcactacc gcggtgcatt acgggaaccg cgtgttcacc aagtaccccg aggatatccc
     1081 tgactacttc aagcagagct tcccggaggg atactcctgg gagcggacga tgaccttcga
     1141 agacaagggt atctgtacga tccgcagcga catctcgctg gaaggggact gtttcttcca
     1201 gaacgtccgt ttcaagggga ccaactttcc gccgaacggc ccggtcatgc agaagaagac
     1261 cctcaagtgg gagccgagca cggagaagct gcacgttcgg gacggcctcc tggtcggcaa
     1321 catcaacatg gcgctgctgc tggaaggcgg cggccactac ctatgcgact tcaagaccac
     1381 gtacaaagcg aagaaggtcg tccagctgcc cgacgcgcac ttcgtggacc accgcatcga
     1441 aatcctgggg aacgattcgg actacaacaa ggtcaagttg tacgagcacg ctgtggcacg
     1501 ctatagcccg ctcccgtcgc aggtctggtt cgaaggcggc ggcggggaga acctgtattt
     1561 ccaggactac aaggacgacg acgacaagtg atttaaataa ccccttgggg cctctaaacg
     1621 ggtcttgagg ggttttttgt taactagcgt acgatcgaat tcctgcagct cacggtaact
     1681 gatgccgtat ttgcagtacc agcgtacggc ccacagaatg atgtcacgct gaaaatgccg
     1741 gcctttgaat gggttcatgt gcagctccat cagcaaaagg ggatgataag tttatcacca
     1801 ccgactattt gcaacagtgc cgttgatcgt gctatgatcg actgatgtca tcagcggtgg
     1861 agtgcaatgt cgtgcaatac gaatggcgaa aagccgagct catcggtcag cttctcaacc
     1921 ttggggttac ccccggcggt gtgctgctgg tccacagctc cttccgtagc gtccggcccc
     1981 tcgaagatgg gccacttgga ctgatcgagg ccctgcgtgc tacgctgggt ccgggaggga
     2041 cgctcgtcat gccctcgtgg tcaggtctgg acgacgagcc gttcgatcct gccacgtcgc
     2101 ccgttacacc ggaccttgga gttgtctctg acacattctg gcgcctgcca aatgtaaagc
     2161 gcagcgccca tccatttgcc tttgcggcag cggggccaca ggcagagcag atcatctctg
     2221 atccattgcc cctgccacct tactcgcctg caagcccggt cgcccgtgtc catgaactcg
     2281 atgggcaggt acttctcctc ggcgtgggac acgatgccaa cacgacgctg catcttgccg
     2341 agttgatggc aaaggttccc tatggggtgc cgagacactg caccattctt caggatggca
     2401 agttggtacg cgtcgattat ctcgagaatg accactgctg tgagcgcttt gccttggcgg
     2461 acaggtggct caaggagaag agccttcaga aggaaggtcc agtcggtcat gcctttgctc
     2521 ggttgatccg ctcccgcgac attgtggcga cagccctggg tcaactgggc cgagatccgt
     2581 tgatcttcct gcatccgcca gaggcgggat gcgaagaatg cgatgccgct cgccagtcga
     2641 ttggctgagc tcatgagcgg agaacgagat gacgttggag gggcaaggtc gcgctgattg
     2701 ctggggcaac acgggggatc cactagttcc atgagcgtca gaccccgtag aaaagatcaa
     2761 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2821 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2881 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     2941 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3001 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3061 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3121 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3181 tcccgagggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3241 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3301 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3361 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3421 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3481 taccgctcgc cgcagccgaa cgaccgagcg caacgcgtgc ggccgcggta cccggggatc
     3541 ctctagagtc gaccaccaag ggcaccatct ctgcttgggc caccccgttg gccgcagcca
     3601 gctcgctgag agccgtgaac gacagggcga acgccagccc gccgacggcg agggttccga
     3661 ccgctgcaac tcccggtgca accttgtccc ggtctattct cttcactgca ccagctccaa
     3721 tctggtgtga atgcccctcg tctgttcgcg caggcggggg gctctattcg tttgtcagca
     3781 tcgaaagtag ccagatcagg gatgcgttgc aaccgcgtat gcccaggtca gaagagtcgc
     3841 acaagagttg cagacccctg gaaagaaaaa tggccagagg gcgaaaacac cctctgacca
     3901 gcggagcggg cgacgggaat cgaacccgcg tagctagttt ggaagaatgg gtgtctgccg
     3961 accacatatg ggccggtcaa gataggtttt taccccctct cggctgcatc ctctaagtgg
     4021 aaagaaattg caggtcgtag aagcgcgttg aagcctgaga gttgcacagg agttgcaacc
     4081 cggtagcctt gttcacgacg agaggagacc tagttggcac gtcgcggatg gggatcgctg
     4141 aagactcagc gcagcgggag gatccaagcc tcatacgtca acccgcagga cggtgtgagg
     4201 tactacgcgc tgcagaccta cgacaacaag atggacgccg aagcctggct cgcgggcgag
     4261 aagcggctca tcgagatgga gacctggacc cctccacagg accgggcgaa gaaggcagcc
     4321 gccagcgcca tcacgctgga ggagtacacc cggaagtggc tcgtggagcg cgacctcgca
     4381 gacggcacca gggatctgta cagcgggcac gcggagcgcc gcatctaccc ggtgctaggt
     4441 gaagtggcgg tcacagagat gacgccagct ctggtgcgtg cgtggtgggc cgggatgggt
     4501 aggaagcacc cgactgcccg ccggcatgcc tacaacgtcc tccgggcggt gatgaacaca
     4561 gcggtcgagg acaagctgat cgcagagaac ccgtgccgga tcgagcagaa ggcagccgat
     4621 gagcgcgacg tagaggcgct gacgcctgag gagctggaca tcgtcgccgc tgagatcttc
     4681 gagcactacc ggatcgcggc atacatcctg gcgtggacga gcctccggtt cggagagctg
     4741 atcgagcttc gccgcaagga catcgtggac gacggcatga cgatgaagct ccgggtgcgc
     4801 cgtggcgctt cccgcgtggg gaacaagatc gtcgttggca acgccaagac cgtccggtcg
     4861 aagcgtcctg tgacggttcc gcctcacgtc gcggagatga tccgagcgca catgaaggac
     4921 cgtacgaaga tgaacaaggg ccccgaggca ttcctggtga ccacgacgca gggcaaccgg
     4981 ctgtcgaagt ccgcgttcac caagtcgctg aagcgtggct acgccaagat cggtcggccg
     5041 gaactccgca tccacgacct ccgcgctgtc ggcgctacgt tcgccgctca ggcaggtgcg
     5101 acgaccaagg agctgatggc ccgtctcggt cacacgactc ctaggatggc gatgaagtac
     5161 cagatggcgt ctgaggcccg cgacgaggct atcgctgagg cgatgtccaa gctggccaag
     5221 acctcctgaa acgcaaaaag cccccctccc aaggacactg agtcctaaag aggggggttt
     5281 cttgtcagta cgcgaagaac cacgcctggc cgcgagcgcc agcaccgccg ctctgtgcgg
     5341 agacctgggc accagccccg ccgccgccag gagcattgcc gttcccgcca gaaatctaga
     5401 cggtgaccac aacgcgcccg ctttgatcgg ggacgtcgaa atattggatc gtcggcaccg
     5461 tcacggccgt gggaggcggc acgatccgcg acgtgatgat cggccgcatc cccacggtgc
     5521 tgcgcagtga gctctacgcc atcccggcgt tgatctgtgc gttcgcacgc acaggcccgg
     5581 tgtgagaagg gtctctgacg agcgggagaa ctccctatca g
//
