LOCUS       sequence-316085-        6293 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6293)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0032:Dendra
FEATURES             Location/Qualifiers
     source          1..6293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2237..2260
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2517..3317
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3442..4030
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3931..3950
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgacgacgca accccagtcg ccggttaccg tcacgccgcc
      121 gctcccgaac cggcgcaatg cggaactgct gctgctgggg ttcgcggcgc tgatcaccac
      181 cgtggccctg ttgatcgtcg aggccaacca ggagcagggc gtgacctggg atctggccca
      241 ctacatggtc ggctacctgg ccctgttcgg cgccgcgcac ctcgcggtcc ggcggttcgc
      301 gccgtacgcc gatccactgc tgctgccggt cgtcgccgtg ctgaacgggt tgggcctggt
      361 gatgatccac cggctcgacc ttgccgaggg tgagctgacc gccgacggcc tcggcggcag
      421 cgccaaccag cagatgctgt ggacgctggt cggtgtggtg gcgttcgcgc tcgtggtggt
      481 gttcttgaag gaccaccgca tgctgtcgcg ctacggctac gtcttcggtc tgaccggcct
      541 tgtgctgctt gtcattccgg cgctgctgcc ggccaagtac tccgagcagt acggcgccaa
      601 gatctggatt cagttcccgg gcttctcgat tcagcccgcc gagttctcca agattttgct
      661 gctcatcttc ttctccgcgg tgctggtgtc caagcgcagc ctgttcacca gcgcgggcaa
      721 gcacgtcctt ggcgtcgatc tgccccgccc ccgcgacctc gcaccgctgc tggtggcctg
      781 ggccgcgtcg gtgggcatca tgatcttcga aaaagacctc ggcacttcac ttctgctgta
      841 cgcgtcgttt ctggtgctgc tctacgtcgc caccgaacgg atcagctggg tggtcatcgg
      901 cctggcgctg ttcgcggcgg gaagtgtggt ggcctacaac attttcgacc acgtgcaggt
      961 gcgggtgcag acgtggctgg acccgttcgc cgaccccgag ggcgccggct accagatggt
     1021 gcagtcgttg ttcagcttcg ccacgggcgg catcttcggc accggcctgg gcaacggaca
     1081 gcccggcacc gtccctgccg ccgccaccga cttcatcacc gccgcgatcg gtgaggaact
     1141 cggattggtc ggtctggcag gcgttctcat gctctacacc atcctcatca tccgcggcct
     1201 gcgcacggcg atcgcggtgc gcgacagctt cggcaaactg ctcgcggcgg gtctggcgtc
     1261 gacactggcg atccagttgt tcatcgtcgt cggcggtgtg acgcggctga tcccgctgac
     1321 cggcctcacc acgccgtgga tgtcctacgg cgggtcgtcg ctgctggcga actacgtgct
     1381 gctcgcgatc ctcgtcgtga tctcgcacgc ggcccgcagg cccatcacca cgtcccggcc
     1441 gccgaacgcg acgccgatcg ccgcggccag taccgaggtg atcgaaaagg taaagcttgg
     1501 tgcggccgcg ggcaacacgc ctggcatcaa tctgatcaag gaggacatgc gtgtcaaggt
     1561 gcatatggag ggcaacgtga acggtcacgc cttcgtgatc gagggggagg gtaagggtaa
     1621 gccttacgaa gggacccaga ccgccaacct caccgtcaag gagggcgccc ctctcccatt
     1681 ctcgtacgat atcctcacta ccgcggtgca ttacgggaac cgcgtgttca ccaagtaccc
     1741 cgaggatatc cctgactact tcaagcagag cttcccggag ggatactcct gggagcggac
     1801 gatgaccttc gaagacaagg gtatctgtac gatccgcagc gacatctcgc tggaagggga
     1861 ctgtttcttc cagaacgtcc gtttcaaggg gaccaacttt ccgccgaacg gcccggtcat
     1921 gcagaagaag accctcaagt gggagccgag cacggagaag ctgcacgttc gggacggcct
     1981 cctggtcggc aacatcaaca tggcgctgct gctggaaggc ggcggccact acctatgcga
     2041 cttcaagacc acgtacaaag cgaagaaggt cgtccagctg cccgacgcgc acttcgtgga
     2101 ccaccgcatc gaaatcctgg ggaacgattc ggactacaac aaggtcaagt tgtacgagca
     2161 cgctgtggca cgctatagcc cgctcccgtc gcaggtctgg ttcgaaggcg gcggcgggga
     2221 gaacctgtat ttccaggact acaaggacga cgacgacaag tgatttaaat aaccccttgg
     2281 ggcctctaaa cgggtcttga ggggtttttt gttaactagc gtacgatcga attcctgcag
     2341 ctcacggtaa ctgatgccgt atttgcagta ccagcgtacg gcccacagaa tgatgtcacg
     2401 ctgaaaatgc cggcctttga atgggttcat gtgcagctcc atcagcaaaa ggggatgata
     2461 agtttatcac caccgactat ttgcaacagt gccgttgatc gtgctatgat cgactgatgt
     2521 catcagcggt ggagtgcaat gtcgtgcaat acgaatggcg aaaagccgag ctcatcggtc
     2581 agcttctcaa ccttggggtt acccccggcg gtgtgctgct ggtccacagc tccttccgta
     2641 gcgtccggcc cctcgaagat gggccacttg gactgatcga ggccctgcgt gctacgctgg
     2701 gtccgggagg gacgctcgtc atgccctcgt ggtcaggtct ggacgacgag ccgttcgatc
     2761 ctgccacgtc gcccgttaca ccggaccttg gagttgtctc tgacacattc tggcgcctgc
     2821 caaatgtaaa gcgcagcgcc catccatttg cctttgcggc agcggggcca caggcagagc
     2881 agatcatctc tgatccattg cccctgccac cttactcgcc tgcaagcccg gtcgcccgtg
     2941 tccatgaact cgatgggcag gtacttctcc tcggcgtggg acacgatgcc aacacgacgc
     3001 tgcatcttgc cgagttgatg gcaaaggttc cctatggggt gccgagacac tgcaccattc
     3061 ttcaggatgg caagttggta cgcgtcgatt atctcgagaa tgaccactgc tgtgagcgct
     3121 ttgccttggc ggacaggtgg ctcaaggaga agagccttca gaaggaaggt ccagtcggtc
     3181 atgcctttgc tcggttgatc cgctcccgcg acattgtggc gacagccctg ggtcaactgg
     3241 gccgagatcc gttgatcttc ctgcatccgc cagaggcggg atgcgaagaa tgcgatgccg
     3301 ctcgccagtc gattggctga gctcatgagc ggagaacgag atgacgttgg aggggcaagg
     3361 tcgcgctgat tgctggggca acacggggga tccactagtt ccatgagcgt cagaccccgt
     3421 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3481 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3541 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     3601 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3661 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3721 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3781 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3841 aagcgccacg cttcccgagg ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3901 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3961 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4021 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4081 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     4141 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcaacgcgt gcggccgcgg
     4201 tacccgggga tcctctagag tcgaccacca agggcaccat ctctgcttgg gccaccccgt
     4261 tggccgcagc cagctcgctg agagccgtga acgacagggc gaacgccagc ccgccgacgg
     4321 cgagggttcc gaccgctgca actcccggtg caaccttgtc ccggtctatt ctcttcactg
     4381 caccagctcc aatctggtgt gaatgcccct cgtctgttcg cgcaggcggg gggctctatt
     4441 cgtttgtcag catcgaaagt agccagatca gggatgcgtt gcaaccgcgt atgcccaggt
     4501 cagaagagtc gcacaagagt tgcagacccc tggaaagaaa aatggccaga gggcgaaaac
     4561 accctctgac cagcggagcg ggcgacggga atcgaacccg cgtagctagt ttggaagaat
     4621 gggtgtctgc cgaccacata tgggccggtc aagataggtt tttaccccct ctcggctgca
     4681 tcctctaagt ggaaagaaat tgcaggtcgt agaagcgcgt tgaagcctga gagttgcaca
     4741 ggagttgcaa cccggtagcc ttgttcacga cgagaggaga cctagttggc acgtcgcgga
     4801 tggggatcgc tgaagactca gcgcagcggg aggatccaag cctcatacgt caacccgcag
     4861 gacggtgtga ggtactacgc gctgcagacc tacgacaaca agatggacgc cgaagcctgg
     4921 ctcgcgggcg agaagcggct catcgagatg gagacctgga cccctccaca ggaccgggcg
     4981 aagaaggcag ccgccagcgc catcacgctg gaggagtaca cccggaagtg gctcgtggag
     5041 cgcgacctcg cagacggcac cagggatctg tacagcgggc acgcggagcg ccgcatctac
     5101 ccggtgctag gtgaagtggc ggtcacagag atgacgccag ctctggtgcg tgcgtggtgg
     5161 gccgggatgg gtaggaagca cccgactgcc cgccggcatg cctacaacgt cctccgggcg
     5221 gtgatgaaca cagcggtcga ggacaagctg atcgcagaga acccgtgccg gatcgagcag
     5281 aaggcagccg atgagcgcga cgtagaggcg ctgacgcctg aggagctgga catcgtcgcc
     5341 gctgagatct tcgagcacta ccggatcgcg gcatacatcc tggcgtggac gagcctccgg
     5401 ttcggagagc tgatcgagct tcgccgcaag gacatcgtgg acgacggcat gacgatgaag
     5461 ctccgggtgc gccgtggcgc ttcccgcgtg gggaacaaga tcgtcgttgg caacgccaag
     5521 accgtccggt cgaagcgtcc tgtgacggtt ccgcctcacg tcgcggagat gatccgagcg
     5581 cacatgaagg accgtacgaa gatgaacaag ggccccgagg cattcctggt gaccacgacg
     5641 cagggcaacc ggctgtcgaa gtccgcgttc accaagtcgc tgaagcgtgg ctacgccaag
     5701 atcggtcggc cggaactccg catccacgac ctccgcgctg tcggcgctac gttcgccgct
     5761 caggcaggtg cgacgaccaa ggagctgatg gcccgtctcg gtcacacgac tcctaggatg
     5821 gcgatgaagt accagatggc gtctgaggcc cgcgacgagg ctatcgctga ggcgatgtcc
     5881 aagctggcca agacctcctg aaacgcaaaa agcccccctc ccaaggacac tgagtcctaa
     5941 agaggggggt ttcttgtcag tacgcgaaga accacgcctg gccgcgagcg ccagcaccgc
     6001 cgctctgtgc ggagacctgg gcaccagccc cgccgccgcc aggagcattg ccgttcccgc
     6061 cagaaatcta gacggtgacc acaacgcgcc cgctttgatc ggggacgtcg aaatattgga
     6121 tcgtcggcac cgtcacggcc gtgggaggcg gcacgatccg cgacgtgatg atcggccgca
     6181 tccccacggt gctgcgcagt gagctctacg ccatcccggc gttgatctgt gcgttcgcac
     6241 gcacaggccc ggtgtgagaa gggtctctga cgagcgggag aactccctat cag
//
