LOCUS       sequence-316086-        6704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0255:Dendra
FEATURES             Location/Qualifiers
     source          1..6704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2648..2671
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2928..3728
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3853..4441
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4342..4361
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgacctcagc gaccattccg ggtttggaca ccgcaccgac
      121 taaacatcag ggactgctgg cgtgggtcca ggaggttgcg gagctgacgc agccggatcg
      181 ggtggtgttc gccgacggct ccgacgagga gtacgagcgg ctgtgcgccc acctcgtcga
      241 ggccggcacc ttccagaagc tcaatcccga gaagcagccc aattcgtacc tcgcgctttc
      301 cgatccgtcc gacgtcgcgc gtgtcgaatc ccgcaccttc atctgcaccg agcgtgagat
      361 cgacgcgggt ccgaccaaca actggatgga cccggccgag atgcgcggca tcatgaccga
      421 cctgtaccgg ggctcgatgc gcggccgcac cctctacgtc gtgccgttct gcatgggccc
      481 gctggatgcc gaggacccca agctcggcgt cgagatcacc gactcggagt acgtcgtcgt
      541 ctcgatgcgc accatgaccc gcatgggccg ggccgcgttg gacaagctcg gtgacgacgg
      601 cttcttcgtc aaggccctgc actcgatcgg cgctccgctc gagcccggtc agaaggacgt
      661 gccgtggccg tgcaacgaca ccaagtacat cacccacttt cccgagaccc gtgagatctg
      721 gagcttcggc tcgggatacg gcggcaacgc gctgctgggc aagaagtgct actcgctgcg
      781 catcgcgtcg gccatggccc acgacgaggg ctggctcgcc gagcacatgc tgatcctcaa
      841 gctgatcagc cccgagaaca aggcgtactt catcgccgcg gcgttcccgt cggcgtgcgg
      901 caagaccaac ctcgcgatgc tgcagcccac cattgaaggc tggcgcgccg agaccgtcgg
      961 cgacgacatc gcgtggatgc gcttcggcaa ggacggccgc ctgtacgcga ccaaccccga
     1021 gttcggtttc ttcggcgtcg cgcccggcac caactggtcg tcgaacccca acgccatgaa
     1081 gaccatcgcc gcgggcaaca ccgtgttcac caacgtcgcc aagaccgacg acggtgacgt
     1141 gtggtgggag ggcctcgagg gcgacccgca gcacctcatc gactggaagg gcaacgactg
     1201 gacgccggag tccggtgaga aggccgccca ccccaactcg cggtactgca ccccgatctc
     1261 gcagtgcccg acgctggcgc ccgagtggga cgacccgcag ggtgtgccga tctcggcgat
     1321 cctgttcggc ggccgccgca agaccacggt cccgctgatc accgaggccc gcgactggca
     1381 gcacggcgtg ttcatcggtg ccaccctggg ttcggagcag accgccgcag ccgagggcaa
     1441 ggtgggcacc gtgcgccgcg acccgatggc catgctgccg ttcctgggct acaacgtcgg
     1501 tgactacttc gcgcactgga tcaacgtggg caagaacgcc gacgagtcca agctgcccaa
     1561 ggtgttcttc gtcaactggt tccgtcgcgg cgacgacggc cgcttcctgt ggccgggctt
     1621 cggcgagaac agccgcgtgc tcaagtgggc cgtcgagcgc atcgagcaca aggccgacgg
     1681 taagagcacc ccgatcggca tcgtgccgac cgcggccgac ctcgacctcg aaggtctcga
     1741 tgtggacccc gccgacgtcg acgaggcgct cgccgtcaag cccgaggagt ggcgtgccga
     1801 gctcccgttg atcgaggagt ggttcgagtt cgtcggtgag aagctgccga ccggcctcaa
     1861 ggacgagttc gacgcgctca agcaccgcct gtccgaagag ggcaagcttg gtgcggccgc
     1921 gggcaacacg cctggcatca atctgatcaa ggaggacatg cgtgtcaagg tgcatatgga
     1981 gggcaacgtg aacggtcacg ccttcgtgat cgagggggag ggtaagggta agccttacga
     2041 agggacccag accgccaacc tcaccgtcaa ggagggcgcc cctctcccat tctcgtacga
     2101 tatcctcact accgcggtgc attacgggaa ccgcgtgttc accaagtacc ccgaggatat
     2161 ccctgactac ttcaagcaga gcttcccgga gggatactcc tgggagcgga cgatgacctt
     2221 cgaagacaag ggtatctgta cgatccgcag cgacatctcg ctggaagggg actgtttctt
     2281 ccagaacgtc cgtttcaagg ggaccaactt tccgccgaac ggcccggtca tgcagaagaa
     2341 gaccctcaag tgggagccga gcacggagaa gctgcacgtt cgggacggcc tcctggtcgg
     2401 caacatcaac atggcgctgc tgctggaagg cggcggccac tacctatgcg acttcaagac
     2461 cacgtacaaa gcgaagaagg tcgtccagct gcccgacgcg cacttcgtgg accaccgcat
     2521 cgaaatcctg gggaacgatt cggactacaa caaggtcaag ttgtacgagc acgctgtggc
     2581 acgctatagc ccgctcccgt cgcaggtctg gttcgaaggc ggcggcgggg agaacctgta
     2641 tttccaggac tacaaggacg acgacgacaa gtgatttaaa taaccccttg gggcctctaa
     2701 acgggtcttg aggggttttt tgttaactag cgtacgatcg aattcctgca gctcacggta
     2761 actgatgccg tatttgcagt accagcgtac ggcccacaga atgatgtcac gctgaaaatg
     2821 ccggcctttg aatgggttca tgtgcagctc catcagcaaa aggggatgat aagtttatca
     2881 ccaccgacta tttgcaacag tgccgttgat cgtgctatga tcgactgatg tcatcagcgg
     2941 tggagtgcaa tgtcgtgcaa tacgaatggc gaaaagccga gctcatcggt cagcttctca
     3001 accttggggt tacccccggc ggtgtgctgc tggtccacag ctccttccgt agcgtccggc
     3061 ccctcgaaga tgggccactt ggactgatcg aggccctgcg tgctacgctg ggtccgggag
     3121 ggacgctcgt catgccctcg tggtcaggtc tggacgacga gccgttcgat cctgccacgt
     3181 cgcccgttac accggacctt ggagttgtct ctgacacatt ctggcgcctg ccaaatgtaa
     3241 agcgcagcgc ccatccattt gcctttgcgg cagcggggcc acaggcagag cagatcatct
     3301 ctgatccatt gcccctgcca ccttactcgc ctgcaagccc ggtcgcccgt gtccatgaac
     3361 tcgatgggca ggtacttctc ctcggcgtgg gacacgatgc caacacgacg ctgcatcttg
     3421 ccgagttgat ggcaaaggtt ccctatgggg tgccgagaca ctgcaccatt cttcaggatg
     3481 gcaagttggt acgcgtcgat tatctcgaga atgaccactg ctgtgagcgc tttgccttgg
     3541 cggacaggtg gctcaaggag aagagccttc agaaggaagg tccagtcggt catgcctttg
     3601 ctcggttgat ccgctcccgc gacattgtgg cgacagccct gggtcaactg ggccgagatc
     3661 cgttgatctt cctgcatccg ccagaggcgg gatgcgaaga atgcgatgcc gctcgccagt
     3721 cgattggctg agctcatgag cggagaacga gatgacgttg gaggggcaag gtcgcgctga
     3781 ttgctggggc aacacggggg atccactagt tccatgagcg tcagaccccg tagaaaagat
     3841 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3901 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3961 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     4021 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4081 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4141 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4201 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4261 gcttcccgag gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4321 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4381 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4441 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4501 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     4561 tgataccgct cgccgcagcc gaacgaccga gcgcaacgcg tgcggccgcg gtacccgggg
     4621 atcctctaga gtcgaccacc aagggcacca tctctgcttg ggccaccccg ttggccgcag
     4681 ccagctcgct gagagccgtg aacgacaggg cgaacgccag cccgccgacg gcgagggttc
     4741 cgaccgctgc aactcccggt gcaaccttgt cccggtctat tctcttcact gcaccagctc
     4801 caatctggtg tgaatgcccc tcgtctgttc gcgcaggcgg ggggctctat tcgtttgtca
     4861 gcatcgaaag tagccagatc agggatgcgt tgcaaccgcg tatgcccagg tcagaagagt
     4921 cgcacaagag ttgcagaccc ctggaaagaa aaatggccag agggcgaaaa caccctctga
     4981 ccagcggagc gggcgacggg aatcgaaccc gcgtagctag tttggaagaa tgggtgtctg
     5041 ccgaccacat atgggccggt caagataggt ttttaccccc tctcggctgc atcctctaag
     5101 tggaaagaaa ttgcaggtcg tagaagcgcg ttgaagcctg agagttgcac aggagttgca
     5161 acccggtagc cttgttcacg acgagaggag acctagttgg cacgtcgcgg atggggatcg
     5221 ctgaagactc agcgcagcgg gaggatccaa gcctcatacg tcaacccgca ggacggtgtg
     5281 aggtactacg cgctgcagac ctacgacaac aagatggacg ccgaagcctg gctcgcgggc
     5341 gagaagcggc tcatcgagat ggagacctgg acccctccac aggaccgggc gaagaaggca
     5401 gccgccagcg ccatcacgct ggaggagtac acccggaagt ggctcgtgga gcgcgacctc
     5461 gcagacggca ccagggatct gtacagcggg cacgcggagc gccgcatcta cccggtgcta
     5521 ggtgaagtgg cggtcacaga gatgacgcca gctctggtgc gtgcgtggtg ggccgggatg
     5581 ggtaggaagc acccgactgc ccgccggcat gcctacaacg tcctccgggc ggtgatgaac
     5641 acagcggtcg aggacaagct gatcgcagag aacccgtgcc ggatcgagca gaaggcagcc
     5701 gatgagcgcg acgtagaggc gctgacgcct gaggagctgg acatcgtcgc cgctgagatc
     5761 ttcgagcact accggatcgc ggcatacatc ctggcgtgga cgagcctccg gttcggagag
     5821 ctgatcgagc ttcgccgcaa ggacatcgtg gacgacggca tgacgatgaa gctccgggtg
     5881 cgccgtggcg cttcccgcgt ggggaacaag atcgtcgttg gcaacgccaa gaccgtccgg
     5941 tcgaagcgtc ctgtgacggt tccgcctcac gtcgcggaga tgatccgagc gcacatgaag
     6001 gaccgtacga agatgaacaa gggccccgag gcattcctgg tgaccacgac gcagggcaac
     6061 cggctgtcga agtccgcgtt caccaagtcg ctgaagcgtg gctacgccaa gatcggtcgg
     6121 ccggaactcc gcatccacga cctccgcgct gtcggcgcta cgttcgccgc tcaggcaggt
     6181 gcgacgacca aggagctgat ggcccgtctc ggtcacacga ctcctaggat ggcgatgaag
     6241 taccagatgg cgtctgaggc ccgcgacgag gctatcgctg aggcgatgtc caagctggcc
     6301 aagacctcct gaaacgcaaa aagcccccct cccaaggaca ctgagtccta aagagggggg
     6361 tttcttgtca gtacgcgaag aaccacgcct ggccgcgagc gccagcaccg ccgctctgtg
     6421 cggagacctg ggcaccagcc ccgccgccgc caggagcatt gccgttcccg ccagaaatct
     6481 agacggtgac cacaacgcgc ccgctttgat cggggacgtc gaaatattgg atcgtcggca
     6541 ccgtcacggc cgtgggaggc ggcacgatcc gcgacgtgat gatcggccgc atccccacgg
     6601 tgctgcgcag tgagctctac gccatcccgg cgttgatctg tgcgttcgca cgcacaggcc
     6661 cggtgtgaga agggtctctg acgagcggga gaactcccta tcag
//
