LOCUS       sequence-316088-       23386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BioBrick encoding cistrons 22-30 in pET for preparation of PURE 3.0 
            translation system from E. coli .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 23386)
  AUTHORS   Shepherd TR, Du L, Liljeruhm J, Samudyata, Wang J, Sjodin MOD, 
            Wetterhall M, Yomo T, Forster AC
  TITLE     De novo design and synthesis of a 30-cistron translation-factor 
            module.
  JOURNAL   Nucleic Acids Res. 2017 Oct 13;45(18):10895-10905. doi: 
            10.1093/nar/gkx753.
  PUBMED    28977654
REFERENCE   2  (bases 1 to 23386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2017-10-13- 13"; volume: "45"; issue: 
            "18"; pages: "10895-10905"
COMMENT     Sequence Label: pLD3 translation factors
FEATURES             Location/Qualifiers
     source          1..23386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      8..55
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        92..110
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    111..135
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             150..172
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1896..1913
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      1999..2046
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        2083..2101
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2102..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2141..2163
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5159..5176
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      5262..5309
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5346..5364
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5365..5389
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5404..5426
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             6847..6864
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      6950..6997
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        7034..7052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    7053..7077
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             7092..7114
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             7125..7142
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      8812..8859
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        8896..8914
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    8915..8939
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             8954..8976
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             10079..10096
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      10182..10229
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        10266..10284
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    10285..10309
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             10324..10346
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             10909..10926
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      11012..11059
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        11096..11114
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    11115..11139
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             11154..11176
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             13296..13313
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      13399..13446
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        13483..13501
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    13502..13526
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             13541..13563
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             13574..13591
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      16344..16391
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    16406..16426
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     rep_origin      16445..16900
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(16532..16551)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     16742..16763
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(16992..17807)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(16992..17807)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     17715..17734
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     17799..17818
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      17929..18515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     18416..18435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     18669..18686
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    18701..18840
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(18766..18785)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     18926..18948
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(18942..19133)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(19942..21024)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     20986..21005
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(21025..21102)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     21308..21327
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     misc_feature    21397..21418
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        21433..21451
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    21452..21476
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             21491..21513
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             23291..23308
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
       61 aggaggaact actagagcga tcccgcgaaa ttaatacgac tcactatagg ggaattgtga
      121 gcggataaca attccccact agaaataatt ttgtttaact ttaagaagga gatatacata
      181 tgcgtactag ccaatacctg ctctccactc tcaaggagac acctgccgac gccgaggtga
      241 tcagccatca gctgatgctg cgcgccggga tgatccgcaa gctggcctcc gggttatata
      301 cctggctgcc gaccggcgtg cgcgttctga aaaaagtcga aaacatcgtg cgtgaagaga
      361 tgaacaacgc cggtgcgatc gaggtgtcga tgccggtggt tcagccagcc gatttgtggc
      421 aagagagtgg tcgttgggaa cagtacggtc cggaactgct gcgttttgtt gaccgtggcg
      481 agcgtccgtt cgtactcggc ccaactcatg aagaagttat cactgacctg attcgtaacg
      541 agcttagctc ttacaaacag ctgccgctga acttctatca gatccagacc aagttccgcg
      601 acgaagtgcg tccgcgtttc ggcgtcatgc gttcccgcga gttcctgatg aaagatgctt
      661 actctttcca tacttctcag gaatccctgc aagaaaccta cgatgcaatg tatgcggcct
      721 acagcaaaat cttcagccgc atggggctgg atttccgcgc cgtacaagcc gacaccggtt
      781 ctatcggcgg cagcgcctct cacgagttcc aggtgctggc gcagagcggt gaagacgatg
      841 tggtcttctc cgacacctct gactatgcag cgaacattga actggcagaa gctatcgcgc
      901 cgaaagaacc gcgcgctgct gctacccagg aaatgacgct ggttgatacg ccgaacgcga
      961 aaaccatcgc ggaactggtt gaacagttca atctgccgat tgagaaaacg gttaagactc
     1021 tgctggttaa agcggttgaa ggcagcagct tcccgcaggt tgcgctgctg gtgcgcggtg
     1081 atcacgagct gaacgaagtt aaagcagaaa aactgccgca ggttgcaagc ccgctgactt
     1141 tcgcgaccga agaagaaatc cgtgccgtgg ttaaagccgg tccgggttca ctgggtccgg
     1201 taaacatgcc gattccggtg gtgattgacc gtaccgttgc ggcgatgagt gatttcgctg
     1261 ctggtgctaa catcgatggt aaacactact tcggcatcaa ctgggatcgc gatgtcgcta
     1321 ccccggaagt tgcagatatc cgtaacgtgg tggctggcga tccaagcccg gatggccagg
     1381 gtaggctgct gatcaaacgt ggtatcgaag ttggtcacat cttccagctg ggtaccaagt
     1441 actccgaagc actgaaagcc tccgtacagg gtgaagatgg ccgtaaccaa atcctgacga
     1501 tgggttgcta cggtatcggg gtaacgcgtg tggtagctgc ggcgattgag cagaactacg
     1561 acgaacgagg catcgtatgg cctgacgcta tcgcgccgtt ccaggtggcg attctgccga
     1621 tgaacatgca caaatccttc cgcgtacaag agcttgctga gaaactgtac agcgaactgc
     1681 gtgcacaagg tatcgaagtg ctgctggatg accgcaaaga gcgtccgggc gtgatgtttg
     1741 ctgatatgga actgatcggt attccgcaca ctattgtgct gggcgaccgt aacctcgaca
     1801 acgacgatat cgaatataaa tatcgtcgca acggcgagaa acagttaatt aagactggtg
     1861 acatcgtcga atatctggtg aaacagatta aaggccacca ccaccaccac cactaattat
     1921 ctgttagttt ttttctacta gactgctaac aaagcccgaa aggaagctga gttggctgct
     1981 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
     2041 tttttgctga aaggaggaac tactagagcg atcccgcgaa attaatacga ctcactatag
     2101 gggaattgtg agcggataac aattccccac tagaaataat tttgtttaac tttaagaagg
     2161 agatatacat atgcaaaagt ttgataccag gaccttccag ggcttgatcc tgaccttaca
     2221 ggattactgg gctcgccagg gctgcaccat tgttcaacca ttggacatgg aagtcggcgc
     2281 gggaacctct cacccaatga cctgtctgcg cgagctgggg ccagaaccga tggcggctgc
     2341 ttatgttcag ccttctcgtc gcccgaccga tggtcgctac ggcgaaaacc ccaaccgttt
     2401 acagcactac tatcagttcc aggtggtcat taagccatcg ccggacaata ttcaggagct
     2461 gtacctcggt tctctgaaag agctgggcat ggacccgact attcacgaca tccgtttcgt
     2521 ggaagataac tgggaaaacc cgacgctggg tgcctgggga ctgggctggg aagtgtggct
     2581 gaacggcatg gaagtgacgc agttcactta cttccagcag gttggtggtc tggagtgtaa
     2641 accggttacc ggcgagatca cctacggtct ggaacgtctg gccatgtaca ttcagggcgt
     2701 agacagcgtt tacgacctgg tctggagcga cggcccgctg ggtaaaacca cctacggcga
     2761 cgtgttccat cagaacgaag tggagcagtc cacttacaac ttcgaatacg cggatgtgga
     2821 cttcctgttc acctgcttcg agcagtacga gaaagaagcg cagcagctgc tggcgctgga
     2881 aaatccgctg ccgctgccag cctacgagcg tattctgaaa gccgcccaca gcttcaacct
     2941 gctggatgcg cgtaaagcca tctccgtcac cgagcgtcag cgctacattc tgcgcattcg
     3001 caccctgacc aaagcagtgg cagaagcata ctacgcttcc cgtgaagccc tcggcttccc
     3061 gatgtgcaac aaagataagt aagaggcggc tatgtctgag aaaacttttc tggtggaaat
     3121 cggcactgaa gagctgccac caaaagcact gcgcagcctg gctgagtcct ttgctgcgaa
     3181 ctttactgcg gagctggata acgctggcct cgcacacggc accgttcaat ggtttgctgc
     3241 tccgcgtcgt ctggcgctga aagtagctaa cctggcggaa gcgcaaccgg atcgtgaaat
     3301 cgaaaaacgc ggcccggcga ttgcccaggc gttcgacgct gaaggcaaac cgagcaaagc
     3361 ggcagaaggt tgggcgcgtg gttgcggtat taccgttgac caggctgagc gtctgactac
     3421 cgataaaggc gaatggctgc tgtatcgcgc ccatgtgaag ggcgaaagca ccgaagcact
     3481 gctgccgaat atggttgcga cttctctggc gaaactgccg atcccgaaac tgatgcgttg
     3541 gggcgcaagc gacgtgcact tcgtgcgtcc ggtgcacacc gtgaccctgc tgctgggcga
     3601 caaagtcatt ccggcaacca ttctgggcat tcagtccgat cgcgtgattc gcggccaccg
     3661 ctttatgggc gagccggagt tcaccatcga taacgccgat cagtatccgg aaatcctgcg
     3721 tgagcgtggg aaagtcatcg ccgattacga agaacgtaag gcgaagatta aagccgatgc
     3781 cgaagaagca gcgcgtaaga ttggcggtaa cgctgactta agcgaaagcc tgctggaaga
     3841 agtggcttcg ctggtggagt ggccggtcgt tctgaccgca aagttcgaag agaagttcct
     3901 cgcggtgccg gctgaagcgc tggtttacac catgaaaggt gaccagaaat acttcccggt
     3961 gtatgcgaac gacggcaaac tgctgccgaa ctttatcttc gttgccaaca tcgaatcgaa
     4021 agatccgcag cagattatct ccggtaacga gaaagtcgtt cgtccgcgtc tggcggatgc
     4081 cgagttcttc ttcaacaccg accgtaaaaa acgtcttgaa gataacctgc cgcgcctgca
     4141 aaccgtgttg ttccagcaac agttggggac gctgcgcgac aaaactgacc gcatccaggc
     4201 gctggctggc tggattgctg aacagattgg cgctgacgtt aaccacgcta cccgtgcggg
     4261 tctgctgtct aagtgcgacc tgatgaccaa catggtcttc gagttcaccg acacccaggg
     4321 cgttatgggg atgcactatg cgcgtcacga tggcgaagcg gaagatgtcg cggtggcgct
     4381 gaatgagcag tatcagccgc gttttgctgg tgatgacctg ccgtccaacc cagtagcttg
     4441 tgcgctggcg attgctgaca agatggatac cctggcgggt atcttcggta tcggtcagca
     4501 tccgaaaggc gacaaagacc cgtttgcgct gcgtcgtgcc gcgcttggcg tgctgcgaat
     4561 tatcgttgag aagaacctca accttgatct gcaaacgctg accgaagaag cggtgcgtct
     4621 gtatggcgat aagctgacta atgccaacgt agttgatgat gttatcgact ttatgctcgg
     4681 tcgcttccgc gcctggtatc aggacgaagg ttataccgtt gacaccatcc aggcggtact
     4741 ggcgcgtcgt ccgactcgtc cggctgattt cgatgcccgt atgaaagcgg tatcgcattt
     4801 ccgtaccctg gatgcagctg ctgcactggc ggcggcgaac aaacgtgtat ctaacattct
     4861 ggcgaaatct gacgaagtgc tgagcgaccg cgtgaatgcc tctaccctga aagagccgga
     4921 agaaattaaa ctggcgatgc aggttgtggt gctacgtgac aagctggagc cgtactttac
     4981 ggaaggtcgt taccaggatg cgctggtcga actggctgag ctgcgtgaac cggttgatgc
     5041 tttcttcgat aaagtgatgg tcatggttga tgacaaagaa ttgcgtatca accgtctgac
     5101 catgctggag aaactgcgcg aactgttcct gcgcgttgcg gatatttcgc tgttgcaaca
     5161 ccaccaccac caccactaat tatctgttag tttttttcta ctagactgct aacaaagccc
     5221 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
     5281 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactactaga gcgatcccgc
     5341 gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc cactagaaat
     5401 aattttgttt aactttaaga aggagatata catatgaaaa tcaaaactcg cttcgcgcca
     5461 agcccaacag gctatctgca cgttggcggc gcgcgtactg ctctttactc ctggcttttt
     5521 gcacgtaacc acggcggtga gttcgtgctg cgtattgaag acaccgatct tgagcgttcc
     5581 acgccggaag ctatcgaagc cattatggat ggcatgaact ggctgagcct ggagtgggat
     5641 gaaggtccgt actaccagac caaacgtttt gatcgctaca acgcggtgat cgatcagatg
     5701 ctggaagagg gcactgctta taaatgctat tgctctaaag agcgcctgga agcgctgcgc
     5761 gaagagcaaa tggcgaaagg tgagaagccg cgttatgacg gtcgctgccg ccacagccat
     5821 gagcatcatg ctgatgatga accgtgtgtt gtacgttttg ctaacccgca ggaaggttct
     5881 gttgtttttg acgatcagat ccgtggtccg atcgagttca gcaaccagga actggacgat
     5941 cttattatcc gccgtaccga tggttcccca acctataact tctgtgtggt tgtcgatgac
     6001 tgggatatgg aaatcaccca cgttatccgt ggcgaagacc atatcaacaa cacgccacgc
     6061 cagatcaaca ttcttaaggc cctgaaagcg ccggtgccgg tttacgcgca cgtttctatg
     6121 atcaatggcg atgacggtaa aaaactgtcc aaacgtcacg gggcagtcag cgtaatgcag
     6181 tatcgtgatg acggttattt gccagaagca ctgctgaact atctggtgcg tctgggctgg
     6241 tcccacggcg atcaggaaat cttcactcgt gaagagatga tcaaatactt cactttgaat
     6301 gccgtcagca aatctgccag tgcgttcaac accgacaagc tgctgtggct gaaccatcac
     6361 tacattaacg cgctgccgcc ggagtatgtt gctactcact tacagtggca cattgagcag
     6421 gaaaatatcg atacccgtaa cggcccgcag ctggctgatc tggtgaaact gctgggcgaa
     6481 cgctgcaaga cgctgaaaga gatggcacag agctgccgtt atttctacga agattttgct
     6541 gagttcgatg ccgacgccgc gaaaaaacat ctgcgtccgg tagcgcgtca gccgctggaa
     6601 gtggttcgtg acaaactggc cgcgattact gactggaccg ctgaaaacgt tcatcacgct
     6661 attcaggcga cggcggatga gctggaagtg ggtatgggta aagttggtat gccgctgcgt
     6721 gtcgccgtaa ccggtgcggg gcagtctcca gcactggatg ttaccgttca cgcaatcggt
     6781 aagacccgca gtatcgagcg tatcaacaaa gcgctggatt ttattgctga acgcgaaaat
     6841 cagcagcacc accaccacca ccactaatta tctgttagtt tttttctact agactgctaa
     6901 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
     6961 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctactagagc
     7021 gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccca
     7081 ctagaaataa ttttgtttaa ctttaagaag gagatataca tatgcaccac caccaccacc
     7141 acacgttgtc tccttatttg caagaggtgg cgaagcgccg cacttttgcc attatttctc
     7201 acccggacgc cggtaagact accatcaccg agaaggtgct gctgttcgga caggccattc
     7261 agaccgccgg tacagtaaaa ggccgtggtt ccaaccagca cgctaagtcg gactggatgg
     7321 agatggaaaa gcagcgtggg atctccatta ctacgtctgt gatgcagttt ccgtatcacg
     7381 attgcctggt taacctgctc gacaccccgg ggcacgaaga cttctcggaa gatacctatc
     7441 gtaccctgac ggcggtggac tgctgcctga tggttatcga cgccgcaaaa ggtgttgaag
     7501 atcgtacccg taagctgatg gaagttaccc gtctgcgcga cacgccgatc ctcaccttta
     7561 tgaacaaact tgaccgtgat atccgcgacc cgatggagct gctcgatgaa gttgagaacg
     7621 agctgaaaat cggctgtgcg ccgatcacct ggccgattgg ctgcggcaag ctgtttaaag
     7681 gcgtttacca cctttataaa gacgaaacct atctctatca gagcggtaaa ggccacacca
     7741 ttcaggaagt ccgcattgtt aaagggctga ataacccgga tctcgatgct gcggttggtg
     7801 aagatctggc acagcagctg cgtgacgaac tggaactggt gaaaggcgcg tctaacgagt
     7861 tcgacaaaga gctgttcctt gcgggcgaaa tcactccggt attcttcggt actgcgctgg
     7921 gtaacttcgg cgtcgatcac atgttggatg gcctggtgga gtgggcacct gcgccgatgc
     7981 cgcgtcagac tgatacccgt accgtagaag cgagcgaaga taagtttacc ggcttcgtat
     8041 ttaaaatcca ggccaacatg gacccgaaac accgcgaccg cgtggcgttt atgcgtgtgg
     8101 tgtccggtaa atatgaaaaa ggcatgaaac tgcgccaggt gcgcactgcg aaagatgtgg
     8161 tgatctccga cgcgctgacc tttatggcgg gtgaccgttc gcacgttgaa gaagcgtatc
     8221 cgggcgatat cctcggcctg cacaaccacg gcaccattca gatcggcgac acctttaccc
     8281 agggtgagat gatgaagttc accggtattc cgaacttcgc accagaactg ttccgtcgta
     8341 tccgcctgaa agatccgctg aagcaaaaac agctgctcaa agggctggta cagctttccg
     8401 aagagggcgc ggtgcaggtg ttccgtccaa tctccaacaa cgatctgatc gttggtgcag
     8461 ttggtgtgct gcaatttgat gtggtggtag cgcgcctgaa gagcgaatac aacgttgaag
     8521 cagtgtatga gtcagtcaac gttgccactg cccgctgggt agaatgtgca gacgcgaaga
     8581 agttcgaaga gttcaagcgt aagaacgaaa gccaactggc gcttgatggc ggcgataacc
     8641 tcgcttacat cgctaccagc atggtcaacc tgcgcctggc acaggaacgt tatccggacg
     8701 ttcagttcca ccagacccgc gagcattaat tatctgttag tttttttcta ctagactgct
     8761 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
     8821 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactactaga
     8881 gcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc
     8941 cactagaaat aattttgttt aactttaaga aggagatata catatgtttg aaattaatcc
     9001 ggtaaataat cgcattcagg acctcacgga acgctccgac gttcttaggg ggtatcttga
     9061 ctacgacgcc aagaaagagc gtctggaaga agtaaacgcc gagctggaac agccggatgt
     9121 ctggaacgaa cccgaacgcg cacaggcgct gggtaaagag cgttcctccc tcgaagccgt
     9181 tgtcgacacc ctcgaccaaa tgaaacaggg gctggaagat gtttctggtc tgctggaact
     9241 ggctgtagaa gctgacgacg aagaaacctt taacgaagcc gttgctgaac tcgacgccct
     9301 ggaagaaaaa ctggcgcagc ttgagttccg ccgtatgttc tctggcgaat atgacagcgc
     9361 cgactgctac ctcgatattc aggcggggtc tggcggtacg gaagcacagg actgggcgag
     9421 catgcttgag cgtatgtatc tgcgctgggc agaatcgcgt ggtttcaaaa ctgaaatcat
     9481 cgaagagtcg gaaggtgaag tggcgggtat taaatccgtg acgatcaaaa tctccggcga
     9541 ttacgcttac ggctggctgc gtacagaaac cggcgttcac cgcctggtgc gtaaaagccc
     9601 gtttgactcc ggcggtcgtc gccacacgtc gttcagctcc gcgtttgttt atccggaagt
     9661 tgatgatgat attgatatcg aaatcaaccc ggcggatctg cgcattgacg tttatcgcgc
     9721 gtccggcgcg ggcggtcagc acgttaaccg taccgaatct gcggtgcgta ttacccacat
     9781 cccgaccggg atcgtgaccc agtgccagaa cgaccgttcc cagcacaaga acaaagatca
     9841 ggccatgaag cagatgaaag cgaagcttta tgaactggag atgcagaaga aaaatgccga
     9901 gaaacaggcg atggaagata acaaatccga catcggctgg ggcagccaga ttcgttctta
     9961 tgtccttgat gactcccgca ttaaagatct gcgcaccggg gtagaaaccc gcaacacgca
    10021 ggccgtgctg gacggcagcc tggatcaatt tatcgaagca agtttgaaag cagggttaca
    10081 ccaccaccac caccactaat tatctgttag tttttttcta ctagactgct aacaaagccc
    10141 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
    10201 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactactaga gcgatcccgc
    10261 gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc cactagaaat
    10321 aattttgttt aactttaaga aggagatata catatgatta gcgatatcag aaaagatgct
    10381 gaagtacgca tggacaaatg cgtagaagcg ttcaaaaccc aaatcagcaa aatacgcacg
    10441 ggtcgtgctt ctcccagcct gctggatggc attgtcgtgg aatattacgg cacgccgacg
    10501 ccgctgcgtc agctggcaag cgtaacggta gaagattccc gtacactgaa aatcaacgtg
    10561 tttgatcgtt caatgtctcc ggccgttgaa aaagcgatta tggcgtccga tcttggcctg
    10621 aacccgaact ctgcgggtag cgacatccgt gttccgctgc cgccgctgac ggaagaacgt
    10681 cgtaaagatc tgaccaaaat cgttcgtggt gaagcagaac aagcgcgtgt tgcagtacgt
    10741 aacgtgcgtc gtgacgcgaa cgacaaagtg aaagcactgt tgaaagataa agagatcagc
    10801 gaagacgacg atcgccgttc tcaggacgat gtacagaaac tgactgatgc tgcaatcaag
    10861 aaaattgaag cggcgctggc agacaaagaa gcagaactga tgcagttcca ccaccaccac
    10921 caccactaat tatctgttag tttttttcta ctagactgct aacaaagccc gaaaggaagc
    10981 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
    11041 ggtcttgagg ggttttttgc tgaaaggagg aactactaga gcgatcccgc gaaattaata
    11101 cgactcacta taggggaatt gtgagcggat aacaattccc cactagaaat aattttgttt
    11161 aactttaaga aggagatata catatggctc gtacaacacc catcgcacgc taccgtaaca
    11221 tcggtatcag tgcgcacatc gacgccggta aaaccactac taccgaacgt attctgttct
    11281 acaccggtgt aaaccataaa atcggtgaag ttcatgacgg cgctgcaacc atggactgga
    11341 tggagcagga gcaggaacgt ggtattacca tcacttccgc tgcgactact gcattctggt
    11401 ctggtatggc taagcagtat gagccgcatc gcatcaacat catcgacacc ccggggcacg
    11461 ttgacttcac aatcgaagta gaacgttcca tgcgtgttct cgatggtgcg gtaatggttt
    11521 actgcgcagt tggtggtgtt cagccgcagt ctgaaaccgt atggcgtcag gcaaacaaat
    11581 ataaagttcc gcgcattgcg ttcgttaaca aaatggaccg catgggtgcg aacttcctga
    11641 aagttgttaa ccagatcaaa acccgtctgg gcgcgaaccc ggttccgctg cacctggcga
    11701 ttggtgctga agaacatttc accggtgttg ttgacctggt gaaaatgaaa gctatcaact
    11761 ggaacgacgc tgaccagggc gtaaccttcg aatacgaaga tatcccggca gacatggttg
    11821 aactggctaa cgaatggcac cagaacctga tcgaatccgc agctgaagct tctgaagagc
    11881 tgatggaaaa atacctgggt ggtgaagaac tgactgaagc agaaatcaaa ggtgctctgc
    11941 gtcagcgcgt tctgaacaac gaaatcatcc tggtaacctg tggttctgcg ttcaagaaca
    12001 aaggtgttca ggcgatgctg gatgcggtaa ttgattacct gccatccccg gttgacgtac
    12061 ctgcgatcaa cggtatcctg gacgacggta aagacactcc ggctgaacgt cacgcaagtg
    12121 atgacgagcc gttctctgca ctggcgttca aaatcgctac cgacccgttt gttggtaacc
    12181 tgaccttctt ccgtgtttac tccggtgtgg ttaactctgg tgataccgta ctgaactccg
    12241 tgaaagctgc acgtgagcgt ttcggtcgta tcgttcagat gcacgctaac aaacgtgaag
    12301 agatcaaaga agttcgcgcg ggcgacatcg ctgctgctat cggtctgaaa gacgtaacca
    12361 ctggtgacac cctgtgtgac ccggatgcgc cgatcattct ggaacgtatg gagttccctg
    12421 agccggtaat ctccatcgca gttgaaccga aaaccaaagc tgaccaggaa aaaatgggtc
    12481 tggctctggg ccgtctggct aaagaagacc cgtctttccg tgtatggact gacgaagaat
    12541 ctaaccagac catcatcgcg ggtatgggcg aactgcacct cgacatcatc gttgaccgta
    12601 tgaagcgtga gttcaacgtt gaagcgaacg taggtaaacc gcaggttgct taccgtgaaa
    12661 ctatccgcca gaaagttacc gatgttgaag gtaaacacgc gaaacagtct ggtggtcgtg
    12721 gtcagtatgg tcatgttgtt atcgacatgt acccgctgga gccgggttca aacccgaaag
    12781 gctacgagtt catcaacgac attaaaggtg gtgtaatccc tggcgaatac atcccggccg
    12841 ttgataaagg tatccaggaa cagctgaaag caggtccgct ggcaggctac ccggtagtag
    12901 acatgggtat tcgtctgcac ttcggttctt accatgacgt tgactcctct gaactggcgt
    12961 ttaaactggc tgcttctatc gcctttaaag aaggctttaa gaaagcgaaa ccagttctgc
    13021 ttgagccgat catgaaggtt gaagtagaaa ctccggaaga gaacaccggt gacgttatcg
    13081 gtgacttgag ccgtcgtcgt ggtatgctca aaggtcagga atctgaagtt actggcgtta
    13141 agatccacgc tgaagtaccg ctgtctgaaa tgttcggata cgcaactcag ctgcgttctc
    13201 tgaccaaagg tcgtgcatca tacactatgg agttcctgaa gtatgatgaa gcgccgagta
    13261 acgttgctca ggccgtaatt gaagcccgtg gtaaacacca ccaccaccac cactaattat
    13321 ctgttagttt ttttctacta gactgctaac aaagcccgaa aggaagctga gttggctgct
    13381 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
    13441 tttttgctga aaggaggaac tactagagcg atcccgcgaa attaatacga ctcactatag
    13501 gggaattgtg agcggataac aattccccac tagaaataat tttgtttaac tttaagaagg
    13561 agatatacat atgcaccacc accaccacca cacagatgta acgattaaaa cgctggccgc
    13621 agagcgacag acctccgtgg aacgcctggt acagcaattt gctgatgcag gtatccggaa
    13681 gtctgctgac gactctgtgt ctgcacaaga gaaacagact ttgattgacc acctgaatca
    13741 gaaaaactca ggcccggaca aattgacgct gcaacgtaaa acacgcagca cccttaacat
    13801 tcctggtacc ggtggaaaaa gcaaatcggt acaaatcgaa gtccgcaaga aacgcacctt
    13861 tgtgaaacgc gatccgcaag aggctgaacg ccttgcagcg gaagagcaag cgcagcgtga
    13921 agcggaagag caagcccgtc gtgaggcaga agaatcggct aaacgcgagg cgcaacaaaa
    13981 agctgaacgt gaggccgcag aacaagctaa gcgtgaagct gctgaacaag cgaaacgtga
    14041 agctgcggaa aaagacaaag tgagcaatca acaagacgat atgactaaaa acgcccaggc
    14101 tgaaaaagcc cgccgtgagc aggaagctgc ggagctcaag cgtaaagctg aagaagaagc
    14161 gcgtcgtaaa ctcgaagaag aagcacgtcg cgttgctgaa gaagcacgtc gtatggcgga
    14221 agaaaacaaa tggactgata acgcggaacc gactgaagat tccagcgatt atcacgtcac
    14281 tacttctcaa catgctcgcc aggcagaaga cgaaagcgat cgtgaagtcg aaggcggccg
    14341 tggccgtggt cgtaacgcga aagcagcgcg tccgaagaaa ggcaacaaac acgctgaatc
    14401 aaaagctgat cgtgaagaag cacgcgcagc agtacgtggc ggtaaaggcg gaaaacgtaa
    14461 aggttcttcg ctgcaacaag gcttccagaa gcctgctcag gccgttaacc gtgacgttgt
    14521 gatcggcgaa actatcaccg ttggcgaact ggcgaacaag atggcggtta aaggctctca
    14581 ggtcatcaaa gcgatgatga aactgggcgc aatggcaacc atcaaccagg ttatcgatca
    14641 ggaaaccgca cagctggttg ctgaagagat gggccataaa gttatcctgc gtcgtgaaaa
    14701 cgagctggaa gaggcggtaa tgagcgaccg tgacacgggt gctgcggctg aaccgcgcgc
    14761 gccggttgtg accatcatgg gtcacgttga ccacggtaaa acctctctgc tggactacat
    14821 tcgttcaacg aaagtggcct ctggcgaagc gggcggcatt acccagcaca ttggtgcata
    14881 ccacgttgaa actgaaaacg gcatgatcac cttcctggac accccggggc acgccgcgtt
    14941 tacttcaatg cgtgctcgtg gtgcgcaggc aacggacatc gtagtcctgg ttgttgctgc
    15001 cgacgacggt gtgatgccgc agaccatcga agcaatccag cacgcgaaag cggcgcaggt
    15061 accggtggtg gttgcagtga acaagatcga taaaccagaa gctgatccgg atcgcgttaa
    15121 gaacgaactc tcccagtacg gcatcctgcc ggaagagtgg ggcggtgaaa gccagttcgt
    15181 acacgtatct gcgaaagcgg gtaccggtat cgatgaactg ctggacgcta tcctgctgca
    15241 agcggaagtt ctggagctga aagcggtacg taaaggtatg gcgagcggtg cggttatcga
    15301 atccttcctc gataaaggtc gtggtccggt tgctaccgtt ctggtacgtg aaggtactct
    15361 gcacaagggc gatatcgttc tgtgtggctt cgaatacggt cgtgttcgtg cgatgcgtaa
    15421 cgaactgggt caggaagtgc tggaagcggg tccgtccatt ccggtggaaa tcctcggcct
    15481 gtccggcgta ccggctgcgg gtgatgaagt taccgttgta cgtgacgaga agaaagcgcg
    15541 tgaagttgca ctctatcgtc agggtaagtt ccgcgaagtt aaactggcgc gtcagcagaa
    15601 atctaaactc gagaacatgt tcgccaacat gaccgaaggc gaagttcacg aagtgaatat
    15661 cgtcctgaag gcagacgtac agggttctgt cgaagcgatc tccgactcct tgctgaaact
    15721 gtctactgac gaagttaaag tgaagatcat cggttctggc gtaggtggta tcaccgaaac
    15781 cgacgccacc ctggctgcgg cgtccaacgc catcctggtt ggctttaacg tacgtgctga
    15841 tgcctctgca cgtaaagtga ttgaagcgga aagcctggat ctgcgttact actccgtcat
    15901 ctataacctg attgacgaag tgaaagcggc gatgagcggt atgctgtctc cggaactgaa
    15961 acagcagatt atcggtctgg cggaagttcg tgacgtgttc aaatcgccga agtttggtgc
    16021 catcgcaggc tgtatggtta ccgaaggtgt ggttaaacgt cacaacccga tccgcgttct
    16081 gcgtgacaac gtggttatct acgaaggcga gctggagtcc ctgcgccgct tcaaagatga
    16141 cgttaacgaa gtccgtaacg gtatggaatg tggtatcggc gttaagaact acaacgacgt
    16201 ccgcactggc gatgtgatcg aagtattcga aatcatcgag atccaacgta ccattgctta
    16261 attatctgtt agtttttttc tactagactg ctaacaaagc ccgaaaggaa gctgagttgg
    16321 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
    16381 ggggtttttt gctgaaagga ggaactacta gtagcggccg ctgcagtccg gattggcgaa
    16441 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
    16501 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
    16561 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
    16621 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
    16681 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
    16741 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
    16801 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
    16861 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
    16921 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
    16981 tgaattaatt cttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
    17041 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
    17101 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
    17161 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
    17221 tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt
    17281 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
    17341 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa
    17401 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
    17461 gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt
    17521 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
    17581 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
    17641 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct
    17701 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
    17761 tttaatcgcg gcctagagca agacgtttcc cgttgaatat ggctcataac accccttgta
    17821 ttactgttta tgtaagcaga cagttttatt gttcatgacc aaaatccctt aacgtgagtt
    17881 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
    17941 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
    18001 tttgccggat caagagctac caactctttt tcaaggtaac tggcttcagc agagcgcaga
    18061 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    18121 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    18181 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    18241 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    18301 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    18361 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    18421 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    18481 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    18541 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    18601 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    18661 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
    18721 ttacgcatct gtgcggtatt tcacaccgca atggtgcact ctcagtacaa tctgctctga
    18781 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
    18841 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
    18901 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
    18961 tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca
    19021 cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc
    19081 tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc
    19141 tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg
    19201 ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa
    19261 caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc
    19321 ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc
    19381 cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa
    19441 ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac
    19501 gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc
    19561 cgggtcctca acgacaggag cacgatcatg cgcacccgtg gggccgccat gccggcgata
    19621 atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg
    19681 gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag
    19741 cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata
    19801 aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg
    19861 actgggttga aggctctcaa gggcatcggt cgagatcccg gtgcctaatg agtgagctaa
    19921 cttacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
    19981 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt
    20041 ggtttttctt ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg
    20101 agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat
    20161 ggtggttaac ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga
    20221 gatatccgca ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat
    20281 ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt
    20341 ttgttgaaaa ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg
    20401 attgcgagtg agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa
    20461 tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag
    20521 tcgcgtaccg tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc
    20581 aagaaataac gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc
    20641 cagcggatag ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc
    20701 tttacaggct tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg
    20761 atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga
    20821 ggtggcaacg ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg
    20881 aatgtaattc agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg
    20941 gctggcctgg ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac
    21001 atcgtataac gttactggtt tcacattcac caccctgaat tgactctctt ccgggcgcta
    21061 tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg tccgggatct cgacgctctc
    21121 ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg
    21181 ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc
    21241 ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt
    21301 ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc
    21361 cggccacgat gcgtccggcg tagaggatcg agatctgaat tcgcggccgc ttctagagcg
    21421 atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac aattccccac
    21481 tagaaataat tttgtttaac tttaagaagg agatatacat atgcgtacag aatattgtgg
    21541 acagctccgt ttgtcccacg tggggcagca ggtgactctg tgtggttggg tcaaccgtcg
    21601 tcgtgatctt ggtagcctga tcttcatcga tatgcgcgac cgcgaaggta tcgtgcaggt
    21661 atttttcgat ccggatcgtg cggacgcgtt aaagctggcc tctgaactgc gtaatgagtt
    21721 ctgcattcag gtcacgggca ccgtacgtgc gcgtgacgaa aaaaatatta accgcgatat
    21781 ggcgaccggc gaaatcgaag tgctggcgtc ctcgctgact atcatcaacc gcgcagatgt
    21841 tctgccgctt gactctaacc acgtcaacac cgaagaagcg cgtctgaaat accgctacct
    21901 cgacctgcgt cgtccggaaa tggctcagcg cctgaaaacc cgcgctaaaa tcaccagcct
    21961 ggtgcgccgt tttatggatg accacggctt cctcgacatc gaaactccga tgctgaccaa
    22021 agccacgccg gaaggcgcgc gtgactacct ggtgccttct cgtgtgcaca aaggtaagtt
    22081 ctacgcactg ccgcaatccc cgcagttgtt caaacagctg ctgatgatgt ccggttttga
    22141 ccgttactat cagatcgtta aatgcttccg tgacgaagac ctgcgtgctg accgtcagcc
    22201 tgagtttact cagatcgatg tggaaacttc tttcatgacc gcgccgcaag tgcgtgaagt
    22261 gatggaagcg ctggtgcgtc atctgtggct ggaagtgaag ggtgtggatc tgggcgattt
    22321 cccggtaatg acctttgcgg aagcagaacg ccgttatggt tctgataaac cggatctgcg
    22381 taacccgatg gaactgactg acgttgctga tctgctgaaa tctgttgagt ttgctgtatt
    22441 tgcaggtccg gcgaacgatc cgaaaggtcg cgtagcggct ctgcgcgttc cgggcggcgc
    22501 atcgctgacc cgtaagcaga tcgacgaata cggtaacttc gttaaaatct acggcgcgaa
    22561 aggtctggct tacatcaaag ttaacgaacg cgcgaaaggt ctggaaggta tcaacagccc
    22621 ggtagcgaag ttccttaatg cagaaatcat cgaagacatc ctggatcgta ctgccgcgca
    22681 agatggcgat atgattttct tcggtgccga caacaagaaa attgttgccg acgcgatggg
    22741 tgcactgcgc ctgaaagtgg gtaaagacct tggtctgacc gacgaaagca aatgggcacc
    22801 gctgtgggtt atcgacttcc cgatgtttga agacgacggt gaaggcggcc tgacggcaat
    22861 gcaccatccg ttcacctcac cgaaagatat gacggcggca gaactgaaag ctgcaccgga
    22921 aaatgcggtg gcgaacgctt acgatatggt catcaatggt tacgaagtgg gcggtggttc
    22981 agtacgtatc cataatggtg atatgcagca gacggtgttt ggtattctgg gtatcaacga
    23041 agaggaacag cgcgagaagt tcggcttcct gctcgacgct ctgaaatacg gtactccgcc
    23101 gcacgcaggt ctggcattcg gtcttgaccg tctgaccatg ctgctgaccg gcaccgacaa
    23161 tatccgtgac gttatcgcct tcccgaaaac cacggcggca gcgtgtctga tgactgaagc
    23221 accgagcttt gctaacccga ctgcactggc tgagctgagc attcaggttg tgaagaaggc
    23281 tgagaataac caccaccacc accaccacta attatctgtt agtttttttc tactagactg
    23341 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctga
//
