LOCUS       sequence-31609-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CAPN13
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..55
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             65..82
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             577..594
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(647..665)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      661..708
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(832..851)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tttaacttta agaaggagan ataccatggg cagcagccat catcatcatc atcacagcag
       61 cggcctggtt ccgcgtggta gtgacattga tgccacccag cttcagggcc ttctcaacca
      121 ggagcttcta acaggacctc caggggacat gttctcctta gatgagtgcc gcagcttggt
      181 ggctctgatg gaactgaaag tgaatgggcg gctagaccaa gaggagtttg cgcgactgtg
      241 gaagcgcctt gttcactacc agcatgtttt ccagaaggtt cagacaagcc ctggagtcct
      301 cctgagctcg gacttgtgga aggccataga gaatacagac ttcctcagag ggatcttcat
      361 cagccgtgag ctgctgcatc tggtgaccct caggtacagc gacagcgtcg gcagggtcag
      421 cttccccagc ctggtctgct tcctgatgcg gcttgaagcc atggcaaaga ccttccgcaa
      481 cctctctaag gatggaaaag gactctacct gacagaaatg gagtggatga gcctggtcat
      541 gtacaactga tgacgaagct tgcggccgca ctcgagcacc accaccacca ccactgagat
      601 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
      661 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
      721 actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
      781 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
      841 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
      901 ggctcccttt agggttccga tttagtgctt tacggcacct cga
//
