LOCUS       sequence-316090-        6587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0411:Dendra
FEATURES             Location/Qualifiers
     source          1..6587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2531..2554
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2811..3611
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3736..4324
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4225..4244
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tgctgcacgg ccgtgccagc ctgcgtcccg acgacatcgc
      121 gttcaccttc accgactacg acggtgatcc gtcgggcgtc gcgcagagcc tgacgtggtc
      181 cgtgctggcg cgtcgaacga tcaacgtggc cagagagatc cggcgccacg gcgccgtggg
      241 ggaccgggct ctgatactcg cgccgcaggg actcgaatac atcctcgcgt tcctgggatc
      301 gatgcaggcc ggactcatcg cggtaccgtt gccgttgccg catcgggggt cgggacacga
      361 ccgggtgagt gcggtgttcg ccgacaccga gccgacggtc gtgctcacga cgtcggcagc
      421 cgccaccgac gtcgccgaat tcgtcgcgtc gtcgcgcacc gatccggcga tcatcgagat
      481 cgattcgctt gctctggatt ccgactactc cggcgcggaa accgatctcg gtccggtcgg
      541 gctgtccgac atcgcctacc tgcagtacag ctccggatcg acgcggctgc cgaccggggt
      601 catgatctcg caccgcaacc tcaccgcgaa cttcgaccag ttgatgcgga gtttcttcgc
      661 caggtcgccg cttccgcagg atgcgacgct cgtgtcgtgg ctgcccttct atcacgacat
      721 gggcctggtg ctgggggtgt gcgcgccgat cctgtgcggg cgccacgcac agctcacgag
      781 cccggtggcg ttcctggaac gtccggcccg gtgggtgcgg gcgctcgccg agaatccaca
      841 cgcctggtcg tcggcaccca acttcgcgtt cgacctcgcg gcacgcagga ccagcgacga
      901 ggatctggcc gggctggacc tcggcggggt ggtgggcatc atcagcggcg ccgaacgcgt
      961 cgaatcggcc acgctgcgcc gcttcgtgga ccgcttcgcg cacttcaact ttcgtgacca
     1021 catgatgcgt cccgcgtacg ggctggcgga ggccacggtg ttcgtggcct cgggcatctg
     1081 ggacgaggat gcgccggccg ggtacttcga cgccgaagcg ctgagcgcgg gccgggtcag
     1141 tcccagcgcg ccgcgcaagg gcaccgcact ggtgagatac caggtgccgc agtcaccttc
     1201 gctgcgaatc gtcgacgtcg acacgcgccg cgagaccccg ccgggtgtgg tgggagagat
     1261 ctgggtgcac ggcgacaacg tcgcctccgg gtactggggc aaaccggcgg acgagagcac
     1321 gttcggcgca acacttctcg acccgtcgcc gggtacgccc gatggcccgt ggctgcgcac
     1381 gggtgacctg gggttcgtcc atgccggcga actgtacgtc gtcggacgca tcaaggacgt
     1441 cctgatcatc cggggccgca accactatcc cgaggatatc gaggccacgg tgcaggccat
     1501 cacccacggc agggtggcgg ccatctcggt tccggtgaac agcaccgagg agttggtgac
     1561 cgtcatcgaa ctcaagaagc cggccgacct cgacgccgag gccatccgtc ggttcggcga
     1621 ggtcaagagc aacgtcaccg ccgcggtggc gaacgcgcac ggcctgagcg tggcggatct
     1681 ggttctcgtg ccgtcgggtt cgctcccgac caccacgagc ggcaagatcc ggcgtgcggc
     1741 ctgtgcagag cagtaccggc agaagcagtt cgtcaggttg gacggcaagc ttggtgcggc
     1801 cgcgggcaac acgcctggca tcaatctgat caaggaggac atgcgtgtca aggtgcatat
     1861 ggagggcaac gtgaacggtc acgccttcgt gatcgagggg gagggtaagg gtaagcctta
     1921 cgaagggacc cagaccgcca acctcaccgt caaggagggc gcccctctcc cattctcgta
     1981 cgatatcctc actaccgcgg tgcattacgg gaaccgcgtg ttcaccaagt accccgagga
     2041 tatccctgac tacttcaagc agagcttccc ggagggatac tcctgggagc ggacgatgac
     2101 cttcgaagac aagggtatct gtacgatccg cagcgacatc tcgctggaag gggactgttt
     2161 cttccagaac gtccgtttca aggggaccaa ctttccgccg aacggcccgg tcatgcagaa
     2221 gaagaccctc aagtgggagc cgagcacgga gaagctgcac gttcgggacg gcctcctggt
     2281 cggcaacatc aacatggcgc tgctgctgga aggcggcggc cactacctat gcgacttcaa
     2341 gaccacgtac aaagcgaaga aggtcgtcca gctgcccgac gcgcacttcg tggaccaccg
     2401 catcgaaatc ctggggaacg attcggacta caacaaggtc aagttgtacg agcacgctgt
     2461 ggcacgctat agcccgctcc cgtcgcaggt ctggttcgaa ggcggcggcg gggagaacct
     2521 gtatttccag gactacaagg acgacgacga caagtgattt aaataacccc ttggggcctc
     2581 taaacgggtc ttgaggggtt ttttgttaac tagcgtacga tcgaattcct gcagctcacg
     2641 gtaactgatg ccgtatttgc agtaccagcg tacggcccac agaatgatgt cacgctgaaa
     2701 atgccggcct ttgaatgggt tcatgtgcag ctccatcagc aaaaggggat gataagttta
     2761 tcaccaccga ctatttgcaa cagtgccgtt gatcgtgcta tgatcgactg atgtcatcag
     2821 cggtggagtg caatgtcgtg caatacgaat ggcgaaaagc cgagctcatc ggtcagcttc
     2881 tcaaccttgg ggttaccccc ggcggtgtgc tgctggtcca cagctccttc cgtagcgtcc
     2941 ggcccctcga agatgggcca cttggactga tcgaggccct gcgtgctacg ctgggtccgg
     3001 gagggacgct cgtcatgccc tcgtggtcag gtctggacga cgagccgttc gatcctgcca
     3061 cgtcgcccgt tacaccggac cttggagttg tctctgacac attctggcgc ctgccaaatg
     3121 taaagcgcag cgcccatcca tttgcctttg cggcagcggg gccacaggca gagcagatca
     3181 tctctgatcc attgcccctg ccaccttact cgcctgcaag cccggtcgcc cgtgtccatg
     3241 aactcgatgg gcaggtactt ctcctcggcg tgggacacga tgccaacacg acgctgcatc
     3301 ttgccgagtt gatggcaaag gttccctatg gggtgccgag acactgcacc attcttcagg
     3361 atggcaagtt ggtacgcgtc gattatctcg agaatgacca ctgctgtgag cgctttgcct
     3421 tggcggacag gtggctcaag gagaagagcc ttcagaagga aggtccagtc ggtcatgcct
     3481 ttgctcggtt gatccgctcc cgcgacattg tggcgacagc cctgggtcaa ctgggccgag
     3541 atccgttgat cttcctgcat ccgccagagg cgggatgcga agaatgcgat gccgctcgcc
     3601 agtcgattgg ctgagctcat gagcggagaa cgagatgacg ttggaggggc aaggtcgcgc
     3661 tgattgctgg ggcaacacgg gggatccact agttccatga gcgtcagacc ccgtagaaaa
     3721 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3781 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3841 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     3901 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3961 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4021 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4081 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4141 cacgcttccc gaggggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4201 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4261 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4321 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4381 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     4441 agctgatacc gctcgccgca gccgaacgac cgagcgcaac gcgtgcggcc gcggtacccg
     4501 gggatcctct agagtcgacc accaagggca ccatctctgc ttgggccacc ccgttggccg
     4561 cagccagctc gctgagagcc gtgaacgaca gggcgaacgc cagcccgccg acggcgaggg
     4621 ttccgaccgc tgcaactccc ggtgcaacct tgtcccggtc tattctcttc actgcaccag
     4681 ctccaatctg gtgtgaatgc ccctcgtctg ttcgcgcagg cggggggctc tattcgtttg
     4741 tcagcatcga aagtagccag atcagggatg cgttgcaacc gcgtatgccc aggtcagaag
     4801 agtcgcacaa gagttgcaga cccctggaaa gaaaaatggc cagagggcga aaacaccctc
     4861 tgaccagcgg agcgggcgac gggaatcgaa cccgcgtagc tagtttggaa gaatgggtgt
     4921 ctgccgacca catatgggcc ggtcaagata ggtttttacc ccctctcggc tgcatcctct
     4981 aagtggaaag aaattgcagg tcgtagaagc gcgttgaagc ctgagagttg cacaggagtt
     5041 gcaacccggt agccttgttc acgacgagag gagacctagt tggcacgtcg cggatgggga
     5101 tcgctgaaga ctcagcgcag cgggaggatc caagcctcat acgtcaaccc gcaggacggt
     5161 gtgaggtact acgcgctgca gacctacgac aacaagatgg acgccgaagc ctggctcgcg
     5221 ggcgagaagc ggctcatcga gatggagacc tggacccctc cacaggaccg ggcgaagaag
     5281 gcagccgcca gcgccatcac gctggaggag tacacccgga agtggctcgt ggagcgcgac
     5341 ctcgcagacg gcaccaggga tctgtacagc gggcacgcgg agcgccgcat ctacccggtg
     5401 ctaggtgaag tggcggtcac agagatgacg ccagctctgg tgcgtgcgtg gtgggccggg
     5461 atgggtagga agcacccgac tgcccgccgg catgcctaca acgtcctccg ggcggtgatg
     5521 aacacagcgg tcgaggacaa gctgatcgca gagaacccgt gccggatcga gcagaaggca
     5581 gccgatgagc gcgacgtaga ggcgctgacg cctgaggagc tggacatcgt cgccgctgag
     5641 atcttcgagc actaccggat cgcggcatac atcctggcgt ggacgagcct ccggttcgga
     5701 gagctgatcg agcttcgccg caaggacatc gtggacgacg gcatgacgat gaagctccgg
     5761 gtgcgccgtg gcgcttcccg cgtggggaac aagatcgtcg ttggcaacgc caagaccgtc
     5821 cggtcgaagc gtcctgtgac ggttccgcct cacgtcgcgg agatgatccg agcgcacatg
     5881 aaggaccgta cgaagatgaa caagggcccc gaggcattcc tggtgaccac gacgcagggc
     5941 aaccggctgt cgaagtccgc gttcaccaag tcgctgaagc gtggctacgc caagatcggt
     6001 cggccggaac tccgcatcca cgacctccgc gctgtcggcg ctacgttcgc cgctcaggca
     6061 ggtgcgacga ccaaggagct gatggcccgt ctcggtcaca cgactcctag gatggcgatg
     6121 aagtaccaga tggcgtctga ggcccgcgac gaggctatcg ctgaggcgat gtccaagctg
     6181 gccaagacct cctgaaacgc aaaaagcccc cctcccaagg acactgagtc ctaaagaggg
     6241 gggtttcttg tcagtacgcg aagaaccacg cctggccgcg agcgccagca ccgccgctct
     6301 gtgcggagac ctgggcacca gccccgccgc cgccaggagc attgccgttc ccgccagaaa
     6361 tctagacggt gaccacaacg cgcccgcttt gatcggggac gtcgaaatat tggatcgtcg
     6421 gcaccgtcac ggccgtggga ggcggcacga tccgcgacgt gatgatcggc cgcatcccca
     6481 cggtgctgcg cagtgagctc tacgccatcc cggcgttgat ctgtgcgttc gcacgcacag
     6541 gcccggtgtg agaagggtct ctgacgagcg ggagaactcc ctatcag
//
