LOCUS       sequence-316092-        6746 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 expression of C-term fluorescent fusion in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0709:Dendra
FEATURES             Location/Qualifiers
     source          1..6746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2690..2713
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2970..3770
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3895..4483
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4384..4403
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
       61 tagggagaag gaggccatta tggctcgtgc ggtcggtatc gacctcggga ccaccaactc
      121 cgtcgtggcg gttctggaag gcggcgatcc agtcgtcgtc gcgaactccg agggctctcg
      181 gaccacccct tcggtcgtcg cgttcgcgcg caacggcgag gtgctggtcg gtcagcccgc
      241 caagaaccag gcggtgacca acgtcgaccg caccatccgt tcggtcaagc gccacgtcgg
      301 cacggactgg aacatcgaga tcgacgacaa gaagtacacg ccgcaggaga tcagcgcgcg
      361 tgtgctcatg aagctcaagc gtgacgccga gagctacctc ggtgaggaca tcaccgacgc
      421 cgtcatcacg gtcccggcgt acttcaacga cgcccagcgt caggccacca aggaagccgg
      481 ccagatcgcc ggcctcaacg tgctccgcat cgtcaacgag cccaccgcgg ccgcgctggc
      541 gtacggcctg gacaagggcg agaaggaaca gaccatcctg gtcttcgacc tcggtggcgg
      601 cacgttcgac gtctcgctgc tggagatcgg cgacggtgtc gtcgaggtcc gcgcgacctc
      661 cggtgacaac cacctcggtg gcgacgactg ggacgaccgg atcgtcacgt ggctggtcga
      721 caagttcaag ggcagctcgg gcatcgacct gaccaaggac aagatggcca tgcagcgcct
      781 gcgtgaggcg gccgagaagg ccaagatcga actctcgagc tcgcagagca cgtcgatcaa
      841 cctgccgtac atcacggtcg acgccgacaa gaacccactg ttcctcgacg agcagctgac
      901 ccgcgcggag ttccagcgca tcacccagga tctgctcgac cgcacccggc agccgttcca
      961 gcaggtcatc aaggacgccg gcatctcggt gtccgacatc gaccacgtgg tgctggtggg
     1021 cggttcgacc cgtatgcccg cggtgaccga cctggtcaag gaactgaccg gcggcaagga
     1081 gcccaacaag ggcgtcaacc ccgacgaggt tgtcgcggtg ggtgctgcgc tgcaggccgg
     1141 tgtgctcaag ggcgaggtga aagacgttct gctgcttgac gtcaccccgc tatccctcgg
     1201 tatcgagacc aagggtggcg tgatgaccaa gctgatcgag cgcaacacca cgatcccgac
     1261 caagcggtcg gagaccttca ccaccgccga cgacaaccag ccgtcggtgc agatccaggt
     1321 ctaccagggt gagcgcgaga tcgcctcgca caacaagctg ctcggcagct tcgagctgac
     1381 cggtatcccg ccggccccgc gtggcgtgcc ccagatcgag gtcaccttcg acatcgacgc
     1441 caacggcatc gtccacgtca ccgccaagga caagggcacc ggcaaggaga acacgatcaa
     1501 gatccaggaa ggctccggcc tttccaagga agagatcgac cggatgatca aggacgccga
     1561 ggcgcacgcc gaggaggacc gcaagcgtcg cgaagaggcc gacgtgcgca accaggccga
     1621 gtcgctggtc taccagacgg agaagttcgt cgctgaacag aggggcgcag cgtccgacgg
     1681 tggcggttcg aaggtgcccg aggagacgct ggccaaggtc gattcggcga tcgccgacgc
     1741 caagaaggcg ctcgaaggca ccgacatctc ggccatcaag tccgctatgg agaagctcgg
     1801 cgtggagagc caggccctgg gccaggcgat ctacgaggcc acccaggccg aacagcccgc
     1861 gggcggctcg gacaacgggg ccccgggtga cgacaacgtg gtcgacgccg aggttgtcga
     1921 cgatgacgct ggcaaggaga acaagaagct tggtgcggcc gcgggcaaca cgcctggcat
     1981 caatctgatc aaggaggaca tgcgtgtcaa ggtgcatatg gagggcaacg tgaacggtca
     2041 cgccttcgtg atcgaggggg agggtaaggg taagccttac gaagggaccc agaccgccaa
     2101 cctcaccgtc aaggagggcg cccctctccc attctcgtac gatatcctca ctaccgcggt
     2161 gcattacggg aaccgcgtgt tcaccaagta ccccgaggat atccctgact acttcaagca
     2221 gagcttcccg gagggatact cctgggagcg gacgatgacc ttcgaagaca agggtatctg
     2281 tacgatccgc agcgacatct cgctggaagg ggactgtttc ttccagaacg tccgtttcaa
     2341 ggggaccaac tttccgccga acggcccggt catgcagaag aagaccctca agtgggagcc
     2401 gagcacggag aagctgcacg ttcgggacgg cctcctggtc ggcaacatca acatggcgct
     2461 gctgctggaa ggcggcggcc actacctatg cgacttcaag accacgtaca aagcgaagaa
     2521 ggtcgtccag ctgcccgacg cgcacttcgt ggaccaccgc atcgaaatcc tggggaacga
     2581 ttcggactac aacaaggtca agttgtacga gcacgctgtg gcacgctata gcccgctccc
     2641 gtcgcaggtc tggttcgaag gcggcggcgg ggagaacctg tatttccagg actacaagga
     2701 cgacgacgac aagtgattta aataacccct tggggcctct aaacgggtct tgaggggttt
     2761 tttgttaact agcgtacgat cgaattcctg cagctcacgg taactgatgc cgtatttgca
     2821 gtaccagcgt acggcccaca gaatgatgtc acgctgaaaa tgccggcctt tgaatgggtt
     2881 catgtgcagc tccatcagca aaaggggatg ataagtttat caccaccgac tatttgcaac
     2941 agtgccgttg atcgtgctat gatcgactga tgtcatcagc ggtggagtgc aatgtcgtgc
     3001 aatacgaatg gcgaaaagcc gagctcatcg gtcagcttct caaccttggg gttacccccg
     3061 gcggtgtgct gctggtccac agctccttcc gtagcgtccg gcccctcgaa gatgggccac
     3121 ttggactgat cgaggccctg cgtgctacgc tgggtccggg agggacgctc gtcatgccct
     3181 cgtggtcagg tctggacgac gagccgttcg atcctgccac gtcgcccgtt acaccggacc
     3241 ttggagttgt ctctgacaca ttctggcgcc tgccaaatgt aaagcgcagc gcccatccat
     3301 ttgcctttgc ggcagcgggg ccacaggcag agcagatcat ctctgatcca ttgcccctgc
     3361 caccttactc gcctgcaagc ccggtcgccc gtgtccatga actcgatggg caggtacttc
     3421 tcctcggcgt gggacacgat gccaacacga cgctgcatct tgccgagttg atggcaaagg
     3481 ttccctatgg ggtgccgaga cactgcacca ttcttcagga tggcaagttg gtacgcgtcg
     3541 attatctcga gaatgaccac tgctgtgagc gctttgcctt ggcggacagg tggctcaagg
     3601 agaagagcct tcagaaggaa ggtccagtcg gtcatgcctt tgctcggttg atccgctccc
     3661 gcgacattgt ggcgacagcc ctgggtcaac tgggccgaga tccgttgatc ttcctgcatc
     3721 cgccagaggc gggatgcgaa gaatgcgatg ccgctcgcca gtcgattggc tgagctcatg
     3781 agcggagaac gagatgacgt tggaggggca aggtcgcgct gattgctggg gcaacacggg
     3841 ggatccacta gttccatgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3901 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3961 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4021 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     4081 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4141 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4201 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4261 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aggggagaaa
     4321 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4381 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4441 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4501 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     4561 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     4621 ccgaacgacc gagcgcaacg cgtgcggccg cggtacccgg ggatcctcta gagtcgacca
     4681 ccaagggcac catctctgct tgggccaccc cgttggccgc agccagctcg ctgagagccg
     4741 tgaacgacag ggcgaacgcc agcccgccga cggcgagggt tccgaccgct gcaactcccg
     4801 gtgcaacctt gtcccggtct attctcttca ctgcaccagc tccaatctgg tgtgaatgcc
     4861 cctcgtctgt tcgcgcaggc ggggggctct attcgtttgt cagcatcgaa agtagccaga
     4921 tcagggatgc gttgcaaccg cgtatgccca ggtcagaaga gtcgcacaag agttgcagac
     4981 ccctggaaag aaaaatggcc agagggcgaa aacaccctct gaccagcgga gcgggcgacg
     5041 ggaatcgaac ccgcgtagct agtttggaag aatgggtgtc tgccgaccac atatgggccg
     5101 gtcaagatag gtttttaccc cctctcggct gcatcctcta agtggaaaga aattgcaggt
     5161 cgtagaagcg cgttgaagcc tgagagttgc acaggagttg caacccggta gccttgttca
     5221 cgacgagagg agacctagtt ggcacgtcgc ggatggggat cgctgaagac tcagcgcagc
     5281 gggaggatcc aagcctcata cgtcaacccg caggacggtg tgaggtacta cgcgctgcag
     5341 acctacgaca acaagatgga cgccgaagcc tggctcgcgg gcgagaagcg gctcatcgag
     5401 atggagacct ggacccctcc acaggaccgg gcgaagaagg cagccgccag cgccatcacg
     5461 ctggaggagt acacccggaa gtggctcgtg gagcgcgacc tcgcagacgg caccagggat
     5521 ctgtacagcg ggcacgcgga gcgccgcatc tacccggtgc taggtgaagt ggcggtcaca
     5581 gagatgacgc cagctctggt gcgtgcgtgg tgggccggga tgggtaggaa gcacccgact
     5641 gcccgccggc atgcctacaa cgtcctccgg gcggtgatga acacagcggt cgaggacaag
     5701 ctgatcgcag agaacccgtg ccggatcgag cagaaggcag ccgatgagcg cgacgtagag
     5761 gcgctgacgc ctgaggagct ggacatcgtc gccgctgaga tcttcgagca ctaccggatc
     5821 gcggcataca tcctggcgtg gacgagcctc cggttcggag agctgatcga gcttcgccgc
     5881 aaggacatcg tggacgacgg catgacgatg aagctccggg tgcgccgtgg cgcttcccgc
     5941 gtggggaaca agatcgtcgt tggcaacgcc aagaccgtcc ggtcgaagcg tcctgtgacg
     6001 gttccgcctc acgtcgcgga gatgatccga gcgcacatga aggaccgtac gaagatgaac
     6061 aagggccccg aggcattcct ggtgaccacg acgcagggca accggctgtc gaagtccgcg
     6121 ttcaccaagt cgctgaagcg tggctacgcc aagatcggtc ggccggaact ccgcatccac
     6181 gacctccgcg ctgtcggcgc tacgttcgcc gctcaggcag gtgcgacgac caaggagctg
     6241 atggcccgtc tcggtcacac gactcctagg atggcgatga agtaccagat ggcgtctgag
     6301 gcccgcgacg aggctatcgc tgaggcgatg tccaagctgg ccaagacctc ctgaaacgca
     6361 aaaagccccc ctcccaagga cactgagtcc taaagagggg ggtttcttgt cagtacgcga
     6421 agaaccacgc ctggccgcga gcgccagcac cgccgctctg tgcggagacc tgggcaccag
     6481 ccccgccgcc gccaggagca ttgccgttcc cgccagaaat ctagacggtg accacaacgc
     6541 gcccgctttg atcggggacg tcgaaatatt ggatcgtcgg caccgtcacg gccgtgggag
     6601 gcggcacgat ccgcgacgtg atgatcggcc gcatccccac ggtgctgcgc agtgagctct
     6661 acgccatccc ggcgttgatc tgtgcgttcg cacgcacagg cccggtgtga gaagggtctc
     6721 tgacgagcgg gagaactccc tatcag
//
